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Citations for Millipore Sigma :
1 - 50 of 1654 citations for Real Time Thermal Cycler Thermocycler Accessories since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The qPCR reaction was performed on a qTOWER3 real-time thermal cycler (Analytik Jena) with KAPA SYBR FAST master mix (Sigma-Aldrich). Gene expression was monitored using gene-specific primers ...
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bioRxiv - Genomics 2019Quote: ... Real time PCR was carried out in a Stratagene MX3005P thermocycler using SYBR Green Jump Start Taq Ready Mix (Sigma Aldrich, USA) for quantifying amplification results ...
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bioRxiv - Plant Biology 2021Quote: ... All PCR reactions were performed in a Bio-Rad T100 thermal-cycler using Phusion High-Fidelity DNA Polymerase (Thermo-fisher) or KOD Hot Start DNA Polymerase (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: Custom primers for real-time PCR were purchased from Sigma-Aldrich, Germany (GAPDH ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time qPCR was performed using SYBR Green JumpStart Taq ReadyMix (Sigma) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Real-time qPCR was performed with the following probes from Sigma Aldrich: LOX fw ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and all real-time PCR reagents and primers were obtained from Sigma-Aldrich. All organ culture and cell culture media components were obtained from Sigma-Aldrich and Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Real-Time qPCR was performed using the Fast SYBR Green Master Mix (Sigma) in a QuantStudio 5 Real-Time-PCR-Cycler (Thermofisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed using Jumpstart SYBR green mastermix (Sigma-Aldrich) on a Rotorgene 6000 (Corbett Research) ...
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bioRxiv - Cancer Biology 2023Quote: cDNA was diluted 10-50 times and quantified by real-time PCR with LightCycler® 480 SYBR Green I Master (SIGMA). The RPL11 ...
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bioRxiv - Bioengineering 2020Quote: ... The nucleotide sequences for RT-PCR and real-time PCR primers (synthesized by Sigma-Aldrich) are shown in Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... fixed accessory glands were permeabilised for 6 x 10 min in PBST (1 X PBS, 0.3% Triton X-100 [Sigma-Aldrich]), blocked for 30 min in PBSTG (PBST ...
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bioRxiv - Microbiology 2021Quote: ... in a thermocycler with desalted oligonucleotide primers (Sigma-Aldrich, UK) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: For real-time quantitative reverse transcription PCR (RT-qPCR) total cellular RNA was extracted with Trizol (Sigma) or QiAmp (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time detection of miRNA expression was performed by using MystiCq miRNA qPCR Assay Primer (Sigma-Aldrich), using Universal Mouse Reference RNA and U6 as reference for mouse and human cells ...
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bioRxiv - Microbiology 2022Quote: Quantitative real-time PCR (rt-qPCR) was carried out using the KAPA SYBR® FAST Mix (Sigma) on a LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Physiology 2022Quote: ... Each real-time PCR reaction mix was prepared with SYBR Green JumpStart Taq ReadyMix (Sigma, Oakville, Canada) and forward and reverse primer mix (3 µM ...
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed on Bio-Rad CFX96 real time (qPCR) system using predesigned KiCqstart primers (Sigma) and TBB green (#RR-208A ...
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bioRxiv - Cell Biology 2021Quote: ... and Sox2 cDNA were amplified by real-time qPCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich) and a 7500 Real-Time PCR Detection System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... Each real-time qPCR reaction mix was prepared with SYBR Green JumpStart Taq ReadyMix (Sigma, Oakville, ON, Canada) and forward and reverse primer mix (3 µM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and real-time quantitative reverse transcriptase PCR analysis (qRT-PCR) was performed using SYBR Green® reagent (Sigma Aldrich) as previously described (46) ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR using cDNA was performed using KAPA SYBR fast qPCR master mix kit (Sigma Aldrich, USA) and Applied Biosystems StepOne Plus Real-Time PCR system ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PRC using cDNA was performed using KAPA SYBR fast qPCR master mix kit (Sigma Aldrich, USA) and Applied Biosystems StepOne Plus Real-Time PCR system ...
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bioRxiv - Cell Biology 2020Quote: Intracellular NO was measured in real time using the NO-specific fluorescence probe DAF-FM DA solution (Sigma Aldrich). DAF-FM DA diffuses freely across the membrane ...
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bioRxiv - Genetics 2022Quote: ... Real-time PCRs were done in a 10 μl volume using the Absolute quantitative PCR SYBR Green mix (Sigma) in a 96-well plate ...
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bioRxiv - Molecular Biology 2020Quote: ... Real time quantitative PCR was performed with KAPA SYBR FAST qPCR Master Mix (Sigma-Aldrich - Merck, UK, cat no KK4602) according to manufacturer’s instructions in a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: Q-PCR was performed on a Roche Lightcycler using standard reverse transcriptase kit and SYBR Green Real-Time PCR Master Mixes (SIGMA).
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Real-time RT-PCR (TaqMan) assay-specific primers and probes for ZIKV were designed by Sigma-Aldrich (St Louis, MO) following the protocol developed by the Molecular Diagnostics and Research Laboratory Centers for Disease Control and Prevention (CDC) ...
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bioRxiv - Microbiology 2020Quote: Thermal denaturation of citrate synthase (0.25 μM final concentration, Sigma) was achieved by incubation at 45°C ...
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bioRxiv - Plant Biology 2020Quote: ... qRT–PCR was performed with the Bio-Rad CFX384 Real-Time PCR system using the SYBR Green Jumpstart Taq ReadyMix (S4438-100RXN; Sigma-Aldrich) with the following PCR conditions ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded on 96-well PCR plate and run in CFX real-time PCR System (Bio rad) using KiCStart SYBR Green qPCR ReadyMix with ROX as recommended by the manufacturer (Sigma-Aldrich: KCQS02). Three technical replicates were performed for each sample together with no-template control (NTC ...
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bioRxiv - Cancer Biology 2021Quote: ... The real-time PCR analysis was also performed with the Complementary DNAs using JumpStart Taq DNA polymerase (Sigma-Aldrich, St. Louis, MO) and SYBR green nucleic acid stain (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... thermal inhibitor 2,6-Di-tert-butyl- 4-methylphenol (BHT, Sigma-Aldrich), and photoinitiator Irgacure-651 (I-651 ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with an Applied Biosystems 7500 Real-Time PCR system using KiCqStart®SYBR®Green qPCR ReadyMix™ (Millipore-Sigma). Gene expression was normalized to the internal control 18S rRNA ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed in the Applied Biosystems 7500 FAST real-time PCR system using SYBR Green JumpStart Taq ReadyMix (Sigma-Aldrich, St Louis, US). Transcript levels of target genes were determined via the 2-ΔΔCt method (Livak & Schmittgen ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with an Applied Biosystems 7500 Real-Time PCR system using KiCqStart®SYBR®Green qPCR ReadyMix™ (Millipore-Sigma). Gene expression was normalized to the internal control 18S rRNA ...
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bioRxiv - Cell Biology 2021Quote: ... calibrated using thermal noise method and coated with 2.5 mg/ml Concanavalin A (C5275, Sigma) for 1 hour at 30°C ...
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bioRxiv - Biochemistry 2019Quote: FeNPs were synthesized by thermal decomposition of Iron acetylacetonate (Sigma-Aldrich, St Louis, MO, USA). In brief ...
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bioRxiv - Biochemistry 2020Quote: ... PaYbbN or EcYbbN to inhibit the thermal aggregation (43 °C) of citrate synthase (SIGMA #C3260) was compared with the protein without treatment ...
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bioRxiv - Plant Biology 2024Quote: ... on 500 ng of RNA and qPCR was performed on 5x diluted cDNA in a BioRad CFX opus 384 thermocycler with SYBR Green (Sigma). Relative expression values were calculated using the reference gene MpaEF1.
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bioRxiv - Systems Biology 2023Quote: ... 50 μM and 100 μM compound for exactly 5 minutes at 25 °C in a thermocycler (Biometra TRIO) before proteinase K (Proteinase K, Tritirachium album, 10 mg, Sigma Aldrich) digestion ...
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bioRxiv - Systems Biology 2023Quote: ... for exactly 5 minutes at 25 °C in a thermocycler (Biometra TRIO) before proteinase K (Proteinase K, Tritirachium album, 10 mg, Sigma Aldrich) digestion ...
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bioRxiv - Bioengineering 2020Quote: ... Non-thermal treatments were conducted by incubating engineered cells at indicated concentrations of CoCl2 (Sigma #232696-5G) or CdCl2 (Sigma #202908) ...
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bioRxiv - Biophysics 2022Quote: For the initial thermal aggregation assays 550 nM citrate synthase from porcine heart (Sigma-Aldrich C3260-5KU) in a 1:2 protein:DNA strand concentration (1.1 µM DNA ...
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bioRxiv - Biochemistry 2020Quote: ... was determined in thermal shift experiments (30,31) For determination fluorescent protein stain SYPRO® Orange (5000x, Sigma-Aldrich) and CFX Connect Real-Time PCR Detection system (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... and washed 3 times in 1x PBS and 2 times in 2x SSC buffer (Sigma-Aldrich) for five min each ...
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bioRxiv - Genetics 2021Quote: ... washed two times in M9 and one final time in M9 including 1 mM cycloheximide (Sigma). Samples were then washed with lysis buffer prepared in nuclease free water (10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Cantilevers were calibrated using the thermal noise method and coated with 2.5 mg/ml Concanavalin A (#C5275, Sigma Aldrich). Prior to the measurements ...
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bioRxiv - Cell Biology 2019Quote: ... Olympus BioLevers (k = 60 pN/nm) were calibrated using the thermal noise method and incubated in 2.5 mg/ml Concanavalin A (C5275, Sigma) for 1 h at room temperature ...