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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... ½-log serially diluted influenza viruses in virus growth medium plus trypsin (DMEM, 0.1% BSA, 1 µg/mL TPCK-treated trypsin (Sigma, St. Louis, MO, USA)) were added to confluent monolayers of MDCK-SIAT1 cells (37 ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... Virus was thawed at 37°C and 5μl 10mg/ml polybrene (Sigma H9268) was added to the virus ...
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bioRxiv - Biochemistry 2021Quote: ... Cholesteryl hemisuccinate (CHS) and tobacco etch virus (TEV) protease were from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... virus containing supernatant was harvested and filtered (0.45 μm, Millex-HV Filter; Millipore). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were infected with virus in the presence of 8μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Live filtered virus was used to transduce cells with polybrene (10μg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... the virus was concentrated using Amicon ultra-15 centrifugal filter units (Millipore Sigma) by centrifuging at 3000 rpm and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Virus-containing supernatants were filtered through a 0.45-μm-pore-size filter (Millipore) and supplemented with polybrene (8 μg/ ml ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Microbiology 2020Quote: ... 100 μl of virus preparations were treated with 25 units of Benzonase (Sigma) for 30 min before RNA isolation.
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bioRxiv - Cancer Biology 2020Quote: ... the virus was harvested from the medium filtered by 0.45 mm Steriflipfilter (Millipore). Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cells or virus were lysed with RIPA buffer and protease inhibitor cocktail (Sigma). Protein loaded from viral lysates were normalized to p24 antigen content ...
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bioRxiv - Immunology 2021Quote: ... Virus and cell pellets were solubilized in lysis buffer [10mM iodoacetamide (Sigma-Aldrich), CompleteTM protease inhibitor tablets (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was aspirated and cells resuspended in virus medium containing TPCK-trypsin (Sigma), 20 mM HEPES (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Neuroscience 2023Quote: ... The virus was slowly mixed with silk fibroin solution (50mg/mL, Millipore Sigma) with a ratio of 1:1 through the pipette to avoid generating bubbles ...
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bioRxiv - Immunology 2023Quote: ... virus particles were precipitated by adding 50% (w/v) PEG 6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus containing medium with 8 μg/mL polybrene (Sigma-Aldrich, Cat #H9268) was used in 1:1 dilution with the culture medium to infect SKBR3 and HCC1954 cells ...
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bioRxiv - Neuroscience 2023Quote: ... the virus-containing medium was filtered through Costar Spin-X columns (Sigma #8162) as previously described 31.
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bioRxiv - Bioengineering 2023Quote: ... and Anti-Adeno-associated Virus 9 Antibody clone HL2374 (Millipore Sigma, #MABF2326-25UG). Following three additional washes ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Virus-containing medium was passed through a 0.45 µm filter (cat. #SLHVR33RB, Millipore), aliquoted and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Microbiology 2021Quote: Viral stocks of molecular clones HIV-1NL4-3 and HIV-1NL4-3 BaL (AIDS Reagents Program) were generated by transfecting HEK293T cells using PEI transfection reagent (Sigma, Cat: 408727). Pseudotyped HIV-1VSVg-NL4-3 ...
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bioRxiv - Microbiology 2023Quote: ... J-Lat 10.6 and J-Lat 5A8 previously knocked out for ZAP (12) were transduced with HIV-CRISPR library lentivirus with DEAE-Dextran (final concentration 20 ug/mL, Sigma-Aldrich; D9885) at a multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2021Quote: ... nitrocellulose membranes were incubated with primary antibodies against α-E catenin (1: 1000; Cell Signalling Technologies, catalogue number # 240) and α-tubulin (1: 20 000; Sigma–Aldrich, catalogue number T6074) at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50), total SR proteins (1;1000 ...
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bioRxiv - Bioengineering 2019Quote: ... microtubule associated protein 2 (MAP2) (1:500, Sigma-Aldrich). The cells were subsequently washed 6 x 5 min each with PBS/0,1% Triton-X 100 ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-Glial Fibrillary Acidic Protein (1/1000, Millipore, MAB360). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced using 1 mM dithiothreitol (Sigma-Aldrich) for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Anti-ACTB reference protein (Sigma Aldrich, A2066, 1:10000) antibody was also used ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-microtubule associated protein 2 (MAP2, 1:200, Millipore), mouse monoclonal anti-ankyrinG (AnkG ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM NaVO3) with protein inhibitor cocktail (Sigma®) followed by total protein quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-SR proteins (Millipore, cat#MABE126, 1:2,000 dilution); anti-phosphoepitope SR proteins clone1H4 (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse antiglial fibrillary acidic protein (GFAP) (Millipore, 1:500), Beta-actin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... myelin basic protein (MBP; 1:200; MAB386; Milipore Sigma), Synaptophysin (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was expressed using a pETBlue-1 (Novagen) vector in E ...
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bioRxiv - Neuroscience 2023Quote: ... Thorough washing was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...