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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Recombinant Mouse PVR Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: Localization of tagged proteoforms in embryos was visualized by immunohistochemistry using Mouse Anti-FLAG M2 (F1804 Sigma), followed by biotinylated goat anti-Mouse IgG (BA-9200 ...
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bioRxiv - Genetics 2024Quote: ... APOBEC3C tagged with the superFLAG epitope was detected by immunoblotting with an anti-FLAG mouse antibody (Sigma anti-FLAG M2 antibody F3165 ...
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bioRxiv - Developmental Biology 2023Quote: ... This basal medium was completed with 5ng/ml recombinant mouse LIF (Sigma ref LIF2050), 3µM CHIR99021 (Sigma ref SML1046-5MG ...
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Physiology 2019Quote: ... GST-free eluates of protein were prepared by using 1 unit of biotinylated thrombin (Millipore 69672-3) per 100 μl of beads and letting samples incubate on a rotator at 4° C overnight ...
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bioRxiv - Biophysics 2020Quote: The RBD and spike proteins were chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). For the RBD ...
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bioRxiv - Biochemistry 2022Quote: Biotinylated proteins were digested with 250 ng of trypsin mass spectrometry grade (Sigma-Aldrich, St. Louis, MO) in 50 mM of ammonium bicarbonate (ABC) ...
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bioRxiv - Plant Biology 2023Quote: ... Labelled proteins were biotinylated with 20 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described in “Labeling and click chemistry” ...
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bioRxiv - Plant Biology 2024Quote: ... Labelled proteins were biotinylated with 30 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich Inc.) were used ...
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bioRxiv - Cell Biology 2019Quote: ... The biotinylated probe used was generated by PCR using biotinylated-16-UTP (Sigma) and primers P3 and P4 ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Microbiology 2021Quote: ... The expression of S-tagged NrdA was confirmed by purification using S-protein agarose (Merck Millipore, Darmstadt, Germany) as previously described (11 ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... The GST-tagged proteins were eluted by 10mM reduced glutathione (Sigma, 100mM stock made in 50mM Tris, pH7.6) in PBS (containing 1mM PMSF and 0.02% (∼3mM ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged protein was eluted at room temperature with 25 μl myc-peptide (0.5mg/mL in PBS, Sigma) for 15 minutes at 700 rpm mixing ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins was performed with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, cat.no. A2220-1ML) at 4 °C with overhead rotation for 6 h ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged protein was eluted off the beads by using 20 mM glutathione solution (Sigma-Aldrich, #G4251-5G). Fractions were analyzed by resolving on 4-20 % gradient SDS-PAGE (BioRAD #4561094 or #4651093) ...
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Bioengineering 2022Quote: ... pSANG10-Avi clones were expressed in the same way in the presence of 50μM free D-biotin (Sigma).
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bioRxiv - Plant Biology 2019Quote: ... The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore). Protein yield was estimated by measuring absorbance at 595 nm using a spectrophotometer (UV-2600 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... subcutaneously with 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) followed by a boost after 4 weeks ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of recombinant protein was induced by 0.2% (w/v) L-Arabinose (Sigma Aldrich). The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.
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bioRxiv - Neuroscience 2021Quote: ... The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220; Sigma-Aldrich Japan), eluted with FLAG peptide (A3290 ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was briefly vortexed in the presence of 1% Triton X-114 (Sigma), incubated on ice for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Flag-hSpt16 proteins were incubated with 12.5 U of recombinant ULP-1 SUMO Protease (Sigma) for 16 h at 4°C to remove SUMO moieties.
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified recombinant proteins were analyzed by SDS-PAGE and concentrated using stirred ultrafiltration cells (Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant proteins (PRMT5:MEP50 complex (SRP0145-25470) and EZH2 (SRP0379) were purchased from Sigma-Aldrich USA ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
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bioRxiv - Bioengineering 2021Quote: ... Four different target Fc-proteins were used in this study: hIgG (Sigma-Aldrich, St. Louis, MO), plant-expressed rCMG2-Fc ...
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bioRxiv - Cell Biology 2023Quote: ... Secreted JAGGED1-Fc was recovered from the culture media on Protein A agarose (Millipore, 16-125) and eluted with 100 mM glycine ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Cell Biology 2023Quote: ... biotinylated proteins were selectively eluted by incubating the beads in 100 µL of 5 mM dithiothreitol (DTT, Sigma), 5% SDS ...
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bioRxiv - Genetics 2019Quote: ... 10% FCS (Sigma) supplemented with FLT3L (50 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 % FCS (Sigma) and P/S (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... + 10% FCS (Sigma,) + 1 x (final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... 10% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... 5 % FCS (Sigma) in DPBS for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FCS (Sigma), NEAA ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant MEK and recombinant ERK were purchased from Sigma. Indicated amounts of recombinant GST-HSF-1 (0.1–0.4 mg ...
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bioRxiv - Genomics 2020Quote: Biotinylated ConA (C2272, Sigma-Aldrich) and streptavidin (CS10471 ...
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bioRxiv - Neuroscience 2020Quote: ... and Biotinylated HABP (Millipore, 385911) was performed in PBS + 1% BSA with no detergent ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated hTf (bio-hTf; Sigma) at concentrations ranging from pM to µM in 0.1% BSA in PBST was added to the wells ...
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bioRxiv - Biophysics 2023Quote: ... or biotinylated transferrin (Sigma-Aldrich) at 37°C with a molar ratio of 1:3 for 1h ...