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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... were obtained using terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay (82) (based on in Situ Cell Death Detection Kit, 11684795910, red fluorescence; Sigma-Aldrich) on sections derived from rd1 and C3H retinal explant cultures ...
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Developmental Biology 2023Quote: TUNEL assays were performed after secondary antibody staining by using the In Situ Cell Death Detection Kit TMR red (Millipore #12156792910). Embryos were incubated for 4 hours at room temperature (RT ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was assayed between 20-24 hours post-treatment for lactate dehydrogenase (LDH) activity as a marker of cell death using a commercially available kit (Tox7, Sigma-Aldrich) as previously described (Aras et al. ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Biochemistry 2019Quote: ... Following 12 h recombinant expression in Rosetta Competent Cells (Millipore, #70954) at 12° C with ampicillin and chloramphenicol selection ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Immunology 2022Quote: ... cells were then stimulated with 100U/ml recombinant IFNα2 (Sigma-Aldrich) to induce transcription from the IFIT1 loci ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant E1E2 was extracted from cell membranes using 1% NP-9 and purified via sequential Fractogel EMD TMAE (Millipore), Fractogel EMD SO3− (Millipore) ...
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bioRxiv - Genomics 2019Quote: The human K562 cells(Sigma-Aldrich) were grown at 37 °C in RPMI 1640 medium (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... human cardiomyocyte AC16 cells (Millipore; SCC109) in DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Human bronchial epithelial cells (16HBE14o-, Sigma) were maintained in medium consisting of α-MEM (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Genomics 2022Quote: ... FITC-tagged NeuN antibody (Sigma, MAB377) was prepared following manufacturer’s instructions (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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bioRxiv - Developmental Biology 2019Quote: ... Human cells were detected using directly Cy3-conjugated anti-HNA (Millipore, MAB1281C3, 1:100). After washing in TBS ...
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bioRxiv - Cell Biology 2020Quote: ... Immunofluorescence in human cells was conducted using antibodies against mouse □-tubulin (Sigma, 1:1000), mouse CENP-E (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Human microvascular endothelial cells (HMEC-1) were cultured in MCDB-131 medium (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then treated with ligands (10 nM DAMGO, 30 nM morphine) and 2.5 μM Forskolin (FSK, Sigma-Aldrich F6886), followed by continuous luminescence imaging for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: Mouse (5 x 105 cells) and human (2 x 105 cells) keratinocytes were seeded onto 12 mm diameter inserts (Millipore, Catalog No. PIHP01250) and cultured in CnT-Prime medium ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... an additional blocking step was performed by incubating cells with 2 mg/ml γ-globulins from human blood (Sigma) for 30 min at 37°C ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2021Quote: ... HMEC-1 cells (human microvascular endothelial cells RRID:CVCL_0307) were grown in HMEC media (MCDB 131 media (Sigma, M8537) supplemented with 10% FBS (HyClone ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Microbiology 2023Quote: ... The cells were overlaid with 1:1 volume of 2 % methyl cellulose (Sigma, USA) in 2X MEM supplemented with 5 % FBS and plate was incubated for 48 h ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Microbiology 2020Quote: ... Wells were coated overnight with 1 μg of each ligand dissolved in bicarbonate buffer: collagen type I and IV (Sigma), elastin (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... followed by injection of the ligands (50 – 100 μg ml−1) in 10 mM sodium acetate pH 5.0 (Sigma, Germany) until the desired density was reached ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoassay plates were coated with 2.5 μg/ml recombinant angiotensin converting enzyme-2 (ACE2, Sigma Aldrich). Subsequently ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Immunology 2021Quote: ... 1 U/kg human insulin (Sigma) was given i.p ...
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bioRxiv - Immunology 2023Quote: ... 1 mg/mL human fibrinogen (Millipore), and 10 U/mL heparin (Sigma-Aldrich)).
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bioRxiv - Cell Biology 2023Quote: ... 1 ug/ml human insulin (Sigma), 10 nM hydrocortisone (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... 1:2000 human anti-CREST (Sigma) and donkey anti-human Dylight550 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1 % human albumin (Sigma). PBMC were thawed and rested overnight in X-Vivo-20 ...
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.