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Citations for Millipore Sigma :
1 - 50 of 2885 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... stained for VZV glycoprotein E (gE) (MAB8612, Millipore) in BD PermWash ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Neuroscience 2023Quote: ... His•Tag® (Millipore, 70796-3), anti-APP C-Terminal Fragment (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... LPS (E. coli O111:B4, Sigma) was dissolved in PBS to make a stock solution then sonicated in a water bath for 10 min prior to addition to cell suspensions ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Plant Biology 2020Quote: ... coli using a Nickel-His column (Sigma). A 5 mL culture was incubated at 37°C for 16 hours ...
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bioRxiv - Cancer Biology 2021Quote: LPS derived from Escherichia coli (E. coli) was from Sigma-Aldrich Canada (Oakville ...
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bioRxiv - Microbiology 2023Quote: ... coli strain BL21 (DE3) (E. coli BL21, EMD Millipore, Cat#69450). F ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Microbiology 2022Quote: ... Mouse monoclonal antibody MAB8150 against WNV E glycoprotein was from EMD Millipore (Billenca MA). Alexa Fluor 488 goat anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2019Quote: E coli Rosetta (DE3) pLysS cells (Novagen) transformed with pOPIN-B plasmids encoding wtGATA3 and neoGATA3 were grown in autoinduction media supplemented with 50 µg/ml kanamycin and 34 µg/ml chloramphenicol for 8 h at 37 °C followed by 21 h at 20 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Biochemistry 2023Quote: Escherichia coli (E. coli) Rosetta2(DE3)pLysS cells (Novagen, cat. no. 71403) were transformed with pET28a-MDA5ΔL2-His6 and grown in 2xTY medium to OD600 0.4-0.6 at 37°C ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells expressing coronavirus spikes were generated by transient transfection using pcDNA3 or pCMV3-based expression plasmids and GeneJuice (EMD Millipore) 48 hours prior to use ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biochemistry 2022Quote: ... 50ng/mL LPS (E. coli O111:B4, Sigma) and 10ng/mL IFN-γ (R&D Systems ...
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bioRxiv - Microbiology 2019Quote: ... ampicillin (Sigma; 100 μg/mL for E. coli), gentamicin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: LPS (Sigma Aldrich, E. coli, serotype 0111:B4), was prepared by dissolving 0.3 mg of LPS in 1ml of PBS (Phosphate buffer saline) ...
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bioRxiv - Bioengineering 2022Quote: ... and LPS (lipopolysaccharides from E. coli; Sigma L8274); HEK-293Ta showed the strongest response to TNFα and were thus selected as responder cells in the co-culture assay (Fig ...
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bioRxiv - Microbiology 2022Quote: ... or tRNA (from E. coli MRE 600, Sigma) was incubated with PT mutants ...
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bioRxiv - Microbiology 2022Quote: ... anti-coronavirus antibody (MAB9013, Merck Millipore), SARS-CoV-2 nucleocapsid antibody (HL344 ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: Purified SARS-CoV-2 spike glycoproteins were exchanged to water using Microcon Ultracel PL-10 centrifugal filter (Millipore Sigma). Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and expressed in BL21 Rosetta 2 (DE3) pLysS Escherichia coli (E. coli) cells (Novagen). All plasmids were verified by Sanger sequencing (OSU Shared Resources) ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 labelled bacteria (E. coli, Sigma-Aldrich) and yeast (S ...
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bioRxiv - Immunology 2021Quote: ... LPS (E. coli serotype O55:B5, Sigma, 10ng/ml), CpG ODN 1826 (TIB MOLBIOL ...
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bioRxiv - Neuroscience 2023Quote: LPS (E. coli, O111:B4 serotype, Sigma Aldrich, UK) was dissolved at 0.5 mg/ml in sterile 0.9% saline ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Genomics 2022Quote: ... These 16 spike-in oligomers were purchased in two separate batches (Table S1) from Sigma Aldrich and resuspended in 10 mM Tris (pH 8 ...
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bioRxiv - Microbiology 2020Quote: Overexpression of was performed by using Escherichia coli (E. coli) Rosetta (DE3) pLysS cells (Novagen) harboring the E ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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Neuraminidase inhibitors rewire neutrophil function in vivo in murine sepsis and ex vivo in COVID-19bioRxiv - Immunology 2022Quote: ... LPS (1 µg/mL, E. coli 0127:b8, Sigma-Aldrich), LPS plus Oseltamivir or LPS plus Zanamivir ...