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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Recombinant Mouse PVR Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged proteins were probed with immunoblotting using monoclonal anti-Flag M2-Peroxidase (HRP) antibody (Sigma-Aldrich, Saint Louis, MO, USA) (1/50,000X dilution ...
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bioRxiv - Genetics 2019Quote: FLAG-tagged proteins were immunoprecipitated from 2 mg of HEK293T or M17 cells using anti-FLAG M2 affinity gel (Sigma A2220). Lysates were prepared in NP40 buffer (see Western blotting) ...
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bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... The EGFP-FLAG-tagged proteins were eluted from the beads with 0.1 mg/mL 3x FLAG peptide (Sigma-Aldrich, Cat. #F4799) in TBS at 4°C for more than 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... The FLAG-tagged protein-DNA complex was purified via affinity chromatography using anti-FLAG M2 affinity gel (Sigma-Aldrich, Taufkirchen, Germany). The purified protein-DNA complexes and the control were incubated at 95 °C for 40 min for reversal of the crosslink ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged protein was precipitated with 20 μl anti-V5 beads (Anti-V5 Agarose Affinity Gel, Cat.- No. A7345, Sigma-Aldrich) rotating on a wheel for 2 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... MBP- and GST-tagged proteins were purified by gel filtration on a Superdex 200 column (Sigma-Aldrich Cat# GE17-5175-01) and concentrated using a 30K Amicon filter.
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Microbiology 2020Quote: ... 100 μg/ml of biotinylated HA (Sigma) was added to each well and incubated for 1 h at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... stored and biotinylated using biotin-NHS (Sigma) as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... or 1:1500 biotinylated IB4 (Sigma, L2140) while shaking at 4 °C overnight in solution with 0.2% Triton X-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... Biotinylated porcine intestinal mucosa heparin (Merck Millipore) was immobilized on streptavidin biosensors at 10 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... biotinylated WFA (1:1000, Sigma, cat# L1516), rabbit anti-BCAN (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... IB4 biotinylated (Sigma Aldrich, L2140, 1:200). Sections were washed in PBS for 5 minutes three times ...
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bioRxiv - Neuroscience 2023Quote: ... and biotinylated WFA (1/100, Sigma L1516). Secondary antibodies were Alexa Fluor-conjugated (Molecular Probes) ...
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bioRxiv - Neuroscience 2020Quote: ... and manual application of 100μl of directly biotinylated mouse monoclonal IgG1 antibodies against Aβ (82E1, IBL, 0.2 μg/ml or 4G8, Millipore, 2 μg/ml) for 8 hours ...
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bioRxiv - Neuroscience 2019Quote: ... tissue was incubated in secondary antibody (Biotinylated horse anti-mouse IgG 1:500, BA-2001, Vector Laboratories, Burlingame, CA; biotinylated donkey anti-goat IgG 1:500, AP180B, Millipore-Sigma ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... resuspended in RPMI-10% FBS containing 40ng/mL CTLA4-Ig (CD152/Fc chimera, non-cytolytic, from mouse, Sigma C4358), plated in 24-well culture plates at a density of 2 x 106 cells/well and cultured for an additional 24 hours (BMDCCTLA4-Ig) ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Immunology 2021Quote: ... with 10% FCS (Sigma-Aldrich) and 100 U/ml penicillin plus 100 mg/ml streptomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Fc IgG (AG714, Merck Millipore) at 2 μg/ml in 150 μl of PBS for one hour ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FCS (Sigma), 2mM L-glutamine (Thermofisher) ...