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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... anti-His (Millipore: 05-949, clone HIS.H8) or Goat anti-Mouse IgG (Millipore ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and His-Bind Purification kit (Novagen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... L-histidine (L-his, Sigma-Aldrich #H8125), L-ornithine (L-orn ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Biochemistry 2022Quote: ... residual bound Coa and Efb were detected with anti-tag HRP-conjugated antibodies diluted 1:10000 in 2%BSA-PBST: α-HIS-tag antibody (A7058, Sigma) for EfbN ...
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bioRxiv - Neuroscience 2024Quote: ... The tissues were mashed through a 70-μm cell strainer in PBS / 2% heat-inactivated fetal bovine serum (HI-FBS; Sigma-Aldrich) / 10 mM EDTA-Na (pH 8.0) ...
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bioRxiv - Neuroscience 2019Quote: ... 2 μM Cytosine b-D-arabinofuranoside (Ara-C; Sigma-Aldrich C1768-100MG) was added to remove any proliferating cells ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μM Cytosine β-D-arabinofuranoside (Ara-C; Sigma-Aldrich C1768-100MG) was added to remove any proliferating cells ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μg/ml Vitamin C (L-Ascorbic acid 2-phosphate, Sigma A8950), 1% GlutaMAX (ThermoFisher 35050061 ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μg/ml Vitamin C (L-Ascorbic acid 2-phosphate, Sigma A8950), 20 ng/ml recombinant human LIF (Peprotech 300-05) ...
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bioRxiv - Immunology 2019Quote: ... for 30 minutes at 37°C and fixed in 2% PFA (Sigma) for 15 minutes at room temperature ...
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bioRxiv - Physiology 2023Quote: ... were lysed at 2°C in lysis buffer (0.5% CHAPS (Millipore-Sigma), 150 mM NaCl ...
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bioRxiv - Physiology 2023Quote: ... were lysed at 2°C in lysis buffer (0.5% CHAPS (Millipore-Sigma), 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were incubated for 1.5 hours at 4°C with purified human IgG (Sigma) that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: ... substrates containing 400 nm diameter pores at a density of 2·106 pores·cm-2 were coated with 100 µL of 1 µg/mL human plasma fibronectin (Sigma-Aldrich) in PBS (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Neuroscience 2021Quote: ... These coverslips were left to grow at 37°C and 5% CO2 and were treated with 2 µl Ara-C (Sigma) on day in vitro 1 (DIV1 ...
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bioRxiv - Genomics 2022Quote: ... Cross-links were reversed by incubating the eluted samples and inputs at 42°C for 2 hours and 67°C for 6 hours in the presence of Pronase (Sigma) and TE buffer ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were cultured in DMEM-F12 (cat n. D6421, Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: HEK293 cells plated on covered with Poly-D-Lysine (P0899, Sigma-Aldrich) coverslips were co-transfected with pEZY BIFC N-Venus and C-Venus plasmids 48 hours after plating ...
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bioRxiv - Neuroscience 2022Quote: ... 0.8×105 HEK293 cells were seeded on poly-L-ornithine (Millipore Sigma) pre-coated 12 mm coverslips ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were grown in DMEM 4.5 g/L glucose (D6429, Sigma) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... while HeLa and HEK293 cancer cells were maintained in DMEM (Sigma- Aldrich). Media was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2023Quote: ... Stable HEK293-6E cell lines were maintained in F17 media (Sigma Aldrich) supplemented with Kolliphor p188 (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Physiology 2022Quote: MBP was purified from E.coli as follow: Rosetta (DE3) cells (catalog no. 70954, Sigma-Aldrich) transformed with pOPTM were incubated ON at 37°C in SOB media (2% Tryptone ...
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bioRxiv - Neuroscience 2020Quote: ... chicken anti-P0 1:300 (Aves #PZO0308) and mouse ant-MBP 1:300 (Millipore #MAB384). Images from teased fibers were acquired at 40X magnification and 1.5X zoom using a confocal microscope Leica SP5II running the LAS AF 2.7.9723.3 software (Leica) ...
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bioRxiv - Neuroscience 2019Quote: Antibodies used were: rat anti-myelin basic protein (MBP, 1:200, MAB386, Millipore, MA, USA), rabbit anti-Olig2 (1:200 ...
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bioRxiv - Biochemistry 2022Quote: ... Purified 6xHis-MBP-FUS was buffer-exchanged by concentrating FUS in Amicon filters (Millipore Sigma) and diluting with 20 mM Na2HPO4 pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Rats in the EAE group were immunized with subcutaneous injections of 100 µg MBP (Sigma) emulsified in 5 mg/mL complete Freund’s adjuvant (CFA ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with primary antibodies for rat anti-MBP (1:500; Millipore, clone 12) and chicken anti-NFH (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... OEC-2 was cultured on human fibronectin-coated plates (15 μg/ml; EMD Millipore) in serum-free N2B27 medium (DMEM/F12 [Gibco] and Neurobasal [Gibco] 1:1 ratio ...
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bioRxiv - Immunology 2021Quote: ... supplemented as per RF10 above with the addition of 2 % human AB serum (Sigma).
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bioRxiv - Microbiology 2023Quote: ... 10 μg/mL goat anti-human IgG1 Fc F(ab’)2 (Sigma-Aldrich, I3391) was coated in 96-well NUNC Immunoplate (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Molecular Biology 2023Quote: ... or 2 µg of MATR3 siRNA (MISSION esiRNA human MATR3 Sigma-Aldrich EHU113801-50UG) using Lipofectamine 3000 Transfection Reagent (ThermoFisher Scientific L3000015 ...
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bioRxiv - Cell Biology 2024Quote: ... 2×106 sperm/mL were incubated in human tubular fluid (HTF) medium (EMD Millipore) at 37°C ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Genetics 2019Quote: Small-interfering RNAs (siRNA) targeting human C/EBPβ were purchased from Sigma Aldrich (St Louis, MO, USA). siRNA ID ...
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bioRxiv - Genomics 2023Quote: ... Thawed PBMCs were quenched by adding 12 mL 37°C pre-warmed human serum (Sigma Aldrich, # H4522) and were centrifuged at 500 x g for 5 min at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were thermally aged overnight at 37 °C and then 60 min at 60 °C, followed by treatments with RNase A (200 µg/mL in 2× SSC, 60–90 min, 37 °C) (Sigma-Aldrich) and then pepsin (50 µg/mL in 10 mM HCl ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...