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Citations for Millipore Sigma :
451 - 500 of 8779 citations for Recombinant Influenza A virus HA protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were transfected with HA-PFKP which was immunoprecipitated with anti-HA agarose (Sigma-Aldrich) 48 hours after transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by immunoprecipitation with Anti-HA Agarose beads (Sigma-Aldrich EZview Red Anti-HA Affinity Gel). The precipitated protein complex was detected for interaction partners by mass spectrometry and the results obtained were analyzed by MaxQuant computational platform.
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bioRxiv - Cell Biology 2021Quote: ... fixed and processed by immunofluorescence with an anti-HA monoclonal antibody (clone HA-7, Sigma Aldrich) and a secondary anti-mouse AlexaFluor 555 (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections were immunostained with anti-HA (1:100; Sigma-Aldrich anti-HA high affinity-clone 3F10) and anti-PYY (1:400 Abcam-Ab1 ab22663 ...
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bioRxiv - Biophysics 2023Quote: ... The migration of the synthesized HA species was compared to HA standards from Streptococcus equi (Sigma), as well as enzymatically generated ladders ...
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bioRxiv - Microbiology 2023Quote: ... ORF3c-HA was detected using a primary antibody against the HA-tag (Sigma-Aldrich, Cat# H3663) followed by staining with a secondary AF555 antibody (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... electrophoretically transferred onto PVDF transfer membranes (ThermoFischer Scientific) and stained for HA (Sigma-Aldrich, HA-7), V5 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Genetics 2019Quote: Cell extracts were prepared by TCA precipitation and proteins were separated by 10% SDS-PAGE followed by immunoblotting with anti-HA antibody (Sigma-Aldrich, ref ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were adjusted to 1-2 mg total protein in 1 mL and incubated overnight at 4°C with 25 µL of packed Monoclonal Anti-HA Agarose (Sigma-Aldrich). Following incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then added to the Protein G sepharose beads coupled to either HA 12CA5 mouse monoclonal IgG (Sigma Aldrich) or anti-Ty1 (BB2 ...
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bioRxiv - Plant Biology 2020Quote: ... 30 confirmed transgenic plants were screened for dPPRrbcL expression by immunoblot on leaf total protein extracts using HA antibodies (H9658 clone, Sigma-Aldrich) and three lines expressing gradual levels of dPPRrbcL protein were chosen for phenotypic analyses.
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bioRxiv - Cell Biology 2024Quote: ... Equal concentration of total protein extracts were incubated with Myc (#1-9E10.2 hybridoma supernatant, ATCC, USA) or HA (#11583816001, 12CA5, Sigma-Aldrich, USA) antibodies for 2h at 4°C on a rotator ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... or HA affinity agarose (A2095, Millipore) overnight at 4° C ...
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bioRxiv - Cell Biology 2020Quote: ... anti-HA antibody (12CA5; Roche/Sigma) was used to detect Mps2-3HA ...
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bioRxiv - Cell Biology 2019Quote: ... or anti-HA beads (Sigma, E6779) for 3 hrs at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... HA-tag (Sigma-Aldrich# H-9658), FLAG (Sigma-Aldrich#) ...
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bioRxiv - Microbiology 2019Quote: ... rabbit anti-HA (Sigma, Cat. # H6908). Primary antibodies were labeled with fluorophore-conjugated donkey anti-mouse or anti-rabbit antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... An α-HA antibody 3F10 (Sigma) was added for another 4 hours (1:1,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5μg of anti-HA (Sigma H6908) or anti-IgG (Fisher 02-6102 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse α-HA (Sigma, 1:400), mouse α-Bruchpilot (Developmental Studies Hybridoma Bank nc82 ...
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bioRxiv - Cell Biology 2019Quote: ... or anti-HA agarose (Sigma A2095) washed 3 times in lysis buffer and subjected to SDS-PAGE and western blot ...
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bioRxiv - Cell Biology 2019Quote: ... anti-HA (cat#: 11867423001, Sigma-Aldrich), anti-GFP (cat:# ab13970 ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-HA (1:1,000; Sigma), rabbit anti-TH (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-HA (1:1000, Sigma), rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... rabbit anti-HA (1:100, Sigma), and rabbit anti-Sendai virus (SV ...
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bioRxiv - Neuroscience 2019Quote: ... rat anti HA (1:500, Sigma); mouse anti TH (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-HA agarose conjugate (Sigma Aldrich) was used.
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bioRxiv - Plant Biology 2020Quote: ... or α-HA (H9658; Sigma-Aldrich) antibody.
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bioRxiv - Microbiology 2021Quote: ... or Anti-HA Agarose (Sigma Aldrich) for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... HA (Cat. #05-904-Merck Millipore); cleaved-caspase-3 (9H19L2-Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-HA (1:2000, 3F10, Sigma), and anti-FLAG (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... mAb-HA (Clone 7, H9658, Sigma), pAb-HA (Signalway Eurogentec) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-HA (Sigma, 1:1000), rabbit anti-HA (CST ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tag (HA.C5, Millipore 05-904) or FLAG-tag (M2 ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse anti-HA (SIGMA, H3663). Dilutions are detailed in the table below.
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bioRxiv - Plant Biology 2020Quote: ... HA (Sigma, St. Louis, Missouri, US) or Myc (Sigma) ...