Labshake search
Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... Proteins were detected with antibodies against GFP (Sigma, 1:1000) and FMR polyclonal antibodies (1:1000) ...
-
bioRxiv - Biochemistry 2019Quote: ... and supplemented with SyproOrange Protein Gel Stain 1:500 (Sigma). After incubation on ice for 1.5 h ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μg of total protein and anti-GFP (Sigma SAB4301138) at 1:500 dilution was used ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse anti-microtubule-associated protein 2 (Map2, 1:250, Sigma), rabbit anti-glial fibrillary acidic protein (GFAP ...
-
bioRxiv - Molecular Biology 2020Quote: ... containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma-Aldrich) and protease inhibitors (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: ... recombinant protein was induced with 1 mM IPTG (Sigma – Aldrich) and the culture was transferred to 25°C and 250 rpm for 5 hours ...
-
bioRxiv - Developmental Biology 2022Quote: ... proteins were precipitated using 1 mg of gamma-globulins (Sigma) and 2 ml 20% TCA followed by centrifugation at 2,000 r.p.m ...
-
bioRxiv - Plant Biology 2022Quote: ... Six ml of protein extraction buffer (1 tablet of Sigma Complete™ mini protease inhibitor ...
-
bioRxiv - Cell Biology 2020Quote: ... and RSV F protein (1:500 dilution, 488 conjugated; Millipore). Cultures were mounted using DAPI mounting medium (Vectashield ...
-
bioRxiv - Physiology 2021Quote: ... followed by peroxidase-conjugated Protein G (Sigma P8170; 1:130’000) or peroxidase-conjugated anti-mouse IgG (Fab Specific ...
-
bioRxiv - Neuroscience 2020Quote: ... glial fibrillary acidic protein (GFAP, 1:1000, #SAB4100002, Sigma-Aldrich), NeuN (1:500 ...
-
bioRxiv - Cell Biology 2021Quote: ... Sftpc (1:100, anti Pro Surfactant protein, rabbit, AB3786 Millipore), Ki67 (1:200 ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary antibodies against pan-talin protein (1:1000, Sigma-Aldrich) and actin control (1:2000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
-
bioRxiv - Molecular Biology 2019Quote: ... 4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk ...
-
bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
-
bioRxiv - Biochemistry 2020Quote: ... full length human PCNA (accession no. NM182649) was cloned into pETDuet-1 MCS1 (Novagen) Amp+ to obtain 6× His N-terminally tagged protein ...
-
bioRxiv - Immunology 2019Quote: ... incubated with alkaline phosphatase-conjugated goat anti-human IgG (1 μg/mL, Sigma-Aldrich) for 1 hr at room temperature ...
-
bioRxiv - Developmental Biology 2019Quote: ... Human cells were detected using directly Cy3-conjugated anti-HNA (Millipore, MAB1281C3, 1:100). After washing in TBS ...
-
bioRxiv - Bioengineering 2020Quote: ... anti-human mitochondria antibody Alexa Fluor® 488 conjugate (MAB1273A4, 1:100, EMD Millipore), and DAPI.
-
bioRxiv - Biochemistry 2020Quote: ... then resuspended in 1:500 anti-human IgG antibody conjugated to Cy3 (Sigma #C2571). Fluorescence staining was measured by a BD Fortessa flow cytometer.
-
bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL-1 and 5×10-7 M hydrocortisone hemisuccinate (Sigma). Cells were seeded and expanded for two weeks and subsequently differentiated for another two weeks in the presence of 1.8% DMSO61 (dHepaRG).
-
bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL−1 and 5×10−7 M hydrocortisone hemisuccinate (Sigma). As a control we generated HepaRG cells expressing an inactive HBx null mutant (HepaRG-HBxSTOP ...
-
bioRxiv - Neuroscience 2022Quote: ... The following synaptic antibodies were used: Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543), Chicken anti-human MAP2 (1:1000 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 50,000 human ECs (RBPJ knock-out or shRNA for CXCL2 (pLKO.1; Sigma Aldrich) and respective controls ...
-
bioRxiv - Neuroscience 2022Quote: ... before blotting with the primary antibodies: Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543) and Mouse anti-human GAPDH (1:1000 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Mouse monoclonal anti-human SHC4 (clone 2F5, dilution 1:300) was from Sigma-Aldrich. Rabbit polyclonal anti-human CHORDC1 (NBP1-78304 ...
-
bioRxiv - Bioengineering 2021Quote: ... and 25 μg mL−1 fibronectin from human plasma (Cat. no. F1141, Sigma-Aldrich). Cells have previously been characterized for expression of tight junction proteins ...
-
bioRxiv - Cell Biology 2020Quote: ... Immunofluorescence in human cells was conducted using antibodies against mouse □-tubulin (Sigma, 1:1000), mouse CENP-E (Abcam ...
-
bioRxiv - Microbiology 2020Quote: ... 5 μg mL−1 hemin and 10% (vol:vol) heat-inactivated human AB serum (Sigma). Cultures were sampled daily ...
-
bioRxiv - Microbiology 2022Quote: ... 10 and 25µg mL−1 of human purified E-cadherin (5085, Sigma-Aldrich, USA) in DMEM or DMEM without FBS as control for 1 h at 37 °C ...
-
bioRxiv - Developmental Biology 2022Quote: ... 10 ng/ml human LIF and 1 mM valproic acid sodium salt (Sigma-Aldrich). Starting from Day 4 ...
-
bioRxiv - Cell Biology 2023Quote: ... Human microvascular endothelial cells (HMEC-1) were cultured in MCDB-131 medium (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) ...
-
bioRxiv - Immunology 2023Quote: ... Human tumor necrosis factor alpha (TNFα; Sigma-Aldrich, St. Louis, MO; 1 µg/mL) was added to the cultures for the last 3 d ...
-
Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: ... Coverslips were blocked and permeabilized in staining buffer [1% human serum (Sigma Aldrich, 4552) heat-inactivated at 56 °C for 1 hr ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal antibody against human Integrin alpha-5 (1:100 for immunofluorescence; EMD Millipore; catalogue number MABT201 ...
-
bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
-
bioRxiv - Immunology 2019Quote: ... monocytes were cultured at 1 × 106 cells/ml in RPMI-1640 medium supplemented with 1% human AB serum (Sigma), 20 ng/ml M-CSF (Peprotech) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... GATA 3 (SAB2100898) (rabbit) 1:400 dilution for zebrafish tissue and 1:100 dilution for human tissue (Sigma Aldrich) and and CD44 (SAB1405590 ...
-
bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
-
bioRxiv - Microbiology 2024Quote: ... ELISA plates were blocked for 1 h in AD before addition of assay samples for 1 h followed by incubation with 1/20,000 dilution Goat anti-human Fc-HRP (Sigma). All steps were conducted at 37°C and separated by three washes with PBS-T (0.05% Tween-20) ...
-
bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
-
bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
-
bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
-
bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
-
bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
-
bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...