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Citations for Millipore Sigma :
51 - 100 of 1654 citations for Real Time Thermal Cycler Thermocycler Accessories since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Olympus BioLevers (k = 60 pN/nm) were calibrated using the thermal noise method and incubated in 2.5 mg/ml Concanavalin A (C5275, Sigma) for 1 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... First-strand synthesis was then performed by incubation at 25 °C for 10 min and 50 °C for 50 min on a PCR cycler with 7.6 μl of the following mix: 0.2 μg actinomycin (Sigma A1410), 13.15 mM DTT (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (4 times for 20 s each time) and lysozyme (1 mg/ml; Sigma) in 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (4 times for 20 s each time) and lysozyme (1 mg/ml; Sigma) in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (4 times for 20 s each time) and lysozyme (1 mg/ml; Sigma) in 50 mM Na Phosphate ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed by sonication (4 times for 20 s each time) and lysozyme (1 mg/ml; Sigma) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Plant Biology 2023Quote: ... and vortexed 10 times for 30 s each time in the presence of glass beads (Sigma-Aldrich, G8772). Total cell extract was separated by centrifugation (15000g for 20 min at 4°C) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by sonication (4 times for 20 s each time) and lysozyme (1 mg/ml; Sigma) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Immunology 2019Quote: ... 3 times with 0.05% Tween 20 (Sigma) in PBS (PBS-T) ...
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bioRxiv - Genetics 2020Quote: ... rinsed 3 times with PBS1X (Sigma, D8662) and blocked with 5% normal donkey serum (NDS ...
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bioRxiv - Genetics 2023Quote: ... washed 3 times in PBS 1X (Sigma) with 0,1% Tween20 (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... First-strand synthesis was then performed by incubation at 25 °C for 10 min and 50 °C for 50 min on a PCR cycler with 7.6 μl of the following mix: 0.2 μg actinomycin D (Sigma-Aldrich, A1410), 13.15 mM DTT (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (4 times for 20 s each time) and lysozyme (1 mg/ml; Sigma-Aldrich) in 50 mM Na phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... DSF thermal shift assays were developed to assess protein melting point (Tm) and thermal stability in the presence of putative small molecule ligands: 2-phosphoglycerate (2PG, Sigma-Aldrich 73885), phosphoserine (pSer ...
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bioRxiv - Neuroscience 2021Quote: ... Lastly cells were washed three times with 0.2% Triton X-100 solution for about 5 minutes each time and mounted in Fluoromount (Sigma, F4680) with 0.1% DAPI in it ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were washed an additional three time with TBS-T and one time with TBS before 3 minute exposure with ECL HRP substrate (Millipore) and imaged on a BioRad ChemiDoc system ...
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bioRxiv - Cell Biology 2023Quote: ... at which time cultures were washed three times with pre-warmed HBSS containing 100 ug/mL heparin (Sigma-Aldrich H3393) to remove any transiently adhered ...
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bioRxiv - Genetics 2023Quote: ... ∼20mg pulverised frozen liver tissue was dounced ten times with pestle A and ten times with pestle B (Sigma # 8938) in 1ml hypotonic lysis buffer solution B containing 10 mg/ml BSA (Sigma # 126609) ...
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bioRxiv - Molecular Biology 2021Quote: ... At indicated time (hpi) 500 uM 4SU (Sigma) was added to cell culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... three times with LiCl wash buffers (Millipore Sigma), and two times with TE buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS three times and trypsinized (Sigma). Cell pellets were additionally washed three times with PBS before fixation ...
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bioRxiv - Cell Biology 2020Quote: ... washed three times with PBS (Sigma Aldrich, USA) and blocked for 45 minutes with 0,1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times in pre-warmed PBS (Sigma), twice in pre-warmed DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... washed four times in ethanol (Sigma-Aldrich, 459836) and coated onto PVC tubing (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: A time-course assay employing trypsin (Sigma Aldrich) was devised to investigate the degradation of the adenylate kinases ...
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bioRxiv - Neuroscience 2024Quote: ... washed three times with PBS (1x; Sigma; P5493) and blocked for 1 h with blocking buffer (BB ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 2 times with 1X PBS and 1 time with MilliQ before mounting on microscope slides using Mowiol (10% Mowiol (Millipore, 475904), 0.1% diazabicyclo(2,2,2)-octane ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were washed 10 times with RIPA buffer then 5 times with 100 mM ammonium hydrogen carbonate (AMBIC) (Sigma-Aldrich) solution to remove salts and detergents ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 2 times with 1X PBS and 1 time with MilliQ before mounting them on microscope slides using Mowiol (10% Mowiol (Millipore, 475904), 0.1% diazabicyclo(2,2,2)-octane ...
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bioRxiv - Genomics 2022Quote: ... The tissue was homogenized with 10 times of slow douncing using a loose pestle and 15 times of douncing using a tight pestle (D9063, Sigma-Aldrich). The homogenate was filtered through a 40-μm cell strainer (22363547 ...
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bioRxiv - Biochemistry 2021Quote: ... The effect of metabolites on the thermal stability of SdGA was measured using 0.1 mM acarbose (Sigma-Aldrich cat. n° 56180-94-0), 1 mM CaCl2 or 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was homogenized in a glass Dounce tissue grinder (25 times with pestle A, 25 times with pestle B; Sigma, Cat#D8938) in 2mL ice-cold EZ Nuclei Lysis Buffer (Sigma ...
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bioRxiv - Biophysics 2019Quote: ... (4) four times BRB40 buffer (40 mM Pipes [Sigma] ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed three times with RPMI media (Sigma) prior to addition of melan A-specific T cells (healthy donor-derived ...
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bioRxiv - Microbiology 2022Quote: ... washed two times with PBS pH 7.2 (Sigma-Aldrich) and resuspended in 2 ml of PBS ...
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bioRxiv - Cell Biology 2022Quote: ... At indicated times auxin (3-indolo acetic acid, Sigma) was added at a final concentration of 1-2.5 mM (table S4) ...
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bioRxiv - Microbiology 2021Quote: ... Fixed cells were washed three times with DPBS (Sigma) + 0.02% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Two times concentrated drug solution including tamoxifen (Sigma-Aldrich), paclitaxel (Selleckchem ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then rinsing three times with milli-Q water (Millipore) and lastly soaking in 70% ethanol for 2 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 10 times using Amicon centrifugal filter units (Merck Millipore) with a 100 kDa cut-off to a final volume of 5 mL ...
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bioRxiv - Microbiology 2022Quote: ... 10 times using Amicon centrifugal filter units (Merck Millipore) with a 100 kDa cut-off to a final volume of 20 mL ...
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bioRxiv - Microbiology 2023Quote: ... three times using 100 kDa amicon filters (Merck, Millipore) and centrifuging at 3’000 × g for 6 min ...
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bioRxiv - Cell Biology 2023Quote: ... this time using 100 mM NaN3 (Sigma-Aldrich, S8032) to allow the lineup of animals ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The membranes were washed three times with PBS (5 min each time) and were then incubated with secondary peroxidase-conjugated antibody (1:15,000; Sigma-Aldrich, St. Louis, MO, USA). The antibody-bound proteins were detected using Pierce ECL western blotting substrate (Thermo Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed with TBST buffer (three times, 7 minutes each) and incubated with anti-mouse-HRP or anti-rabbit-HRP (Sigma-Aldrich; 20,000 times dilution) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... cells were washed three times with Tyrode’s Salts (Sigma-Aldrich), stained for 40 min with 2 μM Fura Red™ ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed four times before TMB (Sigma Aldrich) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... and flown multiple times over FLAG-M2 affinity beads (Sigma) which were pre-equilibrated in lysis buffer and packed in a gravity flow column ...
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bioRxiv - Neuroscience 2020Quote: ... and concentrated three times with a Centriprep centrifugal filter (Millipore) at 1,220 × g for 5 min each and with an Amicon Ultra centrifugal filter (Millipore ...