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Citations for Millipore Sigma :
651 - 700 of 4429 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... A total of 1 µl of locus specific PCR product was used for indexing PCR using KOD Hot Start DNA Polymerase (EMD-Millipore, 71086-31) and TruSeq i5 and i7 indexing primers (Illumina ...
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bioRxiv - Cell Biology 2019Quote: PCR products were ligated into the p3XFLAGCMV10 (Sigma-Aldrich) and pEGFP-C1 (Clontech ...
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bioRxiv - Cell Biology 2020Quote: ... PCR-amplified DNA fragments were inserted into pET21b (Novagen) without adding a tag using NheI and XhoI restriction sites ...
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bioRxiv - Genomics 2019Quote: ... and 1 µl of 10× PCR Buffer (Sigma Aldrich) with 0.25 units of JumpStart Taq DNA Polymerase (Sigma Aldrich ...
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bioRxiv - Genomics 2020Quote: ... PCR reactions were prepared with 1.0M Betaine (Sigma-Aldrich), and were heated to 80°C before adding Taq Polymerase (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was ligated into pET28a(+) plasmid (Novagen), which was linearized with Nhe I and BamH I ...
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bioRxiv - Cancer Biology 2020Quote: ... Regular Mycoplasma testing was performed using MycoLookOut PCR (Sigma). OVSAHO ...
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bioRxiv - Biochemistry 2020Quote: ... PCR products were cloned in the pET28b vector (Novagen) linearized with BamHI and NotI according to the sequence and ligation-independent cloning (SLIC ...
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bioRxiv - Microbiology 2020Quote: ... KiCqStart SYBR green primers for qRT-PCR (Sigma-Aldrich) were specific for MCM2 ...
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bioRxiv - Cell Biology 2021Quote: ... Oligonucleotides for PCR amplification were obtained from Sigma Aldrich. For each construct the relevant open reading frame was completely verified by Sanger sequencing (GATC/Eurofins Scientific) ...
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bioRxiv - Microbiology 2022Quote: Expand High Fidelity PCR System (Sigma-Aldrich, catalog #: 11759078001).
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bioRxiv - Cancer Biology 2022Quote: ... confirmed by the use of Mycoplasma PCR Detection (Sigma) were regularly passaged to maintain exponential growth and used from early passages (<10 passages after thawing) ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR primers were synthesized by Sigma Aldrich (S2 Table), longer DNA fragments and genes were ordered from GeneScript (S3 Table) ...
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bioRxiv - Cancer Biology 2020Quote: ... or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... PCR primers and oligos were purchased from Sigma-Aldrich. Restriction enzymes were purchased from New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Amplicons were purified using a PCR cleanup kit (Sigma) and quantified using a Qubit 2.0 fluorometer (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The purified PCR product and pET-28a(+) (Novagen, Germany) were digested with BamH I and Xho I (NEB ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were then cloned in the pET15b (Novagen) plasmid modified compatible with GatewayTM cloning strategy ...
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bioRxiv - Cancer Biology 2022Quote: ... Regular mycoplasma testing was performed using MycoLookOut PCR (Sigma). When were they last tested? HGSOC lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... using RedExtract-n-Amp tissue PCR kit (Sigma-Aldrich). The G2 progeny of genome-edited males were then raised until homozygous stocks were established ...
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bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
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bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
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bioRxiv - Microbiology 2023Quote: ... Colony PCR was performed using REDtaq Ready Mix (Sigma) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were amplified by PCR using KOD Xtreme polymerase (Novagen) using primers (Table S5 ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were run in 2% agarose (SIGMA) gels and visualised under UV light.
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bioRxiv - Cell Biology 2024Quote: ... All PCR was performed using KOD polymerase (EMD Millipore). To generate pPM09 ...
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bioRxiv - Immunology 2021Quote: ... macrophages were detached from the plate using Accutase solution (Sigma-Aldrich) at 37°C for 15min followed by manual shaking and repeated pipetting ...
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bioRxiv - Immunology 2021Quote: ... or Corning® Costar® Ultra-Low Attachment plates (Sigma-Aldrich), which are coated with a proprietary hydrogel designed to minimise cell attachment ...
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bioRxiv - Microbiology 2021Quote: ... Co-inoculated plates were sealed with 3M tape ([Millipore] 0.5 cm) to reduce growth differences between inner and peripheric wells ...
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bioRxiv - Microbiology 2019Quote: High-binding 96-well plates (Cat# CLS3361; Millipore Sigma, Burlington, MA) were either coated directly with 500 ng/well of recombinant DENV2 16681 E protein (Cat# DENV2-ENV-500 ...
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bioRxiv - Neuroscience 2020Quote: ... 24 well plates were first coated with Poly-L-lysine (Sigma). Around 4×105 cells/well were plated onto 24-well plates containing a mix of ADAM17 variants with eiger fused to AP plasmids and transfection reagent (Fugene) ...
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bioRxiv - Developmental Biology 2020Quote: ... Plates were then stained for alkaline phosphatase (Sigma, 86 R-1KT) and rescanned with transmitted light ...
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bioRxiv - Cell Biology 2022Quote: ... WTC11 cells were dissociated from the plate using accutase (Sigma, #A6964) and passaged every 2 days ...
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bioRxiv - Immunology 2021Quote: ... Finally plates were washed and developed with BCIP®/NBT (Sigma).
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bioRxiv - Immunology 2021Quote: ... plates were developed with 1 mg/mL OPD (Sigma; P3804-100TAB) in citrate buffer (PBS with 0.05 M NaH2PO4 and 0.02 M Citric acid ...
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bioRxiv - Immunology 2021Quote: ... ELISpot plates were coated with native Insulin (Sigma-Aldrich, Cat. 91077C), anti-IgM (Mabtech ...
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bioRxiv - Neuroscience 2019Quote: ... slices were placed on Millicell culture plate inserts (Millipore, Burlington, MA) in OptiMEM (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... the plate was sealed with a gas-permeable membrane (Sigma-Aldrich) and placed on a plate shaker set to 600 rpm to achieve proper mixing of the suspension ...
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bioRxiv - Microbiology 2019Quote: ... Green plates (43) contained methyl blue (Sigma-Aldrich, St. Louis, MO) instead of aniline blue ...
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bioRxiv - Genetics 2019Quote: ... using replica platers for 48-well or 96-well plates (Sigma), with or without various drugs as indicated in figure legends.
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bioRxiv - Biochemistry 2019Quote: ... BMDMs were derived on untreated plastic plates (BD Falcon, Sigma-Aldrich) by culturing in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... cells were replicated onto plates containing 100 µg/ml G418 (Sigma). Colonies were screened by colony PCRs to confirm correct integrations at both 5’ and 3’ ends ...
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bioRxiv - Cell Biology 2019Quote: Animals were grown on NGM plates containing either colchicine (Sigma-Aldrich) or paclitaxel (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or avidin coated wells (Avidin, Sigma-Aldrich on MaxiSorp plates, Sigma). Monoclonal antibodies were isolated from the synthetic human scFv antibody library ETH-2-Gold [19] by two rounds of biopanning as previously described [39 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or avidin coated wells (Avidin, Sigma-Aldrich on MaxiSorp plates, Sigma). Monoclonal antibodies were isolated from the synthetic human scFv antibody library ETH-2-Gold [19] by two rounds of biopanning as previously described [39 ...
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bioRxiv - Cancer Biology 2021Quote: ... filtered through a 0.22 μm hydrophilic PVDF membrane filter plate (Millipore). 70 μl were used for depletion as described above followed by Filter-Aided Sample Preparation (FASP)32 and 30 ul for the neat plasma comparison ...
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bioRxiv - Microbiology 2021Quote: ... using 50ul per 15cm plate X-tremeGENE 9 (Sigma-Aldrich, 8724121001). Media was changed at 24 hr post-transfection ...
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bioRxiv - Biophysics 2021Quote: ... 6-well Lab-Tek plates were coated with Collagen (Sigma-Aldrich) in 0.1 M Acetic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then covered with UV sterilised breathable membranes (Sigma Aldrich) and incubated at 37°C for 7 days ...