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Citations for Millipore Sigma :
401 - 450 of 8779 citations for Recombinant Influenza A virus HA protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... The presence of immunocomplexes was assessed by probing protein gel blots with either anti-HA (3F10, Sigma/Roche; http://www.sigmaaldrich.com/) or anti-Myc antibody (9E10 ...
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bioRxiv - Microbiology 2020Quote: ... The analyzed proteins contained an SHH-tag (Sarkari et al., 2014) and were detected using anti-HA (Sigma-Aldrich, USA) antibodies and anti-mouse IgG-HRP (Promega ...
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bioRxiv - Microbiology 2020Quote: ... the bound proteins were eluted with 400 μL of wash buffer supplemented with 250 μg/mL HA peptide (Sigma-Aldrich) by incubation at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads-antibody-protein mix was incubated overnight at 4°C and bounds protein were eluted with 35 μg HA peptides diluted in PBS (Sigma); then Laemmli buffer was added and the eluate heated at 95°C 2 min.
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bioRxiv - Immunology 2023Quote: ... The protein was then eluted in 15 μL of peptide elution buffer three times using Flag peptide or HA peptide (Sigma) 300µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation was achieved by incubating cell lysates containing 1 mg of protein overnight with anti-HA agarose beads (Sigma-Aldrich), Myc-agarose beads (Santa cruz biotechnology ...
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bioRxiv - Immunology 2023Quote: ... for 2h at 4°C and washed four times and precipitated protein were competitively eluted with HA-peptide (100ng/ml; I2149, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... 1:1000 anti-HA Mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma Aldrich) or 1:1000 anti-ACP antibodies (a kind gift from Prof ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal anti-HA antibody-conjugated agarose beads (mouse monoclonal anti-HA-agarose antibody) (Sigma #A2095) were added to the cleared extracts and incubated at 4°C for 4 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Biochemistry 2022Quote: ... pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich, catalog no. H6908) (3.5 μg antibody per reaction) ...
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bioRxiv - Cell Biology 2019Quote: ... 1:1000 anti-HA (monoclonal, Clone HA-7, mouse IgG1 Isotype, from Sigma Aldrich®) or 1:1000 anti-ACP antibodies (obtained as a kind gift from Prof ...
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bioRxiv - Systems Biology 2020Quote: ... Anti-HA (clone HA-7) and anti-flag M2 (clone M2) antibodies were from Sigma. m6A RNA methylation quantification kit was from Abcam(ab185912).
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bioRxiv - Immunology 2020Quote: ... RH or RHtgd057-HA were stained with rat anti-HA primary antibody (Sigma, clone 3F10) at 1:500 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-HA (Monoclonal Anti-HA antibody produced in mouse, H9658 Sigma-Aldrich, 1:1000) and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:1000 anti-HA mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma-Aldrich), or 1:1000 anti-ACP antibodies (a kind gift from Prof ...
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bioRxiv - Plant Biology 2024Quote: ... and the immuno-complexes was then analysed on a 10% SDS-PAGE gel using immunoblotting methods with Abcam (Cambridge, UK) anti-GFP and anti-HA antibodies (Anti-HA High Affinity Roche 3F/10, Sigma-Aldrich, and anti-HA Abcam). At least three independent biological replicates were performed per experiment.
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bioRxiv - Plant Biology 2024Quote: ... The immuno-complexes was then analysed on a 10% SDS-PAGE gel using immunoblotting methods with anti-HA antibodies (Anti-HA High Affinity Roche 3F/10, Sigma-Aldrich, and anti-HA Abcam). At least three independent biological replicates were performed per experiment ...
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bioRxiv - Microbiology 2023Quote: ... and influenza virions were concentrated and purified by a two-step ultracentrifugation through a cushion and a gradient of OptiPrep (Sigma) in NTC buffer (100 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: Recombinant Fetuin-A (Sigma; SRP6217) was diluted in 0.9% saline vehicle solution ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant PAK1 (Millipore, 14-927) and agitated at 30°C for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant bacterial sphingomyelinase (S7651, Sigma); 2-bromopalmitate (320-76562 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse recombinant IFN-β (Millipore) diluted in DPBS with 0.1% Bovine serum albumin BSA (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Genetics 2019Quote: ... 15 U recombinant DNase1 (Sigma) and 10 U DNA polymerase I (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant DNAse I (Sigma) in MACS buffer (PBS/2mM EDTA/ 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
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bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
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bioRxiv - Microbiology 2021Quote: Recombinant mouse adiponectin (Sigma, #SRP3297) and GFP protein (Sino Biological ...
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Physiology 2022Quote: Lyophilised recombinant Aβ42 (#AG912, Millipore) and scrambled control peptide (ScrAβ42 ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: Recombinant Mpro (Sigma Aldrich, SAE0172) was resuspended in 10% aqueous glycerol to a concentration of 1 mg/mL ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Epidemiology 2019Quote: ... and recombinant heat labile toxin β subunit protein [15,52,53] from enterotoxigenic Escherichia coli (ETEC LT β subunit) were purchased from Sigma Chemical (St ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Biochemistry 2022Quote: ... and fractions judged by SDS-PAGE to contain the recombinant protein were pooled and desalted by size exclusion chromatography using 30K centrifugal spin column (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Microbiology 2020Quote: Female BALB/c mice have been injected intraperitoneally with 700 μg recombinant His-tagged TGT protein that was emulsified in complete Freund’s adjuvant (Sigma-Aldrich). Two weeks later ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...
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bioRxiv - Plant Biology 2019Quote: ... using a VP-ITC microcalorimeter (MicroCal) as described [34] after extensive dialysis of the purified recombinant protein in Chelex 100 (Sigma) to remove contaminating divalent cations ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cell Biology 2019Quote: ... Immune sera were generated by immunization of 6 weeks-old female NMRI mice (Charles Liver Laboratories) via subcutaneous injection of 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks with 50 µg of recombinant protein ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biophysics 2021Quote: Catalytic activity was evaluated in vitro using 20 pmol of purified recombinant proteins in a 200 µl reaction buffer supplemented with 20 mM p-nitrophenyl phosphate (Sigma) as substrate ...