Labshake search
Citations for Millipore Sigma :
401 - 450 of 466 citations for Radio Flow Detectors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... Neutrophil viability was measured using Muse Annexin V and Dead Cell Assay Kit on a Muse Cell Analyzer mini-flow cytometer (EMD Millipore).
-
bioRxiv - Biochemistry 2020Quote: ... samples were made up to 200 µL with TBS+4% BSA and analyzed using a Guava benchtop mini-flow cytometer (EMD Millipore). In all experiments ...
-
bioRxiv - Bioengineering 2019Quote: ... in BRDM using a syringe pump at a flow rate of 30 µl/h or with 0.5 mg/ml and 2 mg/ml Gentamicin (Sigma-Aldrich, USA) for 6 days ...
-
bioRxiv - Biochemistry 2020Quote: ... samples were made up to 200 µL with TBS+4% BSA and analyzed using a Guava benchtop mini-flow cytometer (EMD Millipore). Virus titer was quantified as an average of (total cell number×%gp64 staining×virus dilution factor)/20 µL (volume of inoculum).
-
bioRxiv - Immunology 2020Quote: In the reconstitution experiments the percentage of live GFP+/propidium iodide− cells was determined by flow cytometry by labelling transfected cells with 0.5 μg/ml of propidium iodide (Sigma-Aldrich, #537059).
-
bioRxiv - Biochemistry 2022Quote: ... The clarified harvest was concentrated approximately ten-fold by flat sheet tangential flow filtration (TFF) filters with a molecular cut-off 500 kDa (EMD Millipore). The flat sheet filters had a combined surface area of 4 m2 ...
-
bioRxiv - Biophysics 2022Quote: ... Cells were then fixed with 4% paraformaldehyde for 15 min at room temperature and measured using flow cytometry (Guava easyCyte, EMD Millipore).
-
bioRxiv - Biochemistry 2023Quote: ... To enrich any remaining phosphopeptides not captured using the TiO2 column the flow-throughs were passed over PHOS-Select IMAC (P9740, Sigma Aldrich) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular cytokine production was analyzed using flow cytometry after 4 hours of polyclonal restimulation with 100 ng/mL phorbol myristate acetate (PMA, P8139, Sigma-Aldrich) and 1 µg/mL ionomycin (I0634 ...
-
bioRxiv - Biochemistry 2023Quote: ... was concentrated from the flow-through of the second passage through the His-trap column using an Amicon® Ultra Centrifugal Filter (Millipore) with a 30 kDa cut-off ...
-
bioRxiv - Microbiology 2023Quote: ... The DNA content of the cells was analyzed using the ImageStreamX MarkII Quantitative imaging analysis for flow cytometry system (Merck Millipore, Germany). About 20,000 cells were collected for each sample and the data of the single cells were analyzed with the Flowjo software.
-
bioRxiv - Developmental Biology 2023Quote: ... cells were imaged (Ex/Em – 561 and 650 nm for MtPhagy dye and 488/550 nm for Lysosomal dye) using an Amnis ImagestreamX MKII imaging flow cytometer (EMD Millipore). Appropriate unstained cell and single-color cell controls were used to generate a compensation matrix which was used to convert raw image files to compensated image files and the co-localization wizard from IDEAS® v6.0 (Amnis ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were treated for 1-2 hours prior to and for the duration of flow experiments with 1 µM S1P receptor antagonist fingolimod (FTY720) (stock 1 mM in DMSO, Sigma #SML0700). FTY720 experiments were performed with Protocol 1 in flow media supplemented with 0.3% BSA ...
-
bioRxiv - Neuroscience 2024Quote: ... Through the recording chamber there was a continuous flow of carbongenated (95% O2/5% CO2) artificial cerebrospinal fluid (aCSF) (124 mM NaCl (Sigma; S5886), 1.25 mM NaH2PO4 (Sigma ...
-
bioRxiv - Biochemistry 2024Quote: ... The diluted mixture was concentrated to 25 mL using an Amicon centrifugal filter and run continuously (0.5 mL/min) over a 5 mL HiTrap® SP Fast Flow FPLC column (Millipore Sigma) for 2-3 hours to maximize protein capture ...
-
bioRxiv - Microbiology 2023Quote: ... and the flow-through containing cleaved protein was collected and concentrated to 2 mL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
-
bioRxiv - Molecular Biology 2023Quote: ... The column flow-through and washes containing CpxA31-163 were pooled and concentrated using a 3,000 MWCO spin concentrator (Millipore, Fisher Scientific). As a final purification step ...
-
bioRxiv - Bioengineering 2023Quote: We used 1x106 cells per sample for flow cytometry staining in a polypropylene 96-well round bottom plate (Sigma, cat#P6866). All staining steps were performed in 100 μL volume in the dark at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... determined by BCA assay were precleared for 1 hour with Protein G Sepharose 4 Fast Flow beads (GE17-0168-01, Millipore Sigma) at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Electrophysiological recordings were performed at 25°C in an immersion chamber under a constant flow of carbogenated aCSF supplemented with picrotoxin (100 µM, Sigma-Aldrich) to block γ-amino-butyric acid type-A receptors ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
-
bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
-
bioRxiv - Immunology 2021Quote: ... Samples were washed and resuspended in flow cytometry buffer/FACS Buffer (PBS, 1% bovine serum albumin [Sigma-Aldrich], 2mM EDTA [Life Technologies]) for acquisition on a BD Fortessa SORP ...
-
bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
-
bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were then washed with PBS and resuspended with 80 µL of staining buffer for imaging flow cytometry (ImageStream®X Mark II, EMD Millipore) at the UCLA Flow Cytometry Core Facility of Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research ...
-
bioRxiv - Cancer Biology 2020Quote: ... at Thomas Jefferson University Flow Cytometry Shared Resource was used to measure fragmented (subG1) apoptotic DNA with propidium iodide (Sigma-Aldrich P4170), Annexin V/7AAD (BD Pharmingen 559763) ...
-
bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
-
bioRxiv - Microbiology 2019Quote: ... and staining (1:4,000 dilution of commercial SYBR Green I stock) aliquots for 18 h in the dark at room temperature and counting by flow cytometry (EMD-Millipore Guava EasyCyte), as described previously (54) ...
-
bioRxiv - Cell Biology 2020Quote: ... Emulsions were formed by flow-focusing the collagen/cell solution and oil (fluorocarbon oil FC-40 (Sigma Aldrich, St. Louis, MO, USA)) with 2% FluoroSurfactant (RAN Biotechnologies ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Perifusate fractions were collected at the flow rate of 3ml/min and insulin was measured using radioimmunoassay kit (Millipore, Cat #: RI-13K). Insulin secretion were normalized to IEQ.
-
bioRxiv - Molecular Biology 2020Quote: Confluent monolayers of HAEC grown on 6-well plate were exposed to unidirectional PSS (6, 12 and 24 hours) using a modified flow device.10 Neutralized MCDB-131 medium (Sigma Aldrich, MO) containing 7.5% sodium bicarbonate solution ...
-
bioRxiv - Neuroscience 2021Quote: ... standardized to 1 mg/ml) was precleared with 50 μl Protein G Sepharose 4 Fast Flow (Millipore Sigma Cat#GE17-0618-01) or μ rProtein A Sepharose Fast Flow (Millipore Sigma Cat#GE17-1279-01 ...
-
bioRxiv - Cell Biology 2019Quote: ... The percentage of COX-2 positive cells was quantified on 5.103 dissociated UC-hMSCs using a Guava® easyCyte Flow Cytometer (Merck Millipore, Guyancourt, France). The results were compared to the fluorescent intensity distribution obtained by image analysis.
-
bioRxiv - Microbiology 2020Quote: ... the bacteria were inoculated into the same medium (0.2% v/v) and the cell density was monitored using a Guava EasyCyte flow cytometer before harvesting (Merck Millipore, Billerica, MA, USA). Cell pellets were harvested from 10 ml culture by centrifugation which were stored at −80°C prior to lipid extraction.
-
bioRxiv - Biophysics 2021Quote: ... The glass coverslips used to assemble the flow cell (#1 thickness, 24×60 mm MenzelGlazer, Germany) were washed by sonication in a solution of Hellmanex III (Sigma-Aldrich, Germany) diluted in demineralized water (1% v/v) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and stained in the dark at room temperature for 10 min before data acquisition and data analysis on a Guava easyCyte™ flow cytometer (EMD Millipore). Conditions were assayed in triplicate and experiments were performed at least twice.
-
bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
-
bioRxiv - Immunology 2023Quote: ... Capillary blood flow was visualized in some experiments through intravenous injection with 18 mg/kg of dextran-rhodamine 70 kD (Sigma-Aldrich; R9379). Cell tracking analysis was performed by re-visiting the same area of the dermis in separate imaging experiments through using inherent landmarks of the skin to navigate back to the original region ...
-
bioRxiv - Physiology 2023Quote: ... MEFs were selected with 3 μg/mL of puromycin and transduction efficiency for mCherry was assessed by flow cytometry using Guava EasyCyte™ (Millipore, USA). A transduction efficiency of 95% or greater was achieved.
-
bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).
-
bioRxiv - Cancer Biology 2024Quote: ... This was followed by flow cytometric detection after staining of bead-bound sEVs with fluorescent anti-CD63 phycoerythrin (PE) (Sigma-Aldrich, USA).
-
bioRxiv - Immunology 2023Quote: ... flow rate: 30 μL/min) of decreasing concentrations of the PTPRZ1 CA (Cusabio Technology LLC, CSB-YP019068HU) or CAII (Sigma Aldrich, C2522) proteins (2-fold cascade dilutions from the starting concentration of 800 nM) ...
-
bioRxiv - Microbiology 2020Quote: ... 29 The sample was dried under nitrogen gas flow and purified with a 3 kDa Amicon Ultra centrifugal filter (Millipore, Temecula, CA, USA) to remove low molecular weight impurities ...
-
bioRxiv - Neuroscience 2021Quote: ... The supernatant obtained at this step was filtered through 0.45-μm filters and injected into a sample loop connected to HiPrep Q Fast Flow 16/10 (Sigma-Aldrich, GE28-936-543). The supernatant was injected at 2 mL/min and eluted using buffer B (40 mM Tris-HCl ...
-
bioRxiv - Biophysics 2019Quote: Cells sampled from the same non-aggregated populations of FAMSeqAPEGDPPE-modified cells as those analyzed by confocal microscopy and post-digestion fluorescence spectroscopy were sampled with a flow cytometer (5000 cells, Guava Easy Cyte Mini, Millipore, Billerica, MA, USA). Flow cytometry-based measurement of number of cells per μL of suspension were used to determine the number of cells treated with Benzonase for fluorescence spectroscopy analysis of liberated FAM.
-
bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
-
bioRxiv - Evolutionary Biology 2020Quote: Cell counts were obtained by flux cytometry in a Biorad flux cytometry Guava easyCyte 14HT flow cytometer (EMD MILLIPORE Ltd, Oakville, ON, Canada). Counts were obtained from 5,000 events of serial dilutions of the co-cultures in a 96 deep-well plate ...
-
O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The flow-through was concentrated using an Amicon 15mL-volume centrifugal concentrator unit equipped with a 3kDa MWCO membrane (EMD-Millipore, Burlington, MA, USA) before being injected onto a Superdex 75 16/600 HiLoad column (Cytiva ...
-
bioRxiv - Microbiology 2021Quote: ... to remove the protease and the flow through concentrated using a 30,000 molecular weight cut-off centrifugal filter unit (EMD Millipore, Billerica, MA, USA).