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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Human Single stranded DNA binding protein 4 SSBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Then slides were incubated with antibodies specific to acrosin binding protein (ACRPB) (1:100 in PBS) (Sigma; HPA039081, USA), IZUMO1 (1:100 in PBS ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Neuroscience 2021Quote: ... Viral supernatant was transferred into a new sterile 50 mL tube and passed through a low protein binding 0.45 μm filter (SE1M003M00, Millipore) to exclude any cells still present in the supernatant ...
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bioRxiv - Molecular Biology 2020Quote: ... Binding and crosslinking of the GST-Af1521 fusion proteins to Glutathione Sepharose 4B beads (Sigma Aldrich, Saint Louis, Missouri), peptide enrichment (“standard workflow” ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifuged at 20000g for 15mins and filtered through a low-protein binding 0.45µm PVDF membrane syringe filter (Millipore, #SLHVX13NL), the extracts were incubated with 30µl mouse anti-HA agarose beads (clone HA-7 ...
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bioRxiv - Genetics 2021Quote: ... Reversal of TetR fusion protein binding was achieved by addition of 1 μg/ml Doxycycline (final concentration / Sigma-D9891) to mESC culture medium.
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were collected at 42-48 h after transfection and filtered through a 0.45 um low-protein binding filter (Millipore). Patient-derived TCL were infected with lentiviral supernatants containing polybrene (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Cancer Biology 2024Quote: ... conditioned media was isolated and filtered through a 0.4µm low protein binding PVDF Miltex syringe-driven filter (Millipore #SLHV033RS) to remove cellular debris ...
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bioRxiv - Cancer Biology 2024Quote: ... Conditioned media was isolated and filtered through a 0.4µm low protein binding PVDF Miltex syringe-driven filter (Millipore #SLHV033RS) to remove cellular debris ...
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: Human C-reactive protein (Cat. No. C4063) and Human erythrocyte catalase (Cat. No. C3556) were obtained from Sigma Aldrich chemicals ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from human dermal fibroblasts (106-05A,Sigma) transfected with Dharmacon siRNA cocktails as follows ...
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bioRxiv - Microbiology 2020Quote: ... Results were normalized to ng actin using Human DNA standard (Sigma). Primers used for quantification of actin were ...
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bioRxiv - Genomics 2021Quote: ... human Cot-1 placental DNA from Sigma-Aldrich (Oakville, ON, Canada), and target-specific biotin-labeled probes in hybridization solution ...
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bioRxiv - Molecular Biology 2019Quote: ... serially diluted human genomic DNA (0.76−12,500 pg; #D4642, Sigma-Aldrich) was utilized as previously described (53 ...
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bioRxiv - Genetics 2024Quote: ... CpGenome™ Human Methylated & Non-Methylated DNA standard set (Sigma#S8001M) was used to prepare a set of methylation standards (0% ...
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bioRxiv - Neuroscience 2022Quote: Fixation was performed following 24 hrs of recombinant protein treatment using 4 % PFA / 4% Sucrose (Sigma) in 1x PBS at RT for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... prepared for sequencing using KAPA Stranded RNA-Seq Library Preparation Kit (Millipore Sigma, St. Louis, MO). Libraries were prepared using the rRNA-depleted RNA as input for NEBNext Ultra II RNA Library Prep Kit (NEB ...
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bioRxiv - Physiology 2024Quote: Cells were stained with the DNA-binding dyes propidium iodide (PI) and Hoechst 33342 (10 µg/mL, Sigma-Aldrich) to count apoptotic cells by fluorescent microscopy ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were probed with rabbit anti-human Cx43 (1:2000) (Sigma-Aldrich) and rabbit anti-ß-Tubulin antibodies (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Molecular Biology 2019Quote: ... the proteins were concentrated with Amicon Ultra 4 MWCO10kDa (Millipore) to approximately 3 – 4 mL ...
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bioRxiv - Molecular Biology 2019Quote: The protein was concentrated with Amicon Ultra 4 MWCO30kDa (Millipore) at 20 °C at 1650 × g ...
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bioRxiv - Cell Biology 2021Quote: ... 50 µl of Protein G Sepharose 4 Fast Flow (Sigma) slurry were washed ...
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bioRxiv - Microbiology 2020Quote: ... Protein was concentrated using Amicon Ultra-4 30k MWCO (Millipore). 3C protease was added to purified protein in a 1:50 (w:w ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified proteins were concentrated by ultrafiltration (Amicon Ultra-4, Millipore) and diluted in PBS.
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced with 4 mM DTT (Sigma-Aldrich) for 60 min at room temperature and alkylated in the dark using 8 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
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bioRxiv - Systems Biology 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... 3×10-4 M monothioglycerol and 300 μg/ml human transferrin (Sigma Aldrich). Cells were plated in petri dishes (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... and then 4 ng of human urokinase-type PLG activator (uPA, Sigma-Aldrich) was added per well ...
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bioRxiv - Neuroscience 2023Quote: Human iPSCs were grown to 60-80% confluency and dissociated into a single cell suspension by Accutase (Sigma-Aldrich, A6964) treatment for 5 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: Single cell solution was obtained from human normal donor and IPF lungs after enzymatic digestion with 10X Liberase (Sigma, 5401127001) and DNaseI (9003-98-9 ...
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bioRxiv - Physiology 2022Quote: ... or ELISA (ghrelin; Millipore).
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bioRxiv - Neuroscience 2023Quote: ... a single cell suspension was washed with PBS and fixed with 4% paraformaldehyde (PFA; Sigma-Aldrich, 441244-1KG) and 0.1% Saponin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and both organoids and mini-colons were dissociated to single cells using 4 mg/mL Protease VIII (Sigma) in dPBS containing 10 μM Y-27632 on ice for approximately 45 min with trituration using a P1000 micropipette every 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Insulin was measured using the rat/mouse insulin ELISA kit from Millipore (EZRMI-13K), according to the manufacturer’s instructions (Wang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... GFAP was also measured in the supernatant with a sandwich ELISA kits (Sigma NS830) using a 200x dilution ...
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bioRxiv - Physiology 2019Quote: ... P10 and 10 weeks of age using a commercially available leptin ELISA kit (Millipore). Serum free fatty acid levels were assayed in chow-fed or HFHS-fed mothers at gestational day 16 and in the offspring of chow- or HFHS-fed dams at P10 and 10 weeks of age using a commercially available FFA kit (Abcam).
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bioRxiv - Developmental Biology 2022Quote: GH was measured using a mouse/rat growth hormone ELISA kit (Millipore – EZRMGH-45K), based on quantitative sandwich enzyme immunoassay technique ...
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bioRxiv - Microbiology 2023Quote: ... and IL-18 (BMS672TEN) in supernatants were measured using the ELISA Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Neuropeptide Y levels were measured using commercially available ELISA kits (EMD Millipore, EZHNPY-25K).
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...