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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... MaxiSorp plates were coated with 1 μg/mL HIV-GP120 protein (Sigma-Aldrich). Samples were diluted in an eight-point two-fold dilution series ...
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bioRxiv - Immunology 2021Quote: ... were coated with 50 µL/well of 1 ng/µL solution of fragments 308-331 or 421-438 from HIV gp120 peptides or fragment 579-601 of HIV gp41 (Sigma-Aldrich) in carbonate-bicarbonate buffer (pH 9.6 ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... HIV-1 p24 (mouse, Millipore) and HIV-1 Nef (sheep ...
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bioRxiv - Cell Biology 2023Quote: ... and rat anti-human E-cadherin (EMD Millipore, DECMA-1) at a concentration of 1/200 in a humidified incubator overnight ...
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bioRxiv - Microbiology 2022Quote: ... 10 and 25µg mL−1 of human purified E-cadherin (5085, Sigma-Aldrich, USA) in DMEM or DMEM without FBS as control for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... virus was preincubated with the compound (human (Sigma-Aldrich #L8402) or chicken (Sigma-Aldrich #L4919 ...
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bioRxiv - Microbiology 2022Quote: ... and 25 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) in PBS for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 PR cloning into the pET23a expression vector (Novagen), expression in Escherichia coli strain BL21 Star DE3 PlysS (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cell Biology 2019Quote: ... HIV-1+ or mitoTracker+ and CellTracker+ cells were then detached using accutase (Sigma) and co-cultured at a 1:1 ratio on glass coverslips in 24-well.
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... pronase E 1 mg/ml (Sigma), and DNAse I 50 U/μl (Applichem ...
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bioRxiv - Cancer Biology 2019Quote: ... Cyclin E (1:1000, Sigma Aldrich), V5 (1/500 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA (Sigma E-4378), 1 mM MgCl2 (Mallinckrodt H590) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA (Sigma E-4378), 1 mM MgCl2 (Mallinckrodt H590) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% eosin (H&E) (Sigma). For immunostaining ...
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bioRxiv - Neuroscience 2024Quote: ... 1:10,000 Compound E (Millipore #565790; day 1). On day 3 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were probed with rabbit anti-human Cx43 (1:2000) (Sigma-Aldrich) and rabbit anti-ß-Tubulin antibodies (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... HIV-Tat protein transduction motif (YGRKKRRQRRR) was subcloned into a pET-46 Ek/LIC vector (EMD Millipore #71335). The construct encoding a GST-tagged caltubin was subcloned into a pGEX-4T-1 vector (Amersham #28954549) ...
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bioRxiv - Microbiology 2022Quote: ... and 200 nM of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) for 1 h at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Genetics 2021Quote: ... Proteins were then transfered to an Immobilon-E PVDF membrane (Millipore), which was then washed with 1× PBS containing 0.05% Tween 20 (three washes ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Microbiology 2021Quote: ... and 0.8 µg plasmid expressing HIV-1 gag-pol using X-tremeGENE 9 (Sigma). Media was changed 6 h later and lentivirus containing culture supernatants were collected at 48 and 72 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... HIV-1 Env-pseudotyped lentivirus in growth medium supplemented with DEAE-dextran (Millipore Sigma) was added to the serially diluted serum samples.Medium was removed from cells and replaced with 100 μL medium containing the diluted mouse serum and virus ...
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were infected with HIV-1NL4-3 or HIV-1JRFL (PM1-CCR5) and cultured in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% fetal calf serum (Sigma-Aldrich) ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... Cultured astrocytes were treated with 150 pM HIV-1 gp120 CM (ProSpec-Tany TechnoGene Ltd., Israel) for 12 h before application of 5 or 25 μM KYNA (Sigma-Aldrich, USA) for a further 12 h ...
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bioRxiv - Immunology 2022Quote: ... Commercially available flavivirus E protein-specific mAb 4G2 (EMD Millipore, Darmstadt, Germany), rabbit anti-ZIKV prM and C antibodies (GeneTex ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). Further details are described in the S1 Supplemental Methods.
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bioRxiv - Biophysics 2021Quote: HEK293T cells expressing HIV-1 Gag/Gag(i)mEos2 cultured on poly-l-lysine (Sigma) coated 25 mm round ♯ 1.5 coverslips (VWR ...
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bioRxiv - Microbiology 2020Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). Further details are described in the S1 Supplemental Methods.
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bioRxiv - Microbiology 2022Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). The extension reactions were performed at 37 °C for 40 min and included ∼6 to 1200 fmol of total dNTP ...
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bioRxiv - Microbiology 2022Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). The extension reactions were performed at 37 °C for 40 min and included ∼6 to 1200 fmol of total dNTP ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HIV Reagent Program) followed by an actin mouse monocolonal antibody (1:5000 dilution for 1 h; Millipore #MAB1501R). Nef and actin proteins were visualized with complementary anti-rabbit and anti-mouse secondary antibodies coupled to infrared dyes for quantitative imaging using the LI-COR platform.
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Cancer Biology 2022Quote: ... PBS and 1 μg/mL Human Plasma Fibronectin Purified Protein (Sigma-Aldrich, Cat. #: FC010). 1N NaOH was added to achieve a neutral ∼pH and a thin base layer of 100 μL volume was added to the well of a 48-well tissue culture plate precoated with 1% agarose ...
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bioRxiv - Developmental Biology 2020Quote: ... E-cadherin (RRID:AB_477600, Millipore Sigma, U3254, 1:200), Gata6 (RRID:AB_10705521 ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... virus was treated with alpha-1-antitrypsin (Sigma-Aldrich, SRP6312) at the concentration noted ...
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bioRxiv - Evolutionary Biology 2021Quote: The HIV-derived lentiviral vector pLKO.1 containing shRNAs (TRIM17 MISSION shRNA Bacterial Glycerol Stock, Sigma) that targeted Trim17 (shTrim17-1 ...
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bioRxiv - Immunology 2023Quote: ... was used to perform HIV-1 entry assays The following reagents were used PEI (Sigma Aldrich, Servapore dialysis membranes (3.5 kDa MWCO ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...