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Citations for Millipore Sigma :
301 - 350 of 3788 citations for Human Ephrin B2 EFNB2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Molecular Biology 2021Quote: ... with subsequent immunoblotting against either the FLAGepitope tag or TWIN-Strep epitope tag (Anti-FLAG M2, Sigma-Aldrich; THETM NWSHPQFEK antibody, GenScript).
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bioRxiv - Cell Biology 2020Quote: ... and S-tag (EMD Millipore, MAC112, WB: 1:5000). Rat monoclonal antibody ...
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bioRxiv - Physiology 2020Quote: ... Plasma TAG was measured with a colorimetric assay (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich), anti-tubulin # T8660 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated mouse anti-FLAG tag Ab (Sigma-Aldrich) was used to detect Fab/VH binding ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to Flag-tag (F3165) were purchased from Sigma. Antibodies to pT308-Akt (ab38449 ...
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bioRxiv - Plant Biology 2021Quote: ... or western blot using HA Tag Antibodies (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tag (M2) and α-Tubulin (T5168) from Sigma, RFP (600-401-379 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal Flag-tag antibody (Sigma, F3165, 1:1000), rabbit monoclonal HA-tag antibody (CST ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-Flag tag (Sigma cat# F1804, 1:1000), rabbit anti-human actin (Sigma cat#A2066 ...
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bioRxiv - Microbiology 2019Quote: ... The GST tag was cleaved with PreScission Protease (Millipore) and proteins were dialyzed overnight in 50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... against a primary antibody (GST-tag Monoclonal antibody, Novagen), which is attached to GST-tagged TF proteins (Figure S4).
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by thrombin protease (Sigma) in cleavage buffer (20 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Biochemistry 2021Quote: ... GST tag was cleaved by incubating with thrombin (Sigma) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The FLAG tag oligonucleotide was synthesized by Sigma-Aldrich as two complementary single-stranded oligonucleotides (Supplementary Table 1).
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bioRxiv - Genetics 2020Quote: ... rabbit αMyc Tag IgG (1:3000; #06-549, Millipore), rabbit αHSF2BP R2 (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... and S-tag from MBP-enhanced vector pTriEx4 (Novagen); the second insert contained CL Affinity Tag followed by a 90bp linker taken from pl21 plasmid (TriAltus) ...
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Cell Biology 2023Quote: ... TAMRA-alkyne tag (1:5000; Sigma Aldrich, Cat# 900932), TCEP solution (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Antibody for Flag-Tag (A8592) was ordered from Sigma.
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Biochemistry 2022Quote: ... GST tag was then cleaved off using preScission protease (Sigma) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... HSV tag (Sigma-Aldrich H6030, 1:1,000 or 1:2,000), Rpb1-Core (Y80 Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG tag (M2) and α-tubulin (T5168) from Sigma-Aldrich; βIII-tubulin from Biolegend (801201).
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag was cleaved with restriction-grade thrombin (Millipore) overnight on ice in Tris buffer supplemented with 2.5 mM CaCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... The HA tag oligonucleotide strand was synthetized by Sigma-Aldrich as two complementary single-stranded oligonucleotides (Supplementary Table 5) ...