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Citations for Millipore Sigma :
251 - 300 of 2885 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... strep tag and myc tag were obtained from Sigma, IBA and Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Coronavirus OC43 (ATCC VR-1558) was propagated in HCT-8 cells and detected by antibody staining (MAB9012, Millipore).
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bioRxiv - Synthetic Biology 2021Quote: ... rhaeticus cultures were grown shaking to mid exponential phase. Cells treated with 50 nM (E. coli) or 50 μM (K. rhaeticus) of AHL (N-Acyl homoserine lactone, Sigma-Aldridge K3255) and induced for approximately 4 doubling times (i.e ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μl 1x PBS and 2 μl (1 U) PNGase F (from Elizabethkingia meningoseptica, expressed in E. coli, Sigma Aldrich F8435-50UN) were added to 40 μl of SEC purified YFP-S-RBD (0.24 mg/ml protein concentration) ...
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Microbiology 2020Quote: ... Anti-ICP4 MAb H1A021 or monoclonal Ab to VSV glycoprotein G (P5D4, Sigma) was added for 1 h followed by Alexa Fluor 488-labeled goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Then 1 µg/well of biotinylated α1-acid glycoprotein (Sigma, Catalogue number-112150) or 3’-sialyllactose-PAA-biotinylated (Glycotech ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μM of mRNA oligomer (MF, aMF, or random 24-mer; Sigma Aldrich Corporation), and 250 nM of Sa-RelQ/Sa-RelP/EF-RelQ protein (calculated as a tetramer) ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Plant Biology 2022Quote: ... Various recombinant TFIIIA-7ZF proteins with an intein-chitin binding domain (CBD) tag were overexpressed using the Escherichia coli BL21(DE3) Rosetta strain (EMD Millipore, Burlington, MA). For each construct ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-tag (Sigma), Myc-tag (Upstate) ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
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bioRxiv - Cancer Biology 2022Quote: ... Activation of primary B-cells was induced by the addition of 10 μg/ml LPS (from E. coli, O111:B4, Sigma-Aldrich, Cat#L4391). Ramos cells were authenticated by Eurofins Genomics (Ebersberg ...
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bioRxiv - Neuroscience 2024Quote: ... pups PND 8-9 were given an intraperitoneal (i.p.) injection of lipopolysaccharide (LPS, 40 μg/kg; E. coli O111:B4, Sigma; dissolved in sterile saline) or vehicle (saline) ...
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Molecular Biology 2020Quote: ... coli (Millipore) expressing human LC3B-WT ...
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bioRxiv - Molecular Biology 2021Quote: ... coli (Novagen) for expression of recombinant protein or ΔabmR Mtb for transcriptional activity assays.
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bioRxiv - Bioengineering 2021Quote: ... coli (Novagen) and selected on LB ampicillin with kanamycin B (15 μg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... coli (Novagen). Bacterial cultures were grown to an OD600 of 0.8 at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... coli (Sigma) at 20 ng/mL for NF-κB induction were used as positive controls.
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bioRxiv - Neuroscience 2021Quote: ... coli (Sigma). A batch of mice was used to run a comparative analysis of cortical gliosis in SW ...
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bioRxiv - Physiology 2022Quote: ... coli (Sigma L5293 ...
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bioRxiv - Immunology 2022Quote: ... coli (Novagen) to make high copy plasmids ...
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bioRxiv - Biophysics 2019Quote: ... coli (Novagen). Bacterial cultures were grown to an OD600 of 0.8 at 37°C and then expression was induced with a final concentration of 1 mM of isopropyl β-D-1-thiogalactopyranoside and the growth temperature was reduced to 16°C for 14-16 hours ...
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bioRxiv - Plant Biology 2020Quote: ... coli (Millipore), cultured in LB and induced to express proteins by adding 1 mM IPTG ...
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bioRxiv - Biophysics 2020Quote: ... coli (Novagen) was predominantly used ...
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bioRxiv - Systems Biology 2019Quote: ... coli (Novagen) in minimal (M9 ...
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bioRxiv - Plant Biology 2019Quote: ... coli (Novagen). The expression of the recombinant protein (ending with DSDVD in C-ter ...
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bioRxiv - Microbiology 2021Quote: ... coli (Millipore) for protein expression ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... coli (Millipore) were transformed with the expression vectors ...
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bioRxiv - Immunology 2020Quote: ... coli (Novagen). The bacterial cell pellets were resuspended in the lysis buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Novagen) transformed with pET_-7GFP ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Novagen) was performed as described (Geli et al. ...
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bioRxiv - Microbiology 2022Quote: ... coli (Novagen) by heat-shock and plated on LB with the appropriate selection ...
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bioRxiv - Neuroscience 2022Quote: ... coli (Sigma) in 10 mM ammonium acetate buffer pH 5.3 ...
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bioRxiv - Cell Biology 2023Quote: ... coli (Novagen) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... coli (Novagen) was transformed with each expression vector and grown in 50 ml LB broth containing 100 µg/ml ampicillin at 37°C with shaking until A600 reached between 0.6 and 0.8 ...
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bioRxiv - Cell Biology 2023Quote: ... coli (Novagen) grown in Terrific Broth media ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Novagen) by heat shock ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Novagen). 250 mL to 1 L bacteria cultures were grown in LB broth with ampicillin (100 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... coli (NOVAgen.1 L bacteria cultures in LB broth containing ampicillin (100 μg/mL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (Novagen) and purified by affinity and size exclusion chromatography ...
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bioRxiv - Microbiology 2023Quote: ... coli (Novagen) carrying a modified pET28a expressing either EHEC FruK-C-His or EHEC Cra-C-Flag were grown in 150 mL LB supplemented with kanamycin (50 µg mL−1 ...
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bioRxiv - Immunology 2023Quote: ... coli (Novagen). Cells were lysed with an M-110P microfluidizer (Microfluidics ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Sigma) measured via Amplex red uricase activity assay (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... coli (Novagen) and coselected on ampicillin (100 µg /ml ...
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bioRxiv - Immunology 2023Quote: ... E-64d (Sigma), or 0.5% DMSO ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-spike (Cat#ZMS1076, Sigma Aldrich), mouse anti-Strep (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... The MERS-CoV peptide was labeled with sub-stoichiometric FITC (Sigma-Aldrich, St. Louis, Missouri) in a basic HEPES buffer (pH 8.2 ...
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bioRxiv - Biochemistry 2021Quote: ... The 3’ biotinylated TAR and RNA 30-mer (Table 1) were chemically synthesized (Sigma Aldrich). RNA (50 pmol ...