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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for IGFBP 7 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... embedded into 7% agarose (Sigma Aldrich A0701), and sliced using a vibratome (VT 1000S ...
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bioRxiv - Immunology 2024Quote: ... embedded into 7% agarose (Sigma Aldrich A0701) and then cut into 150µm-thick sections through vibratome (VT 1000S ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Biochemistry 2020Quote: ... 7-amido-4-methyl-coumarin (NHMec) from the commercially available substrate Leucine-7-amido-4-methyl-coumarin (Sigma-Aldrich). For processivity assays ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... taurine (CAS 107-35-7) and ascorbic acid (CAS 50-81-7) were obtained from Sigma-Aldrich (Taufkirchen, Germany). 1M sucrose solutions (Südzucker AG ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed with TBST buffer (three times, 7 minutes each) and incubated with anti-mouse-HRP or anti-rabbit-HRP (Sigma-Aldrich; 20,000 times dilution) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... Component DNA plasmids were transfected into Lenti-X-HEK-293T cells via GeneJuice® (Novagen) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T cells were seeded onto acid-washed coverslips coated with 0.1% gelatin (Sigma-Aldrich) in 24-well plates at 5×104 cells/well ...
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bioRxiv - Cancer Biology 2022Quote: ... Human embryonic kidney HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma). Cell culture media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were co-transfected intro HEK 293T cells via X-tremeGene 9 reagent (Millipore Sigma #6365787001). Two days later ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK-293T cells were grown in Dulbecco’s modified Eagle’s medium (Sigma-Millipore, Cat No. D5796). Both types of media were supplemented with penicillin and streptomycin (Corning ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK-293T cells were grown in Dulbecco’s modified Eagle’s medium (Sigma-Millipore, Cat No. D5796). Both types of media were supplemented with penicillin and streptomycin (Corning ...
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bioRxiv - Bioengineering 2019Quote: Genotoxicity assay was determined using Hek 293T cells (Human embryonal kidney cells, Sigma-Aldrich, USA) according to Comet assay as previously described [31] ...
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bioRxiv - Microbiology 2022Quote: Lysates from transfected HEK 293T cells were lysed in 1x RIPA + protease inhibitor cocktail (Sigma). Lysates were clarified by centrifugation at 4 °C and 12,000 xg for 10 min ...
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bioRxiv - Synthetic Biology 2023Quote: HEK-293T (ATCC; CRL-11268) cells were cultured in Iscove’s Modified Dulbecco’s Medium (Sigma; I3390) supplemented with 10% FBS (Biosera ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HEK-Slack cells were plated onto poly-D-lysine-coated (100 μg/mL, Sigma Aldrich) coverslips 1 d before experiments and cultured in DMEM containing 10% FBS and 1% penicillin/streptomycin in 5% CO2 at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: HEK-293T cells were transfected with indicated plasmids using GeneJuice (70967, Novagen, San Diego, CA) and Opti-MEM (51985 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed and resuspended in PBS 4%BSA with viability staining reagent 1μg/ml (7-AAD) 7-aminoactinomycin D (Sigma Aldrich). Stained cells were analyzed with a MACSQuant 10 (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2022Quote: ... for 7 days followed by 7 days treatment with phosphate buffered saline (PBS) and 0.2% dimethyl sulfoxide (DMSO, Sigma-Aldrich). Lastly ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed and resuspended in PBS 4%BSA with viability staining reagent 1μg/ml (7-AAD) 7-aminoactinomycin D (Sigma Aldrich). Stained cells were analyzed with a MACSQuant 10 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...