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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Human Single stranded DNA binding protein 4 SSBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... anti-MAR binding reagent (Millipore, MABE1076), anti-GST (clone 6G9) ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing medium was collected 46 and 65 hours post transfection and was filtered through 0.45 μM Millipore low-protein binding filter units (Millipore SCHVU02RE). Viral supernatant was concentrated by first using low speed centrifugation through 100 kDa MW cutoff Millipore Centricon 70 Plus cartridges to condense volume to < 4mL ...
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bioRxiv - Microbiology 2020Quote: ... Loaded proteins were washed with 300 μL Binding Buffer (90% methanol and 10% triethylammonium bicarbonate buffer (TEAB, Sigma-Aldrich, #T7408), adjusted to pH 7.1 using phosphoric acid ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... Elution of proteins was done by adding 100 µl of binding buffer supplemented with 20 mM IP6-sodium salt (Sigma) and then incubating overnight at 4°C under constant mixing ...
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bioRxiv - Developmental Biology 2021Quote: ... As control for iodide uptake and protein-binding measurements cells were also treated with 30µM sodium perchlorate (Nis inhibitor; NaClO4, Sigma-Aldrich)) and 2 µM methimazole (TPO inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation at 500 g for 10 minutes and filtration through nylon low protein-binding filters (SLHP033RS, Millipore). Virus was concentrated by ultracentrifugation (employing a SW32 rotor at 25K for 2hr and 30min at 4° C ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Cancer Biology 2022Quote: ... Hydrogels were made permissive for protein binding by incubating them in 500 µl of Sulfo-SANPAH solution [0.2 mg/ml Sulfo-SANPAH (Sigma, 803332) and 2 mg/ml N-(3-dimethylaminopropyl)-N′- ethylcarbodiimide hydrochloride (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... 1mL of the supernatant was filtered through a syringe with an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland). The KTI in solution was separated on an Agilent 1260 Infinity series (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed again with 800 μl binding buffer three times and the bound proteins were eluted with the binding buffer supplemented with 10 mM D-desthiobiotin (Sigma) and analyzed with SDS PAGE.
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Molecular Biology 2023Quote: ... the virus-containing supernatant was harvested by gentle pipetting and filtered with a low protein binding syringe filter (0.22 μM, EDM Millipore # SLGV033RB). The virus was frozen at -80 °C for long-term storage or transduced directly into the desired cells.
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bioRxiv - Immunology 2023Quote: ... Protein was harvested from cell culture supernatants and purified by binding to a nickel-nitrilotriacetic acid (Ni-NTA) column (Novagen) followed by elution with 1M imidazole containing 0.2% octyl β-D-glucopyranoside ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Genomics 2024Quote: ... 1 mL of the supernatant was filtered through a syringe using an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic Millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland).
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bioRxiv - Biophysics 2024Quote: ... and the supernatant containing the solubilized protein was collected for affinity purification by batch-binding to 10 mL of M2-agarose FLAG resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... We confirmed the specificity of our IgA ELISA and non-cross reactivity toward IgG using purified human IgG and IgA (Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... cell lysate was incubated with RIP binding buffer containing magnetic beads coated with human Ago2 antibody or mouse IgG antibody (control) (Millipore, Billerica, MA, USA). Subsequently ...
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bioRxiv - Immunology 2019Quote: ... scFv secretion levels were analyzed by ELISA after coating with HEL antigen (Sigma-Aldrich, 62971, 4 μg ml-1) in PBS (Thermo ...
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bioRxiv - Bioengineering 2021Quote: ... cell supernatant was harvested by centrifugation (4000xg for 20 mins at 4°C) and filtered through a low binding 0.22 μm stericup vacuum filter (Millipore Sigma, S2GPU10RE). The filtered supernatant was incubated for 2 hours at room temperature with 6ml of Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... and 500 μg of Flag-GST-T6B peptide with high-affinity binding to Ago1-4 (78) was added coupled with 80 μl of anti-Flag M2 Magnetic beads (Sigma #M8823) and incubated for 3 hrs on a rotor at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The rANF single strands DNAs (5’-AGAGGTCATGAAGGACATT-3’ and 5’AATGTCCTTCATGACCTCT-3’) were purchased from Sigma Aldrich and annealed ...
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Highly Versatile, Non-Invasive Method for Collecting Buccal DNA from Free-Ranging Non-Human PrimatesbioRxiv - Genetics 2021Quote: ... a series of human placental DNA (Sigma-Aldrich, St. Louis, MO, USA) at concentrations of 500 ...
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bioRxiv - Physiology 2021Quote: ... Insulin was measured by ELISA (EZRMI-13K Sigma-Aldrich - Rat/Mouse - Insulin ELISA) and the hepatic and skeletal muscle glycogen contents of fed rats (Lo et al ...
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bioRxiv - Microbiology 2023Quote: ... before staining with a 0.1 % (w/v) solution of the DNA-binding dye 4,6-diamidino-2-phenylindole (DAPI) (Sigma, Deisenhofen, Germany) at room temperature for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Single colonies were inoculated and protein expression was induced using isopropyl-β-D-thiogalacto-pyranoside (IPTG, Sigma Aldrich). Cell lysates were screened for protein expression using SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Systems Biology 2023Quote: Well plates containing single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was purified using GenElute Single Cell RNA Purification Kit (Sigma-Aldrich RNB300), treated with DNase I on column (Sigma-DNASE70-1SET) ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Immunology 2023Quote: ... OVOL1 protein expression were determined by rabbit anti-human OVOL1 antibody (Sigma Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... or Protein G Sepharose beads (Protein G Sepharose® 4 Fast Flow, Sigma-Aldrich, GE17-0618-01) used for anti-Ollas antibody IPs ...
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bioRxiv - Cell Biology 2019Quote: ... eight-week-old mice were injected with a single pulse of tamoxifen (4 mg/25 g body weight, Sigma). To label the Msi1+ cells at homeostasis ...
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bioRxiv - Cell Biology 2024Quote: ... Inducible lineage tracing in the PdgfrαCreERTR26mTmGmodel was performed in adult female mice following a single intraperitoneal injection of 1.25 mg of 4-hyroxytamoxifen (TAM; Sigma) diluted in sunflower seed oil per 25 g body weight to induce Cre-mediated recombination and GFP labeling ...
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bioRxiv - Cell Biology 2020Quote: ... BCA protein Assay Kit (Sigma-Aldrich) was used to quantify proteins in all the fractions and 30 µg of proteins per fraction were separated by SDS-PAGE for immunoblot.
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was performed with the BCA Protein Assay Kit (Millipore); for each sample ...
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bioRxiv - Microbiology 2019Quote: ... The Human Cytokine/Chemokine Magnetic Bead Panel kit (Millipore) was used according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... Human 80 Plex kits were purchased from EMD Millipore Corporation ...
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Neuroscience 2020Quote: ... The filtrate was transferred to a low-protein binding tube and 20 equivalents of dopamine (final concentration: 2.8 mM) (Sigma-Aldrich, H8502) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Developmental Biology 2022Quote: ... As control for iodide uptake and protein-binding cells were also treated with 30mM sodium perchlorate (Nis inhibitor; NaClO4, Sigma-Aldrich)) and 2 mM methimazole (TPO inhibitor ...