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Citations for Millipore Sigma :
551 - 600 of 3788 citations for Human Ephrin B2 EFNB2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Microbiology 2019Quote: ... the blots were incubated with an anti-His6-tag monoclonal antibody (Novagen, Darmstadt, Germany) at 37 °C for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: ... PVDF membranes were probed with anti-Flag-tag Monoclonal M2-Peroxidase (HRP) antibody (SIGMA) or anti-KLF1 7B2 antibody as was described (13).
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bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
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bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
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bioRxiv - Cell Biology 2021Quote: ... Atg18 was isolated by affinity purification using the Gly6-FLAG3 tag and Dynabeads (Sigma) crosslinked with the FLAG M2 antibody (F1804 epitope ...
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bioRxiv - Microbiology 2020Quote: PfaF (locus tag: PBPRA0221) was cloned into pET28 as an NheI-XhoI fragment (Novagen) as to generate N-terminal 6xHis tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The results were analyzed by immunoblotting using antibodies for the Flag tag (SIGMA, SLCD6338).
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bioRxiv - Cell Biology 2022Quote: ... FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma, MA). EGFP was swapped with mEos3.2 41 in the cortactin-EGFP vector to obtain cortactin-mEos3.2 ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21(DE3) as a fusion protein containing an N- terminal His6-tag (Novagen) and purified by metal-affinity chromatography (Ni-NTA ...
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bioRxiv - Biochemistry 2019Quote: The TAP tag was detected using Peroxidase-anti-peroxidase 1:5000 (Sigma, Lot #103M4822), Pgk1 was detected using mouse anti-Pgk1 1:7500 (459250 ...
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bioRxiv - Physiology 2023Quote: ... TAG levels were then measured using the Serum Triglyceride Determination Kit (TR0100-1kt, Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal ANTI-FLAG® M2 antibody used in Cut&Tag assay was from Sigma. Mouse CD4 T Cell Isolation Kit used for the enrichment of CD4+ T cells was from Stem cell ...
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bioRxiv - Biochemistry 2023Quote: ... REC114 variants were cloned as N-terminal Strep-tag fusions into pRSFDuet-1 (Novagen) and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2h with antibodies recognizing the FLAG-tag (F-1804; Sigma-Aldrich), Strep-tag (NBP2-41073 ...
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bioRxiv - Physiology 2023Quote: TAG and total cholesterol evaluation were processed according to the manufacturer’s instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
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bioRxiv - Genetics 2024Quote: ... These antisense DNA probes were synthesized with Biotin-TAGs at the 5’ ends (Sigma) and 100 µM probes were pooled in equimolar ratios ...
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bioRxiv - Molecular Biology 2024Quote: ... and a C-terminal His6-tag was cloned into the expression vector pET21b (Novagen). The MALT1(PCASP-Ig3)339–719-his construct was transformed into Escherichia coli strain T7 express competent cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... TAG content was quantified using the High Sensitivity Triglyceride Assay Kit (Sigma-aldrich MAK264), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Microbiology 2020Quote: ... Human serum-supplemented medium was made through addition of complete human serum (Sigma Aldrich) into CDM to a final concentration of 5% (v/v) ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in an equal volume of human serum from human male AB plasma (Sigma) and incubated for 16 h at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10 % heat-inactivated human serum from human male AB plasma (Sigma-Aldrich), 2.5 μM zoledronic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... His-PBP1B containing fractions were pooled and treated with 2 U/mL of thrombin (Novagen) for 20 h at 4 °C during dialysis against dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
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bioRxiv - Microbiology 2021Quote: ... PfArlH or biotinylated PfArlI were subsequently mixed with HIS-Select® Nickel Affinity resin (SIGMA) while lysate of cells overexpressing Strep-SaArlI was mixed with Streptactin column material (IBA GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... DTT was added to a final concentration of 0.5 mM and His-SUMO protease (Sigma) was added to a concentration 100-fold lower than the total protein concentration ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...