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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... and collagen IV (Millipore AB756P 1:100). Antigen retrieval was performed to visualize Casp3 ...
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bioRxiv - Developmental Biology 2019Quote: ... goat-α-collagen IV (1:800, Millipore), goat-α-Gli3 (1:200 ...
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bioRxiv - Genetics 2021Quote: ... pH 7.4) before being incubated with primary antibodies (Anti-Telo-Collagen Type I, A1, ABT256, Sigma-Aldrich; Anti-Fibronectin-1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded at a density of 2500 cells per cm2 onto Collagen Type I (Sigma-Aldrich) coated plates in Skeletal Muscle Induction Media (5% horse serum ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated on plastic coated with 0.06 µg/cm2 calf skin collagen type I (Sigma, UK) and were cultured to confluency in complete endothelial cell growth medium MV2 (PromoCell GmbH ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were seeded in rat tail type I collagen (100 µg/mL, Sigma Aldrich, St. Louis, MO)-coated flasks and maintained in modified Endothelial Growth Media 2 ...
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bioRxiv - Physiology 2022Quote: ... Cells were plated onto glass coverslips coated with collagen (type I rat tail, Sigma; 10 μg/cm2) and maintained in a humidified atmosphere of 5% CO2 and 95% air at 37 °C in Williams’ Medium E supplemented with penicillin ...
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Neuroscience 2023Quote: ... anti-rabbit TRITC tagged (1:200, Sigma) and anti-mouse FITC tagged (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human epidermal growth factor (20 ng/mL, Sigma-Aldrich). Cell authentication by Ipatimup’s Cell Lines Bank ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblast Growth Factor basic human recombinant animal-free (GF003AF; Sigma Aldrich); hEGF Epidermal Growth Factor Human (E9644 ...
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bioRxiv - Genomics 2022Quote: ... Recombinant human insulin (0.75 IU insulin/kg BW) (Sigma-Aldrich, I9278) was then administered intraperitoneally ...
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bioRxiv - Immunology 2019Quote: ... and recombinant human interleukin-2 (rhIL-2; 25U/ml; Sigma-Aldrich). To mimic a transient stimulation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... baseline blood glucose was taken before recombinant human insulin (Sigma, USA) (0.5 U/kg – 1 U/kg ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human MMP-3 (SRP7783) was purchased from Sigma Aldrich (Germany).
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bioRxiv - Cell Biology 2022Quote: ... 20 ng/ml recombinant human epidermal growth factor (hEGF, #E9644, Sigma), 0.25 ng/mL hydrocortisone (#H0888 ...
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bioRxiv - Bioengineering 2022Quote: ... and recombinant insulin human (≥ 98%) were all purchased from Sigma-Aldrich. Water (LC-MS grade) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant human Hepatocyte Growth Factor (HGF) was obtained from Sigma Aldrich Israel ...
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bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... were seeded at 15,000 cells.cm−2 in 96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (100ng.mL−1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell ...
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bioRxiv - Microbiology 2019Quote: ... or anti-His (1:1000 Sigma) overnight at 4 °C and anti-mouse secondary (1:5000) ...
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bioRxiv - Microbiology 2021Quote: ... α-His (Sigma, cat# H1029, 1: 3,000), α-ICDH (1 ...
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bioRxiv - Microbiology 2020Quote: ... α-His(Sigma, cat# H1029, 1: 10,000), α-HA (Santa Cruz ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-HIS (Sigma H1029 1:2,500) or anti-MBP (New England Biolabs E8032 ...
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bioRxiv - Cell Biology 2023Quote: ... The neutralized collagen was dropped in Lab-Tek II chambered cover glass (Sigma-Aldrich, Z734853) for 15 µL per well ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with purified collagen I from human placenta (Sigma), gelatin from bovine skin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with purified collagen I from human placenta (Sigma), gelatin from bovine skin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Microbiology 2019Quote: ... 100 µl of a THP-1 cell suspension (106 cells/mL) in 20 mg/mL collagen from rat tail type I (Sigma Aldrich, St. Louis, USA) were spread in the basolateral surface of inverted inserts ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Neuroscience 2021Quote: ... permebilized and incubated with 5U/ml DNase I type II (SIGMA #D4527) for 15 min at RT ...
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bioRxiv - Microbiology 2022Quote: Type II mucin from porcine stomach (Sigma-Aldrich, Saint-Louis, Missouri, USA) and sodium alginate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... fragments were dissociated with 0.5 mg/mL dispase type II (Sigma-Aldrich), 1.5 – 5 mg/mL collagenase type II (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... resuspended in 3000 U/mL type II collagenase (Sigma-Aldrich, Cat#C6885) digestion buffer supplemented with 100 U/mL DNase I (Worthington ...
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bioRxiv - Cell Biology 2020Quote: ... the cell medium was replaced with DMEM containing 0.2% FBS and 300ng/mL IGF-1 (human recombinant, Sigma) for 3 hours ...
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bioRxiv - Cell Biology 2022Quote: ... unsorted cells were cultured for 60 hours on bovine collagen type I (Sigma, Cat# C2124; 2.5 μg/cm2) coated flasks in a humidified incubator (5% CO2 ...
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bioRxiv - Genomics 2021Quote: ... cells were dissociated into single-cell suspensions and plated onto type IV collagen coated (60 ug/mL, Sigma) 96-well plates at a density of 25,000 cells per well ...
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bioRxiv - Cell Biology 2021Quote: Platelet subpopulations (2 × 106) were incubated on Horm collagen type I (100μg/ml; Takeda) or fibrinogen (100μg/ml, Sigma) coated coverslips for 90 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Cell Biology 2023Quote: ... 4⨉106 cells were seeded into each well of a collagen (type I from calf skin - Millipore Sigma)-coated 12-well plate and grown at 37 ℃ for 24 h in a CO2-jacketed incubator by which they reached 100% confluence ...
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bioRxiv - Biophysics 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Biochemistry 2021Quote: ... After removal of the His-tagged MBP by Re-IMAC the unbound material was concentrated in 50 kDa centrifugal concentrators (Millipore) and subjected to SEC on a Superdex 200 Increase column (equilibrated 150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... These were prepared by preincubating the His-tagged PilC with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 1:1.5:1.5 (by weight ...
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...