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Citations for Millipore Sigma :
451 - 500 of 4288 citations for IL 9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... IL-4 (20 ng/mL, Sigma, 11276905001) or PBS ...
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bioRxiv - Biophysics 2019Quote: ... and 50 U/ml IL-2 (Sigma) followed by spin-infection for 90 minutes at 30°C and 1000g ...
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bioRxiv - Immunology 2022Quote: ... and 10U/mL IL-2 (11147528001, Sigma).
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bioRxiv - Neuroscience 2023Quote: ... IL-1α (3 ng/mL, Sigma I3901) and TNF (30 ng/mL ...
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bioRxiv - Genetics 2023Quote: ... and IL-6 (#RAB0309, Sigma Aldrich, GmbH) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... 5 ng/ml IL-4 (Sigma I1020), and 20 µg/ml LPS (Sigma L-2630) ...
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bioRxiv - Biophysics 2024Quote: IL-2 (Sigma Aldrich Cat #: I0523-5X20UG) was added to a final concentration of 1.25 µg/mL on Day 2 ...
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bioRxiv - Immunology 2024Quote: ... and IL-13 10ng/ml (Sigma-Aldrich) were isolated ...
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bioRxiv - Immunology 2020Quote: Cell lines (HEK293T, HEK293, 3C11, 143B, MDCK-SIAT1, MDCK-PR8) were maintained in DMEM (Sigma Aldrich) supplemented with 10% FCS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
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bioRxiv - Bioengineering 2021Quote: ... for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG; Sigma Aldrich) before seeding cells onto the 8-well plates ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were seeded in 8-well Nunc Lab-Tek II chamber slides (0,7 cm2, Sigma) at a density of 70.000 cells/cm2 ...
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bioRxiv - Immunology 2020Quote: ... Protein production was done in HEK293-6E cells by transient transfection with polyethylenimine (PEI, Sigma-Aldrich) and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: 2×105 HEK293 were seeded onto 24well plates previously pre-coated with poly-L-lysine (Sigma), and infected with 2×105 ...
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bioRxiv - Immunology 2020Quote: ... this mixture was applied to mammalian FreeStyle™ HEK293-F cell culture (EMD-Millipore, Darmstadt, Germany) at a density of 1 million cells per milliliter ...
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bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
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bioRxiv - Immunology 2021Quote: ... it was added the substrate 3-Amino-9-ethylcarbazole (AEC) (Sigma-Aldrich), for 20 minutes ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... and developed with chromogen substrate: 0.03% 3-amino-9-ethylcarbazole (Sigma-Aldrich) and 0.015% H2O2 in 1M sodium acetate ...
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bioRxiv - Molecular Biology 2020Quote: ... binding was visualized using 3-amino-9-ethylcarbazole (AEC) (Sigma-Aldrich, Germany) and slides were counterstained using hematoxylin.
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bioRxiv - Immunology 2020Quote: ... and 3’-amino-9-ethyl carbazole (AEC Tablets, Sigma, St. Louis, MO) were further used to develop the plate ...
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bioRxiv - Developmental Biology 2020Quote: ... mice were administered 9 mg per 40 g body weight tamoxifen (Sigma) suspended in corn oil by oral gavage once daily for 4 consecutive days ...
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bioRxiv - Neuroscience 2020Quote: ... the pan-glutamate receptor antagonist kynurenic acid (Sigma, 40mM, 8.5-9 pH) was filled in one barrel of the glass electrode and microointophoretically applied to the recording site ...
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bioRxiv - Bioengineering 2021Quote: ... the tissues were treated with buffered eosin (1:9 = eosin (HT110216, Sigma): 0.45M Tris-Acetic buffer (pH 6.0)) ...
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The anti-lipidemic drug simvastatin modulates Epigenetic Biomarkers in the Amphipod Gammarus locustabioRxiv - Pharmacology and Toxicology 2020Quote: ... Simvastatin (CAS number 79902-63-9, ≥ 97%) was purchased from Sigma-Aldrich and was prepared in acetone ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were treated with CDK1i (9 μM for 16h; Sigma-Aldrich, SML0569) or decitabine (DAC ...
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bioRxiv - Neuroscience 2020Quote: ... Cav3.1 (1:500; Cat. no. MABN464, Sigma-Aldrich, NeuroMab clone N178A/9), β-galactosidase (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and the transfer vector using Xtreme gene 9 transfection reagent (Sigma-Aldrich). Two days after transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized in PBS/PVP containing 0.25% Triton X-100 (23,472-9, Sigma) for 30 minutes and blocked in buffer comprising PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 6-9 COCS were plated per PTFE-coated cell culture insert (Millipore) in organotypic slice culture medium [134] ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.2mg/ml bovine pancreas DNase (Sigma part no. 9003-98-9) were added ...
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bioRxiv - Microbiology 2020Quote: ... and HDAC inhibitors Suberoylanilide hydroxamic acid (SAHA, 149647-78-9, Sigma Aldrich), TMP-195 (23242 ...
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bioRxiv - Immunology 2021Quote: ... plates were developed using 3-amino-9-ethyl-carbazole (AEC) substrate (Sigma). The developed plates were scanned ...