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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... PHM005 wild type and recombinant strains were grown in Marine Broth (MB) Difco 2216 (Sigma-Aldrich) or Marine Agar (MA ...
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bioRxiv - Microbiology 2020Quote: ... hAEC cultures were pretreated with recombinant universal type I IFN (100 or 10 IU/ml; Sigma) or recombinant IFN-λ3 (100 or 10 ng/ml 56 ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral particles encoding Flag-tagged human PDHA1 or shRNAs targeting mouse Pdha1 (TRCN0000041914/TRCN0000041914 (Sigma)) were produced in 293T packaging cells by transient transfection using Jet-PEI reagent (Ozyme) ...
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bioRxiv - Biochemistry 2020Quote: ... was destarched by incubation with amylase (0.2 mL, Termamyl 120 L, alpha-amylase from Bacillus licheniformis – Type XII-A, Sigma-Aldrich) in sodium phosphate buffer (125 mL ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... tris-HCl buffer and Porcine pancreatic lipase type II were purchased from Sigma [USA].
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bioRxiv - Neuroscience 2019Quote: ... HRP solution was prepared by dissolving 125mg of HRP type II (Sigma, P8250) in 2.5ml PBS ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 mg/mL (adult) or 2mg/mL (fetal) Collagenase Type II (Sigma). The digestion mixture was strained (70 μM) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... for the rat Liver-Chip we used a mixture of collagen type IV (Sigma-Aldrich, St. Louis, MO, USA) and fibronectin (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... on collagen coated plastic (50 μg/cm2 of rat tail collagen Type IV (SERVA, 47256.01) in 0.02N acetic acid(Sigma; 537020)) ...
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bioRxiv - Biophysics 2023Quote: ... The suspended cells in DMEM were mixed with rat tail-derived type I collagen in 0.1% acetic acid and 2X DMEM (Sigma). After neutralizing the pH with sodium hydroxide ...
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bioRxiv - Cell Biology 2022Quote: ... the heterogeneous cell population was resuspended in EC culture medium and seeded in collagen type I (125-50, Sigma)-coated plates ...
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bioRxiv - Microbiology 2023Quote: ... Prior to seeding HepG2 cells, wells and coverslips were surface-coated with collagen (type I, rat tail) (Sigma-Aldrich) in a 50 µg/mL solution in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Cell Biology 2020Quote: ... alpha tubulin (Sigma); p-myosin light chain (Ser19 and Thr18/Ser19) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal against alpha-tubulin (1:1000; b5-1-2, Sigma, T6074). Actin was stained using 100 nM of phalloidin-iFluor488 for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Plates were coated 1:1 with collagen (Cat nb C8919, Sigma) and laminin (Cat nb 23017-015 ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteases were obtained from the following vendors: human cathepsin L (Millipore Sigma, Athens Research and Technology ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng/ml cholera toxin and 10 μg/ml recombinant human insulin (Sigma). MCF10A I-PpoI cells and MCF10A AsiSI cells were previously described (Harding et al. ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 0.5 mg/mL recombinant human albumin (Sigma-Aldrich, St. Louis, MO), 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... and 200 nM of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... After 16 h 50 ng/ml of recombinant human (rh)TGFA (Sigma-Aldrich) and/or Afatinib (5 µM ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of sample or standard (Recombinant human IFN-α, IFNA: Millipore, IF007) were added ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng per well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-sarcomeric alpha-actinin (clone EA53, Sigma-Aldrich, 1:1000), P-Smad1/5/8 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used were alpha-tubulin 1:500 (Sigma T9026), MEK1/2 1:1000 (Cell Signaling 8727s) ...
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bioRxiv - Cell Biology 2021Quote: ... monoclonal mouse anti-Alpha Tubulin (T8203, Sigma; dilution: 1:1000), monoclonal mouse anti-Puromycin (EQ0001 ...
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bioRxiv - Cell Biology 2020Quote: ... alpha-smooth muscle actin (1:150, Cat nb. A5228, Sigma) or NG2 (rabbit ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-alpha-Tubulin (aTUB) (Sigma-Aldrich #T5168, 1:4000). HRP conjugated secondary antibodies against mouse proteins were obtained from ThermoFisher and used at 1:5000 dilution.
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Expansion of apical extracellular matrix underlies the morphogenesis of a recently evolved structurebioRxiv - Developmental Biology 2019Quote: ... mouse anti-alpha tubulin (acetylated) 1:500 (T6793, Sigma-Aldrich), rat anti-Ecadherin 1:500 (DCAD2 ...
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bioRxiv - Molecular Biology 2019Quote: ... and alpha-cardiac actin (Sigma, cat # A9357, 1:300 dilution) diluted in the blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-lys40 acetylated alpha tubulin (1/500, Sigma, #T6793), rat anti-alpha tubulin (1/200 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-alpha-tubulin B-5-1-2 (Sigma, T5168), mouse anti-alpha-tubulin TAT-1 (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-alpha tubulin (Merck-SIGMA, clone B-5-1-2) was used as a loading control at 1:10000.
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bioRxiv - Neuroscience 2022Quote: ... and alpha tubulin (α-tubulin; Sigma-Aldrich; T5168; 1:10,000).
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bioRxiv - Physiology 2023Quote: ... and mouse anti-alpha-Tubulin (Sigma #T9026, diluted 1:4000) in Odyssey blocking buffer + 0.2% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-alpha-tubulin (Sigma-Aldrich, T6074, 1:10,000) were used ...
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bioRxiv - Microbiology 2023Quote: ... virus was treated with alpha-1-antitrypsin (Sigma-Aldrich, SRP6312) at the concentration noted ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-sarcomeric alpha actinin (mouse, Sigma-Aldrich A7811; 1/200) anti Twist1 (mouse 1/300 Novusbio NBP2) ...
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bioRxiv - Immunology 2024Quote: ... mouse monoclonal alpha-tubulin antibody (#T5168, Sigma-Aldrich, 1:10.000), goat anti-mouse IgG ...
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bioRxiv - Biophysics 2023Quote: ... rabbit anti-alpha-catenin (Sigma Aldrich C-2081, 1:200), and rabbit anti-desmoplakin (abcam ab71690 ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies used were mouse alpha tubulin (1:500, Sigma T9026) and goat anti-mouse AlexaFluor 488 (1:500 ...