Labshake search
Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... human colorectal adenocarcinoma cell line Caco-2 were maintained in Dulbecco’s Modified Eagle’s Media (Sigma-Aldrich) supplemented with 10% FCS (Fetal calf serum ...
-
bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
-
bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
-
bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
-
bioRxiv - Biochemistry 2020Quote: ... Components for solubilisation buffer and ligands for both ThermoFRET stability assay and fluorescent ligand binding experiments were obtained from Sigma-Aldrich (Havervill, UK) and Anatrace (Ohio ...
-
bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
-
bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
-
bioRxiv - Biochemistry 2020Quote: ... His-PBP1B containing fractions were pooled and treated with 2 U/mL of thrombin (Novagen) for 20 h at 4 °C during dialysis against dialysis buffer I (25 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2024Quote: ... containing HI-2 at final concentration of 100 µM (or vehicle control DMSO, Sigma-Aldrich) was added to the cells ...
-
bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
-
bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... allowing intraluminal perfusion (10 ml.hr-1) of vehicle or test ligands using a syringe pump (Sigma-Aldrich).
-
bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
-
bioRxiv - Microbiology 2020Quote: Human promonocytic THP-1 cells were maintained in RPMI-1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
-
bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
-
bioRxiv - Neuroscience 2024Quote: ... After incubation with peroxidase-tagged secondary antibodies (1:2000 diluted, Sigma-Aldrich), membranes were revealed with an ECL-plus chemiluminescence western blot kit (Amershan-Pharmacia Biotech).
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... followed by HRP-tagged anti-rabbit secondary antibody (1:10,000, Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2021Quote: ... Apoptotic cell death was analyzed by staining with Annexin V/PI as per the manufacturer’s instructions (APOAF-20TST, Merck Millipore) and analyzed in BD LSR-Fortessa using FACS-Diva software (BD Biosciences) ...
-
bioRxiv - Cancer Biology 2021Quote: ... TUNEL Assay detecting apoptotic cell death were performed with the ApopTag Fluorescein in situ apoptosis detection kit (S7110, Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... aliquots were boiled in 100ºC water bath for 30 min and cell death was verified using 0.4% trypan blue solution (Millipore Sigma). Larvae were incubated in 37ºC post-injection and monitored daily for survival ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 μg of recombinant CDK1:CyclinB1 active complex (Millipore) in the presence or absence of 3 mM ATP ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 μg of recombinant CDK1:CyclinB1 active complex (Millipore) in the presence or absence of 3 mM ATP ...
-
bioRxiv - Cell Biology 2024Quote: ... The hexahistidine-tagged protein was expressed in Hi5 insect cells (Trichoplusia ni) grown in 1 liter of M3 medium (Sigma) supplemented with 10% fetal bovine serum (Wisent) ...
-
bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
-
bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
-
bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...
-
bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
-
bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
-
bioRxiv - Biochemistry 2021Quote: ... DSF thermal shift assays were developed to assess protein melting point (Tm) and thermal stability in the presence of putative small molecule ligands: 2-phosphoglycerate (2PG, Sigma-Aldrich 73885), phosphoserine (pSer ...
-
bioRxiv - Immunology 2021Quote: ... THP-1 cells were fixed with 2% paraformaldehyde (Sigma) and fluorescence of the cells was assessed by flow cytometry (BD ...
-
bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
-
bioRxiv - Microbiology 2019Quote: Non- and PUUV-Suo infected Mygla.REC.B cells were exogenously stimulated with TLR-3 ligand polyI:C (10 µg/ml, Sigma Aldrich) or Sendai virus (multiplicity of infection 1 ...
-
A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... 100 U/mL human interleukin-2 (Millipore Sigma; 11011456001), 2 ng/mL human interleukin-7 (Peptrotech ...
-
bioRxiv - Microbiology 2022Quote: ... and human thrombin (final concentration 2 U/ml; Sigma) in 50 μl Hank’s Balanced Salt Solution supplemented with 400 μg/ml aprotinin (Sigma) ...
-
bioRxiv - Immunology 2024Quote: ... and 100 U/ml human IL-2 (Sigma-Aldrich). After initial activation by plate-bound 0.05 µg of anti-CD3 (homemade ...
-
bioRxiv - Genomics 2020Quote: ... the cells were spun down and moved to “Phase 2” IMDM media containing 5% human serum (Sigma), 330µg/mL transferrin ...
-
bioRxiv - Physiology 2023Quote: ... Immortalized human LX-2 cells (MilliporeSigma, Burlington, VT, USA) were cultured in phenol-free DMEM (Sigma-Aldrich) containing 2% FBS (Gibco ...
-
bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
-
bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
-
bioRxiv - Plant Biology 2020Quote: ... Recombinant plasmid DNA was transferred to Escherichia coli strain Rosetta 2(DE3)pLysS (Novagen). At least four colonies for each experiment were sequenced to check for errors in the PCR ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
-
bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated with an anti-DIG FITC-tagged probe (Millipore cat #1120774191) diluted 1:200 in blocking buffer ...
-
bioRxiv - Bioengineering 2021Quote: ... Whole cell lysates were normalized to HRP-tagged Actin antibody (#A3854, Sigma Aldrich) and nuclear fractions to HRP-tagged Histone H3 (#12648 ...
-
bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
-
bioRxiv - Microbiology 2021Quote: ... these recombinant plasmids were introduced into Tuner (DE3) cells (Novagen). For co-expression of mxiK and spa33 ...
-
A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... The recombinant protein was produced in Rosetta (DE3) cells (Novagen) and purified by affinity chromatography using a GSTrapFF column ...