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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for IGFBP 7 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: The custom designed CompoZr® Zinc Finger Nuclease Plasmid targeted to exon 3 of the mouse ECH1 gene on chromosome 7 was obtained from Sigma. In-vitro transcription of the ZFN mRNA was performed following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Immunology 2019Quote: ... HEK-293T cells were plated on poly-L-Lysine-coated (Sigma; P4707) 10 cm plates (Corning ...
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bioRxiv - Cell Biology 2022Quote: BEAS-2B and HEK-293T cells (ATCC) were grown in DMEM (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: HEK 293T cells were cultured in DMEM with 10% FBS (Millipore Sigma), GlutaMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... HCC5044 and HEK-293T cells were cultured in DMEM (Sigma-Aldrich, D5796) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: ... TPC-1 and HEK 293T/17 were cultured in DMEM (Sigma D6429) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 7-deaza-2’-deoxy-guanosine-5’-triphosphate (7-Deaza-dGTP)(10988537001, Sigma-Aldrich) to aid in amplifying GC rich regions ...
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bioRxiv - Neuroscience 2020Quote: ... 7-Deacetyl-7-[O-(N-methylpiperazino)-gamma-butyryl]-dihydrochloride (20µM; 344273, EMD Millipore, Billerica, MA) was dissolved in sterile Milli-Q water ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Bioengineering 2024Quote: ... typically in an equal mixture of Expi293 and EX-CELL 293 (14571C Sigma). Expi293 media can be used for the entire process ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Cancer Biology 2021Quote: ... The exclusion of dead cells was done by adding 7-aminoactinomycin D (7-AAD) (Sigma-Aldrich) to the cell suspension to the final concentration of 1 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... 7 ng/mL glucagon (Sigma-Aldrich), 0.1 mM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... 7 μM Hemin chloride (Sigma-Aldrich) was added to the culture medium and kept in the medium until the fixation step (below) ...
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bioRxiv - Cell Biology 2019Quote: ... 7 µl 2-mercaptoethanol (07604, Sigma) and 690 µl Buffer B (50 mM Tris ...
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bioRxiv - Genetics 2019Quote: ... 7 mM caffeine (C0750, Sigma-Aldrich), 100 mM hydroxyurea (H8627 ...
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bioRxiv - Neuroscience 2019Quote: ... GLUR6/7 (04-921, EMD Millipore), EEA1 (sc-6415 ...
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bioRxiv - Genomics 2019Quote: ... and 1.0M pH 7 from Sigma. Triethylamine acetate buffer 2.0M pH 7 and Sodium hydroxide 1.0M bioreagent were purchased from Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... and 10−7 M insulin (Sigma). The number of viable cells was determined using trypan blue (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... NBQX (2 μg, Sigma, n=7), Bicuculline methiodide (0.05 μg ...
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bioRxiv - Biochemistry 2021Quote: ... mAb-HA (Clone 7, H9658, Sigma), pAb-HA (Signalway Eurogentec) ...
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bioRxiv - Biophysics 2020Quote: ... and ML-7 (I2764, Sigma-Aldrich) were used at 5 μM working concentration ...
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bioRxiv - Bioengineering 2020Quote: ... Glucose (50-99-7, Sigma-Aldrich), DMEM High glucose (4.5g/L ...
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bioRxiv - Plant Biology 2021Quote: 2’,7’ dichlorofluorescein diacetate (H2DCFDA, Sigma) was used to measure ROS in guard cells (49) ...
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bioRxiv - Biochemistry 2021Quote: ... and 7 M urea (Sigma Aldrich)) in 1 x TBE (Tris-borate EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... and 7 M urea (Sigma Aldrich)) in 1 x TBE (Tris-borate EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... and 7 M urea (Sigma Aldrich)) in 1 x TBE (Tris-borate EDTA ...