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Citations for Millipore Sigma :
501 - 550 of 4288 citations for IL 9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... plates were developed using 3-amino-9-ethyl-carbazole (AEC) substrate (Sigma). The developed plates were scanned and spots were analyzed using an automated ELISpot counter (Cellular Technologies Ltd.).
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bioRxiv - Cell Biology 2022Quote: ... DNA was complexed with X-tremeGENE 9 DNA Transfection Reagent (Sigma, 6365779001) in OptiMEM according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 0.5 μg/mL doxycycline (Sigma; 10592-13-9).
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bioRxiv - Microbiology 2022Quote: ... 25 pmol cholesteryl ester (CE, 9:0, 19:0, 24:1, Sigma), and 24 pmol triacylglycerol (TAG ...
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bioRxiv - Biophysics 2022Quote: ... and 2 µM 9-Amino-6-Chloro-2-Methoxyacridine (ACMA, Sigma-Aldrich) to establish a K+ gradient ...
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bioRxiv - Immunology 2023Quote: ... Slides were incubated in eriochrome cyanine R (Sigma-Aldrich, #3564-18-9) staining solution (0.22% w/v ferric chloride ...
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bioRxiv - Cell Biology 2023Quote: ... and 230 µl of the 9 mol/L sulphuric acid (Merck Millipore) was added and mixed ...
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bioRxiv - Bioengineering 2023Quote: ... adjusting the pH to 8–9 with sodium hydroxide (Sigma-Aldrich, 71687). The solutions for the labeling pipeline were pumped inside the animal’s body by transcardial-circulatory perfusion ...
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bioRxiv - Microbiology 2023Quote: ... O-phthalaldehyde (OPA) and 9-fluorenylmethyl chloroformate (FMOC) were purchased from Sigma Company ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... After staining the cells with crystal violet (548-62-9, Sigma-Aldrich), colony sizes were measured using ImageJ 1.53t (Bethesda ...
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bioRxiv - Developmental Biology 2023Quote: ... or vehicle alone (dimethyl sulfoxide [D8418, Sigma-Aldrich]/corn oil, 9:1) 30 minutes before euthanization.54 To examine the relationship between IGF-1 and PI3K signaling under nutritional deprivation ...
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bioRxiv - Cell Biology 2023Quote: MCF-10A cells were treated with 9 μM RO-3306 (Sigma #SML0569), dissolved in DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... binding was visualized using 3-amino-9-ethylcarbazole (AEC) (Sigma-Aldrich, Germany) and slides were counterstained using hematoxylin.
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bioRxiv - Evolutionary Biology 2023Quote: ... vancomycin (10 µg / mL) (Sigma Aldrich, V2002, CAS number 1404-93-9), nystatin (1 U / mL ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293F cells were stably transfected (XtremeGENETM 9 DNA transfection reagent; Sigma-Aldrich) according to the manufacturer’s protocol with pcDNA3.1 + encoding human dystroglycan residues 1–749 with a mutation in the furin cleavage site (R311A/R312A) ...
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bioRxiv - Neuroscience 2024Quote: ... 9 days before euthanasia and 15 mg/kg of Alizarin (Sigma-Aldrich) 2 days before euthanasia ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 0.5 μg/ml doxycycline (Sigma; 10592-13-9).
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Microbiology 2022Quote: For immunofluorescence experiments the panel of HEK293 cells grown on poly-Lysine (Sigma-Aldrich Cat. Nr. P6282) pre-coated (2.5 μg/mL in MQ H2O 30 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...