Labshake search
Citations for Millipore Sigma :
301 - 350 of 466 citations for Radio Flow Detectors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Samples were analysed using a Guava® easyCyte™ 12HT flow cytometer (Luminex, Yokohama, Japan; previously Millipore, Darmstadt, Germany). Yeast concentrations were adjusted to inoculate sterile Shiraz juice at a final rate of 5 × 106 live cells mL-1.
-
bioRxiv - Microbiology 2021Quote: ... cells were flow sorted for dTomato positive cells and maintained in RPMI media supplemented with 0.2µg/mL puromycin (Sigma, #P8833) selection ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then diluted to 200 μl with cold PBS and acquired using a GUAVA flow cytometer (Merck Millipore). Propidium Iodide (PI,Sigma ...
-
bioRxiv - Cell Biology 2019Quote: ... Flow cells were first incubated with 30 μl of 1:100 dilution of monoclonal anti-β-Tubulin antibody (Sigma) for 10 minutes in a humidified chamber ...
-
bioRxiv - Plant Biology 2021Quote: ... For Co-IPs with MED10B-MEC-ProtA we used IgG Sepharose® 6 Fast Flow (Sigma, GE17-0969-01) beads and increased washing steps (1xIP buffer ...
-
bioRxiv - Cancer Biology 2020Quote: ... Fixed cells were treated with RNase at 1 mg/ml final concentration for 30 min at 37°C or overnight at 4°C before adding 5 mg/ml PI and analyzed with Guava Easycyte 8HT flow cytometer equipped with Guava Soft 2.1 (Millipore).
-
bioRxiv - Biophysics 2020Quote: The flow-through fraction from the column was collected and concentrated using an Amicon Ultra 50K filter (Merck Millipore) and then loaded onto a Superdex 200 10/300 size-exclusion chromatography (SEC ...
-
bioRxiv - Cell Biology 2020Quote: ... counted and centrifuged and washed twice in flow buffer −0.5% mass/volume (w/v) bovine serum albumin (BSA) in PBS (both from Sigma). A total of 50 000 cells from each sample were transferred into a sterile polystyrene test tube (BD Bioscience ...
-
bioRxiv - Neuroscience 2020Quote: ... The following beads were used in immunoprecipitations: protein A-Sepharose 4 B Fast Flow from Staphylococcus aureus (P9424, Sigma), Protein G Sepharose 4 Fast Flow (17-0618-01 ...
-
bioRxiv - Microbiology 2022Quote: ... the DNA content was analysed using the ImageStreamX MarkII Quantitative imaging analysis for flow cytometry system (Merck Millipore, Germany), which was calibrated with non-labelled beads with a diameter of 2 μm ...
-
bioRxiv - Biochemistry 2022Quote: ... with a flow rate of 100 μl.min−1 at 25°C with different concentrations of various analytes: PGA (81325, Sigma), citrus pectin with DM 24-30% (P9311 ...
-
bioRxiv - Neuroscience 2023Quote: ... Flow cells were primed by flushing 55 ul of a solution of 5% Pluronic F-127 (Sigma-Aldrich, P2443) and then incubated for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... Sequential fraction 3.5 until 5.5 mL was collected from the flow-through and further concentrated using the Amicon Ultra 0.5 mL 10 kDa filter (Millipore). To isolate EV-associated lipids ...
-
bioRxiv - Immunology 2022Quote: ... Cells were acquired the following day on a Fortessa 1770 flow cytometer following the addition of counting beads (Sigma) and analyzed using FlowJo software (version 10).
-
bioRxiv - Systems Biology 2022Quote: ... Zenoaq) and stored in liquid nitrogen for flow cytometry analyses or seeded onto fibronectin-coated (20 µg/ml, Millipore) 48-well dishes at a concentration of 2.5 x 105 cells per cm2 in DMEM/F12 (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... in- house flow-chambers were constructed using slides and cover slips treated with 0.1 % poly-L- lysine (Sigma-Aldrich) [83] ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were either taken for flow cytometry analysis or cryopreserved using a 1:9 ratio of DMSO (Millipore Sigma) and FBS (Thermo Fisher Scientific) ...
-
bioRxiv - Biophysics 2023Quote: For immunoprecipitation nProtein A Sepharose 4 Fast Flow (17528001, Cytiva) or anti-FLAG® M2 Magnetic Beads (M8823, Sigma) were used in the proportion of 1 μL of beads per 20 μg of total protein lysate ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was sequentially filtered through a tangential flow filtration cassette with 0.45 µm pore size (PVDF membrane; Pellicon XL 50, Millipore) and concentrated on a 1,000 kDa MWCO tangential flow filtration cassette (regenerated cellulose membrane ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were diluted ten-fold in fresh CT medium and assayed with a Guava EasyCyteHT flow cytometer (Millipore). Cell concentrations were determined by gating on red fluorescence ...
-
bioRxiv - Biochemistry 2023Quote: ... MWRA flow-through protein solution was concentrated to ∼15 mL using a 30 kDa MWCO centrifugal filter (EMD Millipore), ensuring not to concentrate to where solution became yellow/cloudy and viscous ...
-
bioRxiv - Immunology 2023Quote: ... The online digestion and trapping were performed for 4 minutes using a flow of 50 µL/min 0.1 % FA (Sigma), pH 2.5 ...
-
bioRxiv - Neuroscience 2023Quote: ... the ability to reverse the nonresponsive phenotype for astrocytes in 0% BSA was tested by >1 hour incubation prior to and for the duration of flow experiments with 1 µM S1P (stock 0.2 mM in 1:5 methanol:water, Sigma #S9666) in 0% BSA flow media using Protocol 1 ...
-
bioRxiv - Physiology 2023Quote: ... and a backward flow of phosphate buffered saline (PBS) followed by 10% formaldehyde (Sigma-Aldrich, St. Louis, MO, USA) was introduced using a micro syringe pump (MSD-1D ...
-
bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
-
bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... Macrophages were characterized by cytospin and flow cytometry under basal conditions and following LPS (Sigma-Aldrich St. Louis, MO) stimulation (100 ng/nL for 72 hours ...
-
bioRxiv - Biophysics 2021Quote: ... The flow-through was concentrated to 1 mL using a 10 kDa Amicon Ultra-15 Centrifugal Filter (Millipore Sigma, UFC901096) before placing over a 5 mL Q column (Cytiva ...
-
bioRxiv - Microbiology 2020Quote: ... Infected cultures were then either trypsinized for detection of GFP-positive cells and/or dextran-positive cells by flow cytometry on a Guava EasyCyte 6/2L bench cytometer (Millipore), or fixed with 4% paraformaldehyde and analyzed by fluorescence microscopy after labeling with antibodies specific for UIS4 (Sicgen ...
-
bioRxiv - Microbiology 2022Quote: ... Snow was melted over a period of 12 hours and concentrated using Pellicon tangential flow filters (Millipore, Burlington, MA, USA) to a final volume of ∼ 10 mL ...
-
bioRxiv - Neuroscience 2021Quote: ... collected by centrifugation and superoxide anion production was determined by flow cytometry with Muse® Oxidative Stress Assay Kit (Millipore), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: The concentrated elute was dialyzed with buffer BC-50 (BC buffer with 50 mM NaCl and 5% glycerol) and loaded on a 5 mL HiTrap Q Fast Flow anion exchange chromatography column (Sigma) using an ÄKTATM Pure FPLC (GE Healthcare) ...
-
bioRxiv - Genomics 2020Quote: ... was then added at 1:20,000 dilution and samples were run on a Guava easyCyte flow cytometer (Millipore, Billerica, MA). 20,000 events were captured for each time point ...
-
bioRxiv - Microbiology 2019Quote: ... Viral size particles in the filtrate were concentrated to ~500 mL (viral concentrate) using tangential flow ultrafiltration with a 30kDa MW prep-scale Spiral Wound TFF-6 cartridge (Millipore) (53).
-
bioRxiv - Immunology 2019Quote: ... The cells were washed twice with 1× PBS and resuspended in 1× PBS and analyzed using Guava easyCyte 12HT flow cytometer (Millipore) and FlowJo software (Tree Star).
-
bioRxiv - Bioengineering 2020Quote: ... and cells were fed with a continuous flow at 1 Psi of HS media containing 2% glucose and 0.001% Fluorescence Brighter 28 (Sigma-Aldrich). After 24 hours ...
-
bioRxiv - Microbiology 2021Quote: ... The resin was loaded into a column and the flow through containing S-tagged gyrase A was further purified using an S-protein agarose (Sigma) by elution with 20 mM Tris pH 7.5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 150 000 cells in 100 μL of flow cytometry buffer were stained with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) for 15 min at 37°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2.0×105 cells were collected and stained in 100 μl of Guava Nexin reagent for 20min then collected on Guava system flow cytometer (Millipore).
-
bioRxiv - Microbiology 2020Quote: ... The supernatant was concentrated by tangential flow filtration (TFF) using a Pellicon® 2 mini-cassette system (Millipore, MO, USA) with a 30 kDa pore size ...
-
bioRxiv - Microbiology 2021Quote: ... The rest of the water was filtered through 10-μm plankton and concentrated using tangential flow filtration (TFF) system (Millipore) with 0.22-μm polycarbonate membrane filters ...
-
bioRxiv - Immunology 2021Quote: ... Data was obtained through the Amnis ImageStreamX Mark II imaging flow cytometer and INSPIRE software (EMD Millipore, Billerica, MA, USA). The accompanying IDEAS software (EMD Millipore ...
-
bioRxiv - Cancer Biology 2020Quote: The presence of phosphorylated H2A.X was measured by flow cytometry according to the manufacturer’s instructions (Upstate Cell Signaling, Millipore Sigma). Briefly ...
-
bioRxiv - Cancer Biology 2020Quote: ... at a flow rate of 150 μL/min on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μm, EMD Millipore) at 35°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was purified by collecting the flow-through from a second Ni-Sepharose column and concentrated using Amicon spin filters (Millipore). Protein was stored at -20 □.
-
bioRxiv - Microbiology 2022Quote: ... Excision of the GFP cassette in blood stage parasites was monitored by flow cytometry using a Guava EasyCyte 6/2L bench cytometer equipped with 488 nm and 532 nm lasers (Millipore) to detect GFP and mCherry ...
-
bioRxiv - Cancer Biology 2022Quote: ... while BM chimerism was analysed 20 weeks after transplantation. Chimerism was assessed by flow cytometry (using the antibodies detailed in TableS7.d. 7AAD (Sigma) was used for dead cell exclusion ...
-
bioRxiv - Biophysics 2021Quote: ... The fractions of the flow-through containing MSP protein were pooled together and concentrated with a 10 kDa Amicon Ultra-filter (Millipore) to 100-200 μM ...
-
bioRxiv - Microbiology 2021Quote: ... the DNA content or cell sizes were analyzed using the ImageStreamX MarkII Quantitative imaging analysis flow cytometry (Merck Millipore, Germany), which was calibrated with non-labeled beads with a diameter of 2 µm ...
-
bioRxiv - Cell Biology 2020Quote: ... Microtubules were immobilized in the flow cell by means of an anti-tubulin antibody (Sigma Aldrich, T7816, 10 µg/ml). Subsequently ...