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Citations for Millipore Sigma :
251 - 300 of 647 citations for Recombinant Hirudin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 3 μM CHIR99021 (Cayman) and 1,000 U/ml recombinant mouse LIF (Millipore)39 ...
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bioRxiv - Microbiology 2021Quote: ... and 40 ng/mL bFGF (basic recombinant human fibroblast growth factor; Sigma) in flasks coated first with 50 µg/mL poly-L-ornithine (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and supplied with 1000 U/ml recombinant leukemia inhibitory factor (LIF, Millipore). 293T cells were cultured in DMEM supplemented with 10% FCS.
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bioRxiv - Biochemistry 2021Quote: ... and supplied with 1000 U/ml recombinant leukemia inhibitory factor (LIF, Millipore). ESCs were cultured on plates which were pre-coated with 0.1% gelatin ...
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bioRxiv - Genetics 2020Quote: ... 10 ng/μL recombinant fibroblast growth factor basic (βFGF; Sigma-Aldrich, #F0291), 10 μg/μL Heparin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 40 ng/ml recombinant human long-range IGF-1 (Sigma-Aldrich, USA), 5 ng/ml recombinant IL-6/soluble IL-6R fusion protein ((Fischer et al. ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 1000 units/ml ESGRO Recombinant Mouse LIF protein (Millipore #ESG1107), 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: Limited proteolysis experiments were conducted with recombinant human cathepsin S (Milipore-Sigma) diluted in phosphate-citrate buffer at pH 5.6 and 37 °C(21) ...
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bioRxiv - Synthetic Biology 2021Quote: Recombinant hACE2 with a C-terminal deca-histidine tag (SAE0064, Sigma-Aldrich) was resuspended in PBS to a final concentration of 50 ng/μL ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were digested overnight at 37 °C with recombinant trypsin (Trypzean, Sigma) (enzyme ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human holo-lactoferrin (iron-saturated) (L1294) was purchased from Sigma-Aldrich. Aliquots were prepared by resuspending in PBS at a concentration of 1 mg/ml and were stored at −20 °C ...
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bioRxiv - Immunology 2021Quote: ... Recombinant Wnt5A (Cat no.- 10UG 645-WN-010 R & D, GF146 Millipore) and recombinant Wnt3A (Cat no.- 10UG 5036-WN-010 R & D ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... eluted in water and aliquoted for later use with recombinant Cas9 (Sigma). Oligos used for sgRNA production are listed in Extended Data Table 4.
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... media supplemented with 1000U/mL ESGRO Recombinant Mouse LIF (Millipore Sigma, ESG1107), 2 mM GlutaMAX (Gibco ...
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bioRxiv - Immunology 2023Quote: ... then tested against recombinant proteins coated in Dulbecco’s PBS (DPBS, Sigma-Aldrich) overnight onto 96-well Immunlon-2 plates (VWR Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore).
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was digested by adding 10 U DNase I recombinant (Sigma) and incubating for one additional hour ...
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bioRxiv - Biochemistry 2023Quote: ... They were incubated with recombinant ULK1 proteins (Sigma, #SRP5096-10UG, Darmstadt, Germany) in the kinase buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 10 μg/μl recombinant mouse leukemia inhibitory factor (LIF; Sigma-Aldrich) in a 5% CO2 incubator at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG ...
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bioRxiv - Developmental Biology 2021Quote: ... All additional recombinant genes were cloned into pFLAG-CMVTM-6a expression vector (Sigma) unless otherwise specified ...
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bioRxiv - Genetics 2020Quote: ... 200 µg/ml Proteinase K (Proteinase K, recombinant, PCR Grade, 3115879001, Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteases were obtained from the following vendors: human cathepsin L (Millipore Sigma, Athens Research and Technology ...
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bioRxiv - Molecular Biology 2021Quote: ... and 103 U/mL ESGRO recombinant mouse leukemia inhibitory factor (LIF) (Millipore; ESG1107) in an humidified cell incubator with 5% CO2 at 37°C.
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bioRxiv - Biophysics 2020Quote: The recombinant MdmX (18-111 aa) was cloned into pET46Ek/LIC vector (Novagen) and expressed in E.coli BL21 (DE3 ...
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bioRxiv - Biophysics 2020Quote: The recombinant human Mdm2 (1-125 aa) were cloned into pET11a vector (Novagen) and expressed in E ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng/ml cholera toxin and 10 μg/ml recombinant human insulin (Sigma). MCF10A I-PpoI cells and MCF10A AsiSI cells were previously described (Harding et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant SBF or MBF complex was purified with anti-FLAG resin (M2, Sigma) from insect cells co-infected with FLAGSwi4-Swi6 or FLAGMbpl-Swi6 baculovirus constructs ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich) for derivation of a standard curve ...
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bioRxiv - Cancer Biology 2020Quote: ... JSH23 (Cat# 481408) and Recombinant human IGF-1 (Cat# GF138) purchased from Millipore; JQ1 (Cat# M2167 ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 0.5 mg/mL recombinant human albumin (Sigma-Aldrich, St. Louis, MO), 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant PRDX6 was eluted with lysis buffer supplemented with 250 mM imidazole (Sigma). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... recombinant GM-CSF (10ng/ml; Peprotec) and β-estradiol (1 μM; Sigma-Aldrich). To differentiate into macrophages ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10 ng/ml of human rIGF1 (recombinant Insulin growth factor-1) (Sigma, USA), 10 ng/ml amphotericin B ...
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected in each matrix 25 μg of Recombinant Sema3C-Fc (Sigma-Aldrich) in a concentration of 50 μg/ml in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Purified recombinant active TGF-β1 was purchased from Sigma Aldrich (St. Louis, MO). Immunofluorescence imaging was conducted using the following primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... or C1-27 was added to 0.5µg of recombinant GSTO1 (Sigma, GS75-100ug) in PBS in 12uL total volume and incubated in a thermocycler at 37°C for 20 mins in a competitive CMFDA binding assay described previously (28) ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1 mM β-mercaptoethanol and 1,000 U/ml recombinant leukaemia inhibitory factor (Millipore). For differentiation ESCs were resuspended at 1×104 cells/ml in DMEM (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... and recombinant Wnt3A (Cat no.- 10UG 5036-WN-010 R & D, GF154 Millipore) were purchased from R & D Systems and Millipore ...
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bioRxiv - Immunology 2022Quote: ... the culture medium was supplemented with 2.5ug/mL recombinant mouse EGF (Sigma-Aldrich) 16 hours before harvesting.
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bioRxiv - Microbiology 2022Quote: ... and 200 nM of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) for 1 h at 37 °C ...