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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Microbiology 2019Quote: ... virus dilutions were overlaid with 1 ml of Opti-MEM [1% Carboxymethylcellulose (Sigma), 2% FBS ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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bioRxiv - Biophysics 2021Quote: ... Plasminogen (human plasma purified protein, Sigma-Aldrich, Germany) and Fibrinogen (human plasma purified protein ...
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bioRxiv - Biophysics 2021Quote: ... and Fibrinogen (human plasma purified protein, Sigma-Aldrich). Experimental conditions are presented in the table I.
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bioRxiv - Biophysics 2021Quote: Plasminohen (human plasma purified protein, Sigma-Aldric, Germany)
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bioRxiv - Developmental Biology 2019Quote: ... 200nL 0.1µg/µL human noggin protein (Sigma H6416) was injected into the blastocoel of embryos at stage 8 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: To confirm activity of the HIV protease a positive control was run alongside all drug-release experiments using an HIV Protease fluoregenic substrate (Sigma Aldrich Cat. No H0790).
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bioRxiv - Developmental Biology 2019Quote: ... rat anti-E-cadherin (Cdh1) (1:300 dilution, Sigma). Secondary antibodies against sheep ...
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bioRxiv - Cancer Biology 2023Quote: ... monoclonal rat anti-E-cadherin (1:300, Sigma-Aldrich), mouse anti-p120-catenin (1:400 ...
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bioRxiv - Immunology 2019Quote: ... thawed virus was lysed in Triton-X (Sigma; 9002-93-1) and used for p24 measurement at the UCLA CFAR Virology core ...
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bioRxiv - Molecular Biology 2021Quote: ... HIV production was activated by adding Vorinostat (10 μM; Sigma). After 2 days ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Biophysics 2019Quote: ... For lipid mobility measurements inside and outside of Gag assembly sites in mammalian cells 3×105 Jurkat T cells 72 h post-infection with NL4.3 HIV-1 Gag-iGFP virus particles or 24 h post-transfection with NL4.3 HIV-1 Gag-eGFP expressing plasmid were resuspended in 200 µL of Leibovitz’s L 15 medium (Sigma) and incubated with 25 nM of the indicated fluorescent lipid analogue probe for 5 min at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... or human hemoglobin (all proteins were purchased from Sigma). Protein nanorods were prepared using chemically modified human serum albumin.
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bioRxiv - Cancer Biology 2020Quote: Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... influenza virus NP (clones A1 and A3, MAB8251, Sigma-Aldrich, 1:100) and anti-mouse IgG AlexaFluor-488 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg/mL of Pronase E protease mixture (Sigma–Aldrich) was gently added directly and allowed to incubate at 37 °C (2 h) ...
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Neuraminidase inhibitors rewire neutrophil function in vivo in murine sepsis and ex vivo in COVID-19bioRxiv - Immunology 2022Quote: ... LPS (1 µg/mL, E. coli 0127:b8, Sigma-Aldrich), LPS plus Oseltamivir or LPS plus Zanamivir ...
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bioRxiv - Pathology 2024Quote: ... a 1% aqueous solution of eosin Y (Sigma E-6003) was prepared in deionized water and Harris Hematoxylin stain (Lerner Laboratories 1931382 ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Immunology 2024Quote: ... the entire culture medium containing single-cycle HIV-1 viruses was collected and filtered through a 0.45-µm syringe filter (Millex-HV PVDF, Millipore). The filtrate was then aliquoted on ice ...
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bioRxiv - Microbiology 2024Quote: ... the entire culture medium containing single-cycle HIV-1 viruses was collected and filtered through a 0.45-µm syringe filter (Millex-HV PVDF, Millipore). The filtrate was then aliquoted on ice ...
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bioRxiv - Immunology 2023Quote: ... E-64d (Sigma), or 0.5% DMSO ...
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bioRxiv - Cancer Biology 2020Quote: ... and purified human GST protein (5 mg/ml) (Sigma-Aldrich) were incubated for 30min with biotinylated probes (100 pM ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed and cells overlaid with MEM containing 1% carboxymethylcellulose (Sigma), 4% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% BSA and 1% human serum (Sigma) to block Fc receptors ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ml 10 mM EDTA (final: 0.1 mM, Sigma, E-6511), 1 ml 1 M HEPES (final ...
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bioRxiv - Immunology 2020Quote: ... or LPS (0.2μg ml−1, E. coli O55:B5 Sigma-Aldrich) in the presence of M-CSF (10ng ml−1 ...
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bioRxiv - Bioengineering 2023Quote: ... 35 µL protease inhibitor cocktail (1 µM E-64, Sigma Aldrich), and 2.5 µL lysozyme (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... Human Nuclei (1:300, Millipore), Human GFAP (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Human nuclei (1:200, Millipore), Iba1 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... human nuclei (1:1000, Millipore), Islet1 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Biophysics 2020Quote: ... 293T cells were transfected with 2.5 μg of ΔEnv IN-HIV-1 plasmid (DHIV3-GFP-D116G)11 using 10 μg of polyethylenimine (PEI, branched, MW ∼25,000, Sigma-Aldrich). After 20 h ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... The role of mannose receptor was evaluated by incubating cells with HIV-1 in the presence of 100µg/mL BMA (Sigma-Aldrich) or 100µg/mL bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... and J-Lat 11.1 (integrated full-length HIV-1 genome mutated in env gene and GFP replacing Nef) cells were cultured in RPMI-1640 medium (Sigma) supplemented with 10% FBS and 100 μg/ml penicillin-streptomycin at 37 °C in a humidified 95% air-5% CO2 atmosphere ...
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bioRxiv - Biophysics 2021Quote: ... 293T cells were transfected with 2.5 μg of ΔEnv IN- HIV-1 plasmid (DHIV3-GFP-D116G) (31) using 10 μg of polyethylenimine (PEI, branched, MW ~25,000, Sigma-Aldrich). After 20 h ...
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...