Labshake search
Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... calpain substrate peptide or conjugates were incubated with 26.6 nM human calpain-1 (Sigma-Aldrich, C6108) in 50 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (HEPES) ...
-
bioRxiv - Cell Biology 2022Quote: ... per 1 ml fibrinogen at 1.5 mg/mL (plasminogen-Depleted from human plasma, Sigma-Aldrich #341578) in 1x Hanks’ buffered salt solution (HBSS ...
-
bioRxiv - Cell Biology 2023Quote: IWAKI 35mm dishes (12mm glass diameter) were coated with 1:100 Human Fibronectin plasma (Sigma-Aldrich) in PBS and incubated at 4°C overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... otherwise all other protein labelling were using 1% Triton X100 (Sigma-Aldrich) in PBS permeabilization for 10 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... Guanine nucleotide-binding protein subunit alpha-11 (1:1,000; Millipore 05-855R), Guanine nucleotide-binding protein G(q ...
-
bioRxiv - Molecular Biology 2021Quote: ... anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263, Merck Millipore; 1:1000). Ponceau solution was prepared with Ponceau BS (cat# B6008 ...
-
bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
-
bioRxiv - Molecular Biology 2019Quote: ... protein expression was induced by using 1 μg/ml doxcycline (Sigma Aldrich) for 24h.
-
bioRxiv - Cell Biology 2019Quote: ... protein expression was induced by using 1 μg/ml doxcycline (Sigma Aldrich) for 24h and checked by Western blot analysis.
-
bioRxiv - Genetics 2021Quote: ... clarification and benzonase treatment (250 u/1 mg protein, SIGMA E1014-5KU) extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D ...
-
bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
-
bioRxiv - Biophysics 2020Quote: Proteins were buffer exchanged into 1 M aqueous ammonium acetate (Sigma-Aldrich) using P6 Biospin columns (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μg of total protein was added to 7.8 nmol nitrocefin (Sigma) and absorbance at 495 nm measured kinetically for 10 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were probed with anti-thymidylate synthase antibody (Millipore, Cat:MAB4130, 1:500), anti-CDK6 antibody (Cell Signalling ...
-
bioRxiv - Cell Biology 2022Quote: ... protein expression was induced using 1 μg/mL of doxycycline (Sigma, D9891) for 16 h.
-
bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with rabbit anti-HA (1:1,000 Sigma-Aldrich, H3663) or anti-β-actin as loading control (1:10,000 Sigma-Aldrich ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with mouse anti-FLAG (1:5,000 Sigma-Aldrich, F3165) or anti-β-actin (1:5,000 Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... Immunohistochemistry was performed using glial fibrillary acidic protein (GFAP) (1:1000; Millipore) and ionized Ca2+-binding adapter protein (1:1000 ...
-
bioRxiv - Neuroscience 2020Quote: ... anti-MBP (Myelin Basic Protein, rat polyclonal, 1:1000 Millipore MAB 386) or antimouse IgG (Biotin-SP AffiniPure ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-microtubule-associated protein 2 (MAP2) Alexa 555 (1:500, MAB3418A5; Millipore), antimicrotubule-associated protein 2 (MAP2 ...
-
bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Anti-Prosurfactant Protein C (pro-SP-C) antibody (1:4000) (Millipore, #AB3786). Antibodies were incubated in 2% powder milk in TBS pH 7.4 ...
-
bioRxiv - Cell Biology 2022Quote: ... DNA-protein complexes were crosslinked using 1% final concentration of formaldehyde (Sigma) for 10min at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse anti-microtubule-associated protein 2a+b (MAP2, Sigma #M1406; 1:2000), rabbit anti-tyrosine hydroxylase (TH ...
-
bioRxiv - Neuroscience 2023Quote: ... Anti-Myelin Basic Protein antibody [12] (MBP) (Millipore; rat monoclonal, 1:500), Mouse anti-A2B5 (Invitrogen ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were detected with anti-Flag antibody (Sigma, F1804, monoclonal, 1:1,000) or anti-Sso antibody (rabbit antiserum ...
-
bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with 1:50 trypsin (Sigma-Aldrich) and 1:75 and lysyl endopeptidase (Wako) ...
-
bioRxiv - Cell Biology 2023Quote: ... and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich, #D141-100MG). Mitochondria were incubated on ice for 20 minutes and then centrifuged at 20,000 x g for 10 minutes at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 μg/mL doxycycline hyclate (Sigma Aldrich), and the culture was placed in a shaking incubator maintaining 37°C and a 5% CO2 atmosphere ...
-
bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (1:1000, Millipore Sigma, #MAB3402), rat monoclonal anti-CD45 (1:200 ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated with 1:4 trichloroacetic acid solution (TCA, Sigma Aldrich) and washed with acetone ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse anti-glial fibrillary acidic protein (GFAP, 1:1000, Millipore Sigma, USA), anti-NeuN (1:500 ...
-
bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Rabbit polyclonal antibody against protein disulfide isomerase (1:100) (PDI; P7122, Sigma). Secondary antibody Alexa Fluor 555 (A-21422 ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
-
bioRxiv - Neuroscience 2024Quote: ... Mouse Anti-Glial Fibrillary Acidic Protein (1:100, Millipore, Cat# MAB360, RRID:AB_11212597), Mouse Anti-NeuN (1:1000 ...
-
bioRxiv - Microbiology 2021Quote: ... sBHI media controls or Porphyromonas cell-free supernatants (240 µg of total protein) were incubated with 120 µg of human fibrinogen (Sigma-Aldrich, St. Louis, MO). Reaction mixtures were incubated at 37°C under anaerobic conditions or in an atmosphere of 5% CO2 for cell suspension or cell-free supernatants ...
-
bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45µm filter (EMD Millipore), and used for transduction of 4T07 ...
-
bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(-) ...
-
bioRxiv - Cell Biology 2019Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2µg/ml Puromycin (Sigma-Aldrich). After 7 days of selection ...
-
bioRxiv - Cell Biology 2021Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45μm filter (EMD Millipore) and used for transduction of MCF-10A cells in the presence of polybrene (8μg/mL) ...
-
bioRxiv - Neuroscience 2021Quote: Each animal (with DREADD virus injection) received CLZ (0.5 mg/kg, Sigma-Aldrich, dissolved with 0.1% DMSO) or vehicle (sterilized saline with 0.1% DMSO ...
-
bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
-
bioRxiv - Cancer Biology 2022Quote: ... pLVX-EF1α-mKate2-WPRE-Neo virus supplemented with 10 µg/ml polybrene infection/transfection reagent (Sigma-Aldrich) was incubated with PSCs grown in monolayer for 24 h ...
-
ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... virus was also concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). Mice were injected s.c ...
-
bioRxiv - Bioengineering 2021Quote: ... cells were transduced with 4 µL library virus/well along with 8 µg/mL Polybrene (Sigma-Aldrich) aiming for an MOI at 0.3-0.4 (Supplementary Methods and Results) ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then cultivated with the virus in the presence of 4 μg/ml polybrene (Sigma-Aldrich) for 12 hr.
-
bioRxiv - Cancer Biology 2022Quote: ... cells were treated with virus (MOI 0.5-1.0) and 5 µg/mL Polybrene (EMD Millipore, TR-1003). Polyclonal cells were selected for 2 weeks in 250 µg/mL G418 (ThermoFisher Scientific ...