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Citations for Millipore Sigma :
301 - 350 of 647 citations for Recombinant Hirudin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and supplemented with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore). Cultured mESCs at 60% confluency were washed with PBS twice before the preparation of total lysate ...
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bioRxiv - Developmental Biology 2022Quote: ... FGF treatments were done using recombinant Fibroblast Growth Factor-Basic (F3685, Sigma-Aldrich) concentrations were 300 ng/mL ...
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bioRxiv - Cell Biology 2022Quote: All experiments were performed using the BDNF mature recombinant (GF029; Merck Millipore, USA) or the C-ter truncated form of BDNF (BDNFΔC-ter) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA (10 µg) was incubated with recombinant RNAse-free DNase I (Sigma) in 50 µl-reaction tubes at 37° C for 20 minutes ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were DNase I digested (DNase I recombinant, Rnase-free, Millipore Sigma), cleaned up (RNA Clean & Concentrator-5 ...
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bioRxiv - Cell Biology 2022Quote: ... After 16 h 50 ng/ml of recombinant human (rh)TGFA (Sigma-Aldrich) and/or Afatinib (5 µM ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of sample or standard (Recombinant human IFN-α, IFNA: Millipore, IF007) were added ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant proteins were produced and purified on glutathione-agarose column (G4510, Sigma) for GST-tagged protein or on TALON-Cobalt beads column (635507 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng per well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Neuroscience 2023Quote: ... Michigan) and 0.6 TIU/mL of recombinant aprotinin was added (Sigma; Burlington, MA). For saliva ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days.
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bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Biochemistry 2023Quote: ... periplasmic expression of the recombinant GFP-nanobody in BL21(DE3) Rosetta cells (Novagen) was induced with 0.1 mM IPTG for 16 hours at 16°C ...
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated with recombinant Cholera toxin B (CTxB)-FITC (Sigma Aldrich C1655) for 1 h to stain for lipid rafts and then with an anti-TLR4 antibody (Abcam ab22048 ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant plasmids were transformed into BL21 (DE3) or BL21 (DE3) Tuner cells (Novagen) and were incubated in 1L LB in 2L conical flasks at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich, St. Louis, MO) to promote differentiation into M2 macrophages ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant plasmid DNA was transferred to Escherichia coli strain Rosetta 2(DE3)pLysS (Novagen). At least four colonies for each experiment were sequenced to check for errors in the PCR ...
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bioRxiv - Cell Biology 2019Quote: Morphogenesis was induced by the addition of recombinant human HGF (product H1404, Sigma-Aldrich) and conditioned media from NIH/3T3 cells (product CRL-1658 ...
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bioRxiv - Neuroscience 2021Quote: ... Peprotech) and recombinant rat IFNγ (reconstituted in pH 8 sodium phosphate [10mM; Sigma-Aldrich] were diluted to the required concentrations (LTα 1μg + IFNγ 75ng per injection ...
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bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of recombinant PRDX6 was induced with 1mM isopropyl β-d-thiogalactopyranoside (IPTG, Sigma). The culture was incubated for 20h at 18°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant protein was then concentrated using centrifugal filter units (Amicon Ultra-4, Millipore) and dialyzed using Slide-A-Lyzer dialysis cassette into storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human recombinant MAO-A (hMAO-A) and MAO-B (hMAO-B) enzymes (Sigma-Aldrich) were diluted in 50 mM phosphate buffer (final protein amount ...
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bioRxiv - Evolutionary Biology 2020Quote: ... ephyrae were placed in ASW supplemented with 500 nM human recombinant insulin (Sigma I0908). Insulin was refreshed weekly ...
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bioRxiv - Cell Biology 2020Quote: ... HMWHA or BCM− was pre-incubated with 300 IU/mL recombinant HYAL2 (Sigma-Aldrich) for 30 min prior to addition to co-cultures.
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Molecular Biology 2020Quote: ... Digestion was performed overnight at 37 °C using recombinant dimethylated SOLu-trypsin (Sigma-Aldrich) with a trypsin:protein ratio 1:20 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Purified recombinant S proteins were concentrated using Amicon filtration devices (EMD Millipore, Billerica, MA), buffer-exchanged into PBS and analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was purified by affinity chromatography using anti-FLAG affinity resin (Sigma). The purified protein was precipitated by 20% trichloroacetic acid ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RNase R (30 pmols) or Bovine Serum Albumin (BSA, Sigma-Aldrich, MO, USA) were alone or combined with Csm5 WT or Csm5Δ46 (90 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: We concentrated recombinant EGFP fusion proteins using Amicon Ultra centrifugal filters (10K MWCO, Millipore) to an appropriate protein concentration in 250 mM NaCl salt buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µM AMP (Serva) and active recombinant AMPK (16 ng) (Sigma-Aldrich; 14-840) in a total volume of 30 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, R and D) and Purmorphamine (1.5 μM, Sigma). Plates were then incubated at 37°C at 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Developmental Biology 2023Quote: ... This basal medium was completed with 5ng/ml recombinant mouse LIF (Sigma ref LIF2050), 3µM CHIR99021 (Sigma ref SML1046-5MG ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Cell Biology 2020Quote: ... The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma, SRP0360) were carried out as described previously (Alvarez-Rodrigo et al. ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
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bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...