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Citations for Millipore Sigma :
301 - 350 of 7421 citations for Recombinant Zebrafish tnfa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Cancer Biology 2022Quote: ... 7μL of each dilution was then used in SDS-PAGE alongside a dilution series of recombinant GST protein (Sigma-Aldrich #SRP5348) from 5ng-200ng ...
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bioRxiv - Microbiology 2023Quote: ... mixture of BEI-EMCV strain SING-M105 or recombinant VP1 protein prepared according or BEI-EMCV with Complete Freund’s adjuvant (Sigma-Aldrich, USA) was injected intramuscularly into five BALB/c mice at the concentration of 50 μg of antigen per mouse ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Bioengineering 2023Quote: ... The hybridized RNAs were then mixed with 25 µL of 30 µM (5 mg/mL) recombinant SpCas9 protein (Sigma-Aldrich #Cas9PROT) for 30 minutes at 4 C ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Neuroscience 2022Quote: ... and 1μM recombinant human insulin (Sigma). Organoids were collected from the wells on day 14 and transferred to 10cm dishes at roughly 20 organoids per dish ...
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bioRxiv - Physiology 2022Quote: ... 500μg/ml human recombinant albumin (Sigma), 213μg/ml L-ascorbic acid (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... and recombinant chondriotinase ABC (Sigma, C3667) was treated at 25 mU/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant active AMPK (Millipore, 14-840) was added and incubated for 30 min at 30°C ...
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bioRxiv - Immunology 2020Quote: ... human recombinant TNF (H8916, Sigma-Aldrich) or chemical inhibitors (SB203580 from Selleck ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant IFNα was purchased from Sigma (Interferon-αA/D human Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... and Recombinant mouse LIF (Sigma, #ESG1107). Additionally ...
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bioRxiv - Immunology 2021Quote: ... Recombinant purified Angiotensin II (Sigma – A9525) at 1 μM with 2-fold serial dilutions ...
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bioRxiv - Immunology 2022Quote: ... recombinant complement component C3 (Millipore Sigma), recombinant CD4 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant leukemia inhibitory factor (SRP3316; Sigma), growth hormone (869008 ...
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bioRxiv - Neuroscience 2023Quote: ... and/or recombinant Fibronectin (Sigma-Aldrich) were applied as described using a range of concentrations (μg/ml).
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bioRxiv - Biophysics 2023Quote: ... superoxide dismutase (bovine SOD, recombinant, Sigma) was added from a ≥50,000 U/mL stock prepared in assay buffer ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant human TNFα (Sigma Aldrich, #H8916), PGE2 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: BG4 recombinant antibody (Sigma Aldrich MABE917)- ChIP
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bioRxiv - Developmental Biology 2024Quote: ... 10nM recombinant human gastrin (Sigma; G9145), 5ng/mL recombinant human HGF (PeproTech ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant hCG (Sigma), and 10 mg/ml of human serum albumin (Sage) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000U/ml ESGro recombinant LIF (Millipore), 1uM PD0325901 (R&D Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... UAS:GCaMP7a expression was used to activate TRPV1 channels in zebrafish by incubation in 5μM capsaicin (Sigma, M2028) and subsequent live imaging for 1hour of the mounted and anesthetized embryo ...
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bioRxiv - Developmental Biology 2021Quote: ... genomic DNA was isolated from 5 individual 72hpf zebrafish embryos using Chelex 100 sodium form (Sigma Aldrich). Briefly ...
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bioRxiv - Immunology 2022Quote: ... 2- or 3-days post fertilisation (dpf) zebrafish were anaesthetised in 0.168 mg/ml Tricaine (Sigma-Aldrich) in E3 media and visualised under a dissecting microscope ...
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bioRxiv - Immunology 2022Quote: ... Zebrafish were anaesthetised in 0.168 mg/ml Tricaine and mounted vertically in 1% low melting agarose (Sigma) in a glass capillary ...
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bioRxiv - Cell Biology 2022Quote: Larval zebrafish were maintained at 28.5°C in E3 medium with 0.003% N-Phenylthiourea (Sigma Aldrich, P9629) to prevent pigmentation ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged zebrafish Grk1a or Grk1b was isolated using anti-FLAG M2 Affinity Gel (#A2220) from Millipore-Sigma as described previously (15 ...
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bioRxiv - Genetics 2019Quote: Zebrafish embryos were anesthetized with E3/0.16 mg/mL tricaine/1% 1-phenyl-2-thiourea (Sigma, USA) and embedded in 0.8% low melt agarose ...
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bioRxiv - Cell Biology 2020Quote: Zebrafish embryos were treated with the dynein inhibitor ciliobrevin D (CBD)(Calbiochem via Sigma-Aldrich, 250401-10MG)(2.5 μM in 0.1% DMSO ...
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bioRxiv - Bioengineering 2020Quote: ... Zebrafish were sacrificed with a lethal dose of MS-222 (Ethyl 3-aminobenzoate methanesulfonate) (Sigma-Aldrich, UK), followed by washing with 0.5% sodium hypo chloride or bleach (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: Adult zebrafish were euthanized with 200 – 300 mg/L of ethyl 3-aminobenzoate methanesulfonate (tricaine) (Sigma, E10521) prior to dissection ...
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bioRxiv - Immunology 2020Quote: WT and gpr35buu1892 zebrafish larvae were either left unstimulated or stimulated with 10 μM LPA (Sigma L7260) in water from 96 hpf until 120 hpf ...
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bioRxiv - Immunology 2020Quote: ... rSpike was injected into two immunization sections in 20 zebrafish females (previously anesthetized with tricaine methanesulfonate (Sigma) - at a dose of 150 mg/L ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish larvae were exposed to a solution of 10 mM metronidazole (Sigma-Aldrich, cat. number M3761-100G) in standard fish water ...
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bioRxiv - Neuroscience 2023Quote: ... zebrafish larvae were placed into individual wells of a 96- square well plate (WHA7701-1651 Sigma-Aldrich) filled with 650 μl of embryo water per well and tracked for 3 days under a 14:10 light:dark schedule (lights on-09:00 ...
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bioRxiv - Neuroscience 2023Quote: Zebrafish embryos were paralyzed in 0.02% tricaine and mounted on 2% low melting-point agarose (Sigma-Aldrich). Optical sectioning was performed using an Olympus FV1000 or a Nikon A1R (detector ...
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bioRxiv - Developmental Biology 2023Quote: ... Four 4dpf zebrafish embryos were placed in 96-well square bottom Whatman Uniplate multiwell plates (Sigma-Aldrich) in 300µl of E3 medium ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish embryos were raised in 0.003% 1-phenyl-2-thiourea (PTU, phenylthiocarbamide, or n-phenylthiourea, Sigma P7629) from gastrulation stage to prevent pigmentation ...
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bioRxiv - Neuroscience 2024Quote: ... A single zebrafish larva was transferred to bath solution (see below) containing tricaine (0.02%, MS222, Sigma Aldrich) for 1 minute to anesthetize it for dissection ...
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bioRxiv - Cancer Biology 2024Quote: ... Zebrafish embryos at 48 hpf were then dechorionated when required and anesthetised with tricaine (Sigma-Aldrich, USA). Using Nanoject II (Drummond Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... P3 2M (AMS biotechnology #GSC-6201G 2M or #GSC-6101G 7M) and LIF ESGRO® Recombinant Mouse LIF Protein (1000 units/mL) (Millipore # ESG1107). For induction of the transgene Stemolecule Doxycycline hyclate 10 mg (Stemgent#04-0016 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Genetics 2021Quote: 48 hours post-fertilization (hpf) zebrafish larvae obtained from tert +/- breeding crosses were anesthetized in 0.008% tricaine (Sigma) and placed on an agar plate to amputate the caudal fin primordia with a surgical razor blade just posterior to the notochord ...
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bioRxiv - Developmental Biology 2021Quote: ... we treated Tg(ins:Flag-NTR);Tg(ubi:Switch);Tg(drl:CreERT2) zebrafish embryos with 10 μM 4-OHT (Sigma-Aldrich) in E3 medium in 90-mm Petri dishes ...