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Citations for Millipore Sigma :
51 - 100 of 7421 citations for Recombinant Zebrafish tnfa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 1000 units/ml ESGRO Recombinant Mouse LIF protein (Millipore #ESG1107), 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... then tested against recombinant proteins coated in Dulbecco’s PBS (DPBS, Sigma-Aldrich) overnight onto 96-well Immunlon-2 plates (VWR Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Biochemistry 2023Quote: ... They were incubated with recombinant ULK1 proteins (Sigma, #SRP5096-10UG, Darmstadt, Germany) in the kinase buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... All zebrafish experiments were stopped at day 9 post-infection and zebrafish were euthanized with tricaine (MS-222) (Sigma-Aldrich #E10521). Each experiment was repeated at least 3 times and between 10 and 15 larvae were used per condition and per experiment.
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bioRxiv - Developmental Biology 2021Quote: ... zebrafish and the δ-cell ablation model Tg(sst2:NTR) zebrafish in E3 medium supplemented with 10 mM metronidazole (MTZ, Sigma-Aldrich), 1% DMSO (VWR ...
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bioRxiv - Developmental Biology 2021Quote: Zebrafish were anaesthetised using Tricaine (MS-222, Sigma-Aldrich) and embedded in 1% low-melting point agarose which was held in place in a glass capillary and imaged using a light sheet Z.1 microscope (Zeiss) ...
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bioRxiv - Cell Biology 2021Quote: ... we treated zebrafish embryos with 60 μM norepinephrine (Sigma) in E3 fish water containing 0.003% PTU ...
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bioRxiv - Cell Biology 2021Quote: ... we treated zebrafish embryos with 4x tricaine (0.32%, Sigma) in E3 embryo medium with 0.003% PTU (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: Zebrafish embryos were anesthetized with tricaine (Sigma-Aldrich, # A5040) at a concentration of 0.164 mg/ml and mounted in 1.5% low gelling temperature agarose (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: Zebrafish embryos were anesthetized using MS-222 (Sigma-Aldrich) and imaged on an M165FC fluorescent stereomicroscope (Leica ...
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bioRxiv - Immunology 2020Quote: ... zebrafish larvae were exposed to 10mM BrdU (Sigma-Aldrich) in system water for 24 hours prior to fixation at 9dpf/4dpi ...
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bioRxiv - Cell Biology 2020Quote: ... Adult zebrafish were anaesthetized in 0.02% MS-222 (Sigma) and injected intra-peritoneally with 2×104 M ...
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bioRxiv - Developmental Biology 2024Quote: ... Zebrafish brain samples were lysed in RIPA buffer (Sigma) with 1/100 antiprotease and sonicated for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Zebrafish embryos were anesthetized in 0.02% tricaine (Sigma-Aldrich) at 2-day post fertilization (dpf ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant proteins were produced and purified on glutathione-agarose column (G4510, Sigma) for GST-tagged protein or on TALON-Cobalt beads column (635507 ...
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bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Genetics 2020Quote: Adult zebrafish were euthanized using tricaine (MS-222, Sigma-Aldrich). Tissue was fixed in 4% PFA prior to microCT analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... zebrafish were euthanized in 0.2% Tricaine (MS-222, Sigma E10521) solution ...
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bioRxiv - Developmental Biology 2022Quote: Zebrafish embryos and larvae were treated with phenylthiourea (PTU; Sigma) from 24 hpf onwards in order to induce hypothyroid conditions (Elsalini and Rohr ...
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bioRxiv - Developmental Biology 2022Quote: ... Zebrafish were sacrificed by immersing in 0.02% MS222 (Sigma, E10505) until no opercular movement ...
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bioRxiv - Neuroscience 2020Quote: ... zebrafish embryos were terminally anaesthetised in MS222 tricaine (Sigma-Aldrich) and incubated ...
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bioRxiv - Immunology 2022Quote: ... Frozen zebrafish adult brains were lysed in RIPA buffer (Sigma), 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Developmental Biology 2021Quote: ... Zebrafish were anesthetized using 0.0168% buffered tricaine (MS-222, Sigma). AB strains of zebrafish were used as wild-type for this study ...
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bioRxiv - Genetics 2020Quote: The zebrafish were euthanized in tricaine (MS-222, Sigma-Aldrich) and fixed for 3 days in 4% paraformaldehyde at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... zebrafish larvae were anaesthetised with 0.017% MS-222 (Sigma-Aldrich). For total fluorescence acquisition ...
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bioRxiv - Microbiology 2020Quote: Zebrafish were euthanized with tricaine (MS-222) (Sigma-Aldrich #E10521) at 0.3 mg/mL for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Zebrafish embryos were homogenised by addition of Trizol (Sigma, USA) and passed through a QIAshredder column (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... fixed zebrafish larvae firstly treated with acetone (650501, Sigma-Aldrich) at −20 °C for 7 min ...
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bioRxiv - Genetics 2022Quote: ... stained zebrafish larvae were anesthetized in 0.01% MS-222 (Sigma) and mounted into borosilicate glass capillaries using 0.75% low melt-agarose (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... zebrafish were euthanized with tricaine (MS-222, Sigma-Aldrich E10521) at 0.3 mg/mL ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish embryos were treated with 10 μM DMH1 (Sigma Aldrich) or 100 μM cyclopamine (Cayman Chemical ...
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bioRxiv - Microbiology 2023Quote: Zebrafish embryos were anesthetized using 0.04% Tricaine (Sigma-Aldrich, E10521) for experimental procedures ...
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bioRxiv - Developmental Biology 2024Quote: ... zebrafish were bathed in 5 µM tamoxifen (Sigma-Aldrich, T5648) for 24 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... Zebrafish embryos were fixed overnight in 4% PFA (47608, Sigma) in PBS (EC-A500 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant protein was then concentrated using centrifugal filter units (Amicon Ultra-4, Millipore) and dialyzed using Slide-A-Lyzer dialysis cassette into storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Purified recombinant S proteins were concentrated using Amicon filtration devices (EMD Millipore, Billerica, MA), buffer-exchanged into PBS and analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was purified by affinity chromatography using anti-FLAG affinity resin (Sigma). The purified protein was precipitated by 20% trichloroacetic acid ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: We concentrated recombinant EGFP fusion proteins using Amicon Ultra centrifugal filters (10K MWCO, Millipore) to an appropriate protein concentration in 250 mM NaCl salt buffer ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).