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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Rh Family C Glycoprotein RHCG Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated for 1 h at room temperature or overnight at 4°C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore, cat ...
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bioRxiv - Pathology 2020Quote: ... IP was performed by incubation of the resulting lysates overnight at 4 °C with mouse monoclonal anti-Flag antibody (Cat.# F3165, Sigma) or with normal mouse IgG (Cat.#A7028 ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 μg of the whole cell extract was rotated overnight at + 4°C with 2.5 μg of the anti-FLAG antibody (Sigma F1804) and the protein-immunocomplexes were separated with the Dynabeads Protein G magnetic beads (ThermoFisher Scientific) ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Primary antibodies were incubated overnight at 4°C and included Col2 (1/100, Thermo) and Sox9 (1/1000, EMD Millipore). Negative controls included a mouse IgG (2µg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated for two nights at 4°C with mouse ACTA2-FITC antibodies (1:200; Sigma, F3777) in blocking buffer containing 1-5% bovine serum albumin in 0.25 – 0.5% Triton-X 100 in 1× PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were subject to 0.1M citrate antigen retrieval at 95°C for 10 mins before the primary antibody for type-II collagen (Millipore, MAB8887), ACAN [30] or lubricin (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... The grids were washed once with 1% BSA in DPBS and incubated with a 1:10 dilution of rabbit anti-c-Myc antibody (Sigma) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... ScAb or mAb samples were incubated with the antigen in doubling dilutions and the binding was detected using anti-Human C Kappa horseradish peroxidase (HRP) conjugated secondary antibody (Sigma) (for scAbs ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 5% nonfat dry milk and incubated overnight at 4°C with primary antibody diluted in PBST–5% nonfat dry milk or 5% bovine serum albumin (BSA, Sigma). Membranes were subsequently washed with PBST and incubated with the appropriate IRDye 680RD and IRDye 800CW secondary antibody (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2019Quote: ... afterwards with secondary antibody mix for 1 day overnight at 4°C and finally with DAPI (1:10000, Sigma-Aldrich) for 8 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-HA (1:2000, Sigma); rabbit polyclonal MBP-probe (1:1000 ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the following primary antibodies for 1 h at room temperature in Abdil: mouse anti-human lamin A/C (1:200; Millipore), rabbit anti-lamin A/C (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in PBS containing 0.3% Triton X-100 and 10% normal donkey serum and incubated with primary antibody overnight at 4 °C overnight (Rockland, rabbit anti RFP, 1:500; Millipore, chicken anti-GFAP ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Biophysics 2020Quote: ... The 3F6H-fused ZTL variants were precipitated (at 4 °C for 10 minutes under dim light) with anti-FLAG antibody (Sigma), which was bound to Protein G-coupled magnetic beads (Dynabeads Protein G ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 1 hour in 3% BSA-TBS and incubated overnight at 4°C with the following primary antibodies: anti-phosphotyrosine 4G10 (Millipore), anti-phosphorylated SYK (pY525/526 ...
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bioRxiv - Genetics 2020Quote: ... All incubations with primary antibodies were performed overnight at 4°C in the presence of protease inhibitors (P2714, Sigma-Aldrich). Secondary antibody incubations were done at room temperature for 1 h protected from light.
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% milk in TBS-T (TBS with 1% Tween-20) and incubated over night at 4°C with the primary anti-FLAG antibody (Sigma F1804-200UG ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in blocking buffer containing 0.2% Tween-20: Puromycin (1:10000, Millipore #MABE343), APOE (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were afterwards incubated with primary antibodies overnight hours at 4 °C at the following dilutions: anti-tubulin β3 (1:1000; Sigma), anti-GFAP (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... To assess the NOTCH3 ECD deposits the tissues were incubated overnight at 4 °C with mouse monoclonal anti-human NOTCH3 ECD primary antibody (1:100, clone 1E4, Millipore) followed by detection with Alexa 594 conjugated anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
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bioRxiv - Physiology 2022Quote: ... or overnight incubation at 4°C with a primary antibody for laminin (Millipore-Sigma; Cat. No. L9393, 1:100 dilution) following with Alexa-Fluor secondary antibody incubation (ThermoFisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... or overnight incubation at 4°C with a primary antibody for laminin (Millipore-Sigma; Cat. No. L9393, 1:100 dilution) following with Alexa-Fluor secondary antibody incubation (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% triton-X 100 in PBS at room temperature for one hour and incubated overnight in 5% FBS and 0.1% triton-X 100 in PBS at 4°C with the following antibodies: anti-LIMCH1 (1:200, catalog number HPA063840, Sigma), anti-mLIMCH1 (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 20μL antibody (anti-FLAG; Sigma F1804-1MG). 30×10^6 of ESCs crosslinked with formaldehyde as described above were resuspended in 500μL RIPA Buffer (50mM Tris-HCl ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Physiology 2023Quote: ... sections were exposed overnight at 4°C to antibodies detecting CARNS1 (rabbit polyclonal, 1:100 in PBS with 1% BSA, HPA038569, Sigma). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for 1h at room temperature in blocking solution (5% non-fat dried milk and 0.1% Tween 20 in PBS) and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated overnight at 4°C with 10μL antibody (anti-SAFB; Bethyl 812-300A; FLAG, Sigma F1804; V5, Sigma V8012) or 10μg of mouse IgG (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 300 g for 10 min and incubated for 25 min at 4°C with the primary antibody from A2B5 (Millipore) and/or O4 hybridoma cells (DSHB ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated 36 hours at 4°C with the primary antibody – anti-γH2AX (Ser139) clone JBW301 (1:500, 05-636-I, Millipore) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated overnight at 4 °C with a rat monoclonal anti-poly(GR) antibody (clone 5A2, MABN778, Millipore), a mouse monoclonal anti-poly(GA ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween-20 and 5% non-fat milk for 1 hour at room temperature and probed for 18 hours at 4 °C with antibodies specific for Flag tag (M2, SIGMA), Myc tag (9E10 ...