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Citations for Millipore Sigma :
501 - 550 of 1716 citations for Mayaro virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... whole cell lysates were incubated with the M2 FLAG magnetic beads (Sigma) for at least 6 h in cold room ...
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bioRxiv - Microbiology 2022Quote: ... the remaining lysate was mixed with 20 μl HA beads (Sigma Aldrich) which were prior washed three times with IP lysis buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The lysate was then filtered through a single layer of miracloth (Millipore) into 50ml falcon tubes or 1.5ml Eppendorf tubes on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... Remaining lysate was added to 40 µL of magnetic FLAG beads (Sigma) and incubated overnight at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2019Quote: ... the lysate was incubated with specific antibodies against CTCF (07-729; Millipore), RAD21 (ab992 ...
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bioRxiv - Cell Biology 2020Quote: Whole cell lysates were prepared with RIPA buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were incubated with anti-FLAG M2 affinity gel (A2220, Sigma) overnight and immunoprecipitated ARRB1-FLAG were eluted with Flag peptides (F3290 ...
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bioRxiv - Physiology 2021Quote: ... muscle whole-cell protein lysates were diluted in MPER buffer (Sigma-Aldrich) supplemented with protease and phosphatase inhibitor cocktails ...
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bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The resulting lysates were then used in the aconitase assay (Sigma-Aldrich) in a 96 well plate format according to the kit instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were obtained via mechanical breakage with acid-treated glass beads (Sigma), using a BeadBeater homogenizer for 10 repetitions at 4.5V of 30s on ...
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bioRxiv - Molecular Biology 2019Quote: ... Tissue lysates were mixed with 1-bromo-3-chloropropane (B9673, Sigma-Aldrich) at a ratio of 1:4 and the RNA containing aqueous/organic phase was separated using 5Prime PhaseLock Gel Heavy (#2302830 ...
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bioRxiv - Cell Biology 2020Quote: ... Precleared lysates were immunoprecipitated with M2 anti-flag agarose resin (Sigma, A2220) or G1 Anti-DYKDDDDK affinity resin (GenScript ...
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bioRxiv - Genetics 2020Quote: ... The remaining lysate was incubated with anti-HA-agarose beads (Sigma, A2095) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 10 µl of HPC4 coupled beads (Sigma) for 3 h at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The obtained crude lysate was treated with 50 U benzonase (Sigma, E1014) per 1 mL of lysate at 37°C for 1.5 h to degrade genomic DNA ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated with 20 μl ANTI-FLAG M2 affinity gel (Sigma) for 3 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Lysate was incubated with 10 μg anti-FLAG mouse IgG1 (M2, Sigma) at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and freshly prepared 1M DTT (dithiothreitol, 1uL/5uL cell lysate, Sigma D9779) was added ...
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bioRxiv - Genomics 2021Quote: ... Cell lysate was incubated with anti-FLAG M2 Magnetic Beads (Sigma, #M8823) at 4 °C for 4 h ...
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bioRxiv - Neuroscience 2022Quote: ... we incubated neuron lysates with radioimmunoprecipitation assay lysis buffer (RIPA, Sigma-Aldrich) containing a complete EDTA-free protease inhibitor cocktail (Roche ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was applied to a 10 ml Ni-NTA (Sigma-Aldrich) affinity column ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were transferred to Immobilon-P PVDF Membrane (Sigma-Aldrich Ref. IPVH00010) at room temperature in transfer buffer (25 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysates were applied to a heparin-agarose type I beads (Sigma) previously equilibrated with 0.2% NP-40 in phosphate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates were incubated with anti-FLAG M2 Affinity Gel (SIGMA, A2220) overnight with tumbling at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were then passed through a 0.22 μm Syringe filter (Millipore) and stored at 4 °C.
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bioRxiv - Physiology 2022Quote: ... Lysates were incubated with anti-phospho-Tyr-4G10 (EMD Millipore 05-321), anti-p110α (CST #4249 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated for 30 min with Benzonase (cat# E1014-5KU, Sigma) and cleared by centrifugation for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... protein lysates were separated and transferred onto polyvinylidene fluoride membranes (Millipore, USA). After being blocked with five-percent non-fat dry milk in Tris-buffered saline Tween-20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were treated with 3 μl of Benzonase (25 U/µl, Sigma) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and the clarified lysate was mixed with anti-FLAG M2 resin (Sigma) for 2 h while rotating at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were incubated with 20 μl protein G-coated Sepharose (Sigma-Aldrich) previously conjugated with 5 μl of anti-FLAG antibody by overnight incubation ...
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bioRxiv - Cell Biology 2023Quote: ... the lysate was incubated with ANTI-Flag M2 Affinity Gel (Sigma, USA) or Pierce anti-HA agarose (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 25μg protein lysate were resuspended in Laemmli buffer 1X (Sigma, S3401-1VL), denatured at 95°C for 5 minutes and loaded onto a polyacrylamide gel (Mini-PROTEAN(R ...
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bioRxiv - Immunology 2023Quote: ... pre-cleared lysate were immunoprecipitated with 5 μg ant-IgG (Sigma-Aldrich) or 5 μg anti-Foxp3 (eBioscience ...
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bioRxiv - Biochemistry 2023Quote: ... The bacterial cell lysates were then incubated with glutathione agarose (Sigma–Aldrich) overnight at 4 ºC with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... Cell lysates were incubated with Protein G sepharose beads (Sigma-Aldrich P3296) with anti-RpoC antibody (Biolegend clone NT73 ...
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bioRxiv - Immunology 2023Quote: ... Lysates were acid treated with 50 mM HCl (Sigma-Aldrich #320331-300ML) and then boiled at 95°C for 7 min ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were mixed with anti-FLAG M2 magnetic beads (Sigma-Aldrich) and rotated at 4°C overnight ...
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bioRxiv - Physiology 2023Quote: ... Lysates were digested with 60 ng of sequencing grade trypsin (Sigma; #T6567) and 60 ng of sequencing grade LysC (Wako ...
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bioRxiv - Biochemistry 2023Quote: ... Combined lysates were then incubated with anti-HA agarose beads (Sigma, A2095) for 4 hours at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell lysates were prepared with RIPA buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was filtered through 2-layers of miracloth (Millipore, Cat. 475855) set up in a 50[ml centrifuge tube ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lysate was then acidified to 1 % trifluoroacetic acid (Sigma Cat. No. 302031) for DNA hydrolysis ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates were obtained with SDS supplemented with Laemmli Buffer 2x (Sigma). Samples were boiled for 10 min at 94°C before loading on polyacrylamide gels (4-15% Bis-Tris Gel ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were pre-cleared with 15 µL sepharose-4B beads (Millipore Sigma) for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: Whole tissue or cell lysates were produced in RIPA buffer (Sigma-Aldrich) supplemented with PhosSTOP- and Protease inhibitor cocktail (Roche Applied Science) ...
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bioRxiv - Cell Biology 2023Quote: ... the lysate was mixed with anti-FLAG M2 affinity gel (Sigma-Aldrich) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with anti-Flag (M2 monoclonal antibody, Sigma-Aldrich, #F1804) bound protein A/G magnetic beads (Thermo-Fisher ...