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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... About 3x106 cells were immunoprecipitated using 10 µg of a monoclonal SF3B1 antibody (clone 16, MBL D221-3) or 10 µg of the monoclonal U2AF2 antibody (U4759, SIGMA).
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against some cytoskeletal proteins such as vimentin (1:50 in PBS; o/n at 4°C + 2 hr at 37°C) (Sigma; V6630, USA), pericentrin (1:1000 in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... Fixed plant material was digested at 37 °C in the PINE enzyme mixture consisting of 2 % (w/v) cellulase from Aspergillus niger (Sigma C-1184), 4 % (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes before incubating in c-Fos primary antibody for 2 days (rabbit anti c-Fos, 1:1000; Millipore, Burlington, MA). Sections were then washed in 3x in TBS with 0.3% Triton X-100 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with 10 µg/ml of capture anti-human IgG (Sigma Aldrich, I2136) in PBS ...
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bioRxiv - Biophysics 2021Quote: ... Cardiomyocytes were attached to imaging chambers (laminin-coated coverslips attached to acrylic slides) for 2 hours at 37°C and fixed in 2% paraformaldehyde (Sigma-Aldrich) (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated with frequent agitation at 37°C for 20 min in 2 ml of 2 mg/ml papain (Sigma, P4762) solution in Hibernate-A ...
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bioRxiv - Plant Biology 2022Quote: ... 0.85 % saline (2 mins), Phosphate Buffered Saline (PBS) (2 mins), 0.125 mg ml-1 Pronase (37°C, 10 mins) (Sigma-Aldrich, P2308), 0.2 % Glycine (2 mins ...
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bioRxiv - Immunology 2023Quote: Yp was cultured to stationary phase at 28°C and 250 rpm shaking for 16 hours in 2×YT broth supplemented with 2 μg/ml triclosan (Millipore Sigma). Mice were fasted for 16 hours and subsequently inoculated by oral gavage with 200 μl phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... 50µg/ml Vitamin C (L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate - Sigma A8950), 20 ng/ml recombinant human LIF (Peprotech 300-05 ...
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bioRxiv - Molecular Biology 2021Quote: ... All lentivectors and VLPs were DNAse treated (2 h at 37°C, DNaseI, Sigma) before they were concentrated by ultracentrifugation in a Sorvall Discovery (Hitachi ...
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bioRxiv - Biochemistry 2019Quote: ... 4.5 mM MgCl2) preincubated at 37°C containing 2 μg/mL DAPI (Sigma-Aldrich) in the presence or absence of 5 mM ATP disodium salt (Oriental Yeast ...
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bioRxiv - Developmental Biology 2020Quote: ... 50µg/ml Vitamin C (L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate - Sigma A8950), 20 ng/ml recombinant human LIF (Peprotech 300-05 ...
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bioRxiv - Microbiology 2023Quote: ... or (2) for 1 hour at 37°C using Subtilisin A (P5380, Sigma-Aldrich) with the same concentration as for the three other enzymes ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 1 hour at 4 °C with 2% rat serum (Sigma) to reduce antibody binding to Fc receptors ...
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bioRxiv - Genetics 2024Quote: ... and incubated for 2 hours at 4°C with anti-FLAG M2 antibody (Sigma)-coupled Dynabeads (Invitrogen) ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Molecular Biology 2023Quote: ... was incubated for 2 h at 55 °C and for 10 min at 95 °C in 50 μL lysis solution (proteinase K -Sigma-Aldrich, Munich, Germany) freshly diluted 1:100 in lysis buffer (25 mM NaOH ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were seeded on to #0 coverslips coated with poly-L-lysine (Sigma) in a 24-well culture dish and kept in an incubator at 37°C under air with 5% CO2 ...
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bioRxiv - Biochemistry 2019Quote: HEK293 cells were grown in DMEM (SLRI media facility) plus 10% FBS (Sigma, F1051). For analysis of SMN chemical antagonists ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) in optiMEM solution for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) and submitted to selection with G418 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... Blocking solution for HEK293 and iPS cells respectively contained 10% normal goat serum (Sigma) or fetal bovine serum (Eurobio ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were grown in EX-CELL® ACF CHO Medium (Sigma-Aldrich, C5467) supplemented with 100 IU/ml penicillin and 100 μg/ml streptomycin at 37°C with 95 % humidity and 5 % CO2 ...
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bioRxiv - Cell Biology 2022Quote: HEK293 (ATCC, CRL-1573) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D5796) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma, #F0804) and 1% Penicillin/Streptoymycin (HyClone ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HEK293 cells stably expressing FLAG-GPR65 were plated to poly-d-lysine (Sigma Aldrich) coated 12 mm glass coverslips (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293-TRPM2tet cells were treated with tetracycline hydrochloride (1 µg/ml; T7660, Sigma-Aldrich) for 48 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 48-well cell cultures were supplemented with 100 µM guanosine (Sigma Aldrich #G6264). DMSO and 100 µM adenosine (Sigma Aldrich #A9251 ...
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bioRxiv - Cell Biology 2019Quote: ... Aurora B (0.01 µg/µl) and myelin basic protein (MBP; 2.5 µg) (Sigma-Aldrich and Biaffin GmbH) or one of the three Mid1p domains (“N-term” ...
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bioRxiv - Plant Biology 2022Quote: Protein phosphorylation was performed by incubating 1 µg of PpeAld6PRase or myelin binding protein (MBP; Sigma-Aldrich) with the corresponding protein kinase in a standard medium containing 20 mM Tris-HCl pH 7.2 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GSTπ at 1:3000 (MBL, #311), rabbit anti-IBA1 at 1:500 (Wako, #01919741) and rabbit anti-GFAP at 1:1000 (Millipore, #OBT0030). Sections were washed three times with PBS and stained using biotinylated anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Microbiology 2021Quote: ... human colorectal adenocarcinoma cell line Caco-2 were maintained in Dulbecco’s Modified Eagle’s Media (Sigma-Aldrich) supplemented with 10% FCS (Fetal calf serum ...
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bioRxiv - Molecular Biology 2023Quote: ... sequences flanking the Human TRR-TCT3-2 gene are in italics) was ordered from Sigma Aldrich. Non-template and template strands were annealed in H2O followed by heating to 95 ⁰C for 5 min before cooling down to 20 ⁰C at a rate of 1 ⁰C/min ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were washed with 60x slurry volume in lysis buffer and eluted through overnight incubation at 4°C with 160μl of Human Rhinovirus (HRV)-3C protease (Millipore) in a final volume of 2-3ml ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Cancer Biology 2022Quote: ... human tumor slides were stained by immunohistochemistry with an antibody against Tenascin-C (1:300, EMD Millipore, Cat. ab19011), a strain-sensitive ECM protein known to be present in LUAD [9 ...