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Citations for Millipore Sigma :
701 - 750 of 8860 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Microbiology 2020Quote: Female BALB/c mice have been injected intraperitoneally with 700 μg recombinant His-tagged TGT protein that was emulsified in complete Freund’s adjuvant (Sigma-Aldrich). Two weeks later ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...
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bioRxiv - Plant Biology 2019Quote: ... using a VP-ITC microcalorimeter (MicroCal) as described [34] after extensive dialysis of the purified recombinant protein in Chelex 100 (Sigma) to remove contaminating divalent cations ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cell Biology 2019Quote: ... Immune sera were generated by immunization of 6 weeks-old female NMRI mice (Charles Liver Laboratories) via subcutaneous injection of 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks with 50 µg of recombinant protein ...
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bioRxiv - Biophysics 2021Quote: Catalytic activity was evaluated in vitro using 20 pmol of purified recombinant proteins in a 200 µl reaction buffer supplemented with 20 mM p-nitrophenyl phosphate (Sigma) as substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Isotopically labelled proteins were expressed using Escherichia coli BL21 DE3 cells in M9 minimal media supplemented with 15NH4Cl (Sigma-Aldrich ISOTEC). Chaetomium thermophilum Naa5082-289 containing a C-terminal His-tag was expressed using E ...
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bioRxiv - Molecular Biology 2019Quote: ... coli as a fusion protein with the ketosteroid isomerase (KSI) using the pET31 plasmid (Kuliopulos et al., 1994) (Novagen/EMD Biosciences). KSI fusion proteins are concentrated in inclusion bodies in E ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml EGF (Sigma, E 4127) and 100 μl LIF(Millipore ...
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bioRxiv - Biochemistry 2020Quote: Anti-Japanese encephalitis E (mouse monoclonal, Millipore), influenza A H9N2 HA (mouse monoclonal ...
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bioRxiv - Molecular Biology 2020Quote: Gamma secretase inhibitor Compound E (Millipore Sigma) was dissolved in DMSO and diluted 1:1000 in RPMI media to a final concentration of 1 μM ...
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bioRxiv - Systems Biology 2021Quote: ... 10 μM E-64 (Sigma-Aldrich # 324890), 1.45 μM pepstatin (Sigma-Aldrich # P5318) ...
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bioRxiv - Microbiology 2020Quote: ... The cathepsin inhibitor E-64d (Sigma-Aldrich), the serine protease inhibitor camostat mesylate (Abcam) ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... Rabbit anti-CENP-E (C7488, Sigma Aldrich), diluted 1:100 ...
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bioRxiv - Biochemistry 2021Quote: ... Ergosterol obtained from Sigma-Aldrich (E-6625), cholesterol ester ...
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bioRxiv - Developmental Biology 2019Quote: ... 1.5 mg/mL ProNase E (Sigma P6911), 62.5 U/mL DNAse (Applichem ...
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bioRxiv - Biochemistry 2020Quote: ... pepstatin and E-64 (Sigma Aldrich, USA). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Culture medium was Williams’ Media E (Sigma) supplemented with nicotinamide (12 mmol/L) ...
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bioRxiv - Developmental Biology 2022Quote: ... 1x β-mercaptoethanol (Millipore, ES-007-E), 1µM PD98059 (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 μM E-64 (Sigma, E3132) for protease inhibition ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 μg/ml E-64 (Sigma-Aldrich), 5 mM EDTA (Calbiochem) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20-hydroxyecdysone (20-E) (H5142; Sigma Aldrich) was resuspended to an initial stock concentration of 10 mM in ethanol.
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bioRxiv - Immunology 2024Quote: ... and stained with H&E (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... and 3.4 mM vitamin E (Sigma-Aldrich) (45) ...
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bioRxiv - Cancer Biology 2021Quote: ... Kinase assays were performed as described in (20) using 200ng of purified EPHA2 recombinant protein (OriGen Technologies) or indicated concentration of purified Enolase (Sigma Aldrich), in the absence or presence of about 50ng of purified Src-GFP (or Src3A-GFP as indicated ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Cell Biology 2020Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg of protein from each sample was incubated for two hours with purified Multidsk2 recombinant protein bound to glutathione agarose magnetic beads (Sigma G0924). After incubation beads were washed four times with D-buffer and resuspended in NuPage LDS sample buffer (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Cancer Biology 2022Quote: ... 7μL of each dilution was then used in SDS-PAGE alongside a dilution series of recombinant GST protein (Sigma-Aldrich #SRP5348) from 5ng-200ng ...
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bioRxiv - Microbiology 2023Quote: ... mixture of BEI-EMCV strain SING-M105 or recombinant VP1 protein prepared according or BEI-EMCV with Complete Freund’s adjuvant (Sigma-Aldrich, USA) was injected intramuscularly into five BALB/c mice at the concentration of 50 μg of antigen per mouse ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Bioengineering 2023Quote: ... The hybridized RNAs were then mixed with 25 µL of 30 µM (5 mg/mL) recombinant SpCas9 protein (Sigma-Aldrich #Cas9PROT) for 30 minutes at 4 C ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... coli (Novagen and Supplementary file 5) were transformed with pMH-HS-Sumo-dOCRL168-509 vector (KanR ...
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bioRxiv - Microbiology 2021Quote: ... coli strain Rosetta2 (DE3) pLysS (Novagen). For each purification procedure ...