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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... mitomycin C (MMC, Sigma) was dissolved in sterile saline solution on the day of injection to yield 0.5 mg/ml solution ...
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bioRxiv - Neuroscience 2023Quote: ... c-Jun shRNA (Sigma, mission shRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Mitomycin C (Sigma; #M4287), AZD1056 (kindly provided by AstraZeneca) ...
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bioRxiv - Molecular Biology 2023Quote: ... ara-c (Sigma #C1768) was added to a final concentration of 0.5 µM to inhibit proliferation of any contaminating glial cells ...
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bioRxiv - Cell Biology 2023Quote: ... C-Nap1 (Millipore; MABT1353) at 1/500 dilution ...
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bioRxiv - Bioengineering 2020Quote: ... Multiplexed chemokine and cytokine analysis was performed using Milliplex MAP Rat Cytokine/Chemokine Magnetic kit (Millipore Sigma) and analyzed using a MAGPIX Luminex instrument (Luminex) ...
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bioRxiv - Cell Biology 2021Quote: ... were coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation). Cells were seeded at a density of 315,000 cells per insert in 250 µL HITES media ...
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bioRxiv - Genomics 2021Quote: ... Serum samples obtained from vaccinated and non-vaccinated individuals were stored at −20°C until tested treated with receptor destroying enzyme (RDE) (Sigma C8772, diluted 1:4), for 16 h prior to heat inactivation (30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Agarose beads were heated at 95°C for 5 min in SDS sample buffer containing 5 % β-mercaptoethanol (Sigma). After SDS-PAGE and transfer to a nitrocellulose membrane with a 0.2 μm pore size (RioRad) ...
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bioRxiv - Systems Biology 2020Quote: ... Separations were performed at 40°C on a Discovery BIO wide pore C5-5 column (15 cm × 2.1 mm × 5 μm) from Millipore-Sigma with a paired Supelguard (2 cm × 4 mm × 5 μm ...
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bioRxiv - Microbiology 2022Quote: ... 1ml of culture was incubated for 5 minutes at 37°C with 5 μg/ml of nile red (Sigma) to stain the membrane and the cell wall was labeled with 1 μg/ml of an equal mixture of vancomycin (Alfa Aesar ...
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bioRxiv - Microbiology 2020Quote: ... 5% human serum (Sigma), Penicillin-Streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... 5% human serum (Sigma) and 50% virus solution in RPMI media (Gibco) ...
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bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
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bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
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bioRxiv - Microbiology 2024Quote: ... 5 and 7 dpi were quantified using the Milliplex MAP Porcine Cytokine/ Chemokine Magnetic bead kit (Millipore) and the BioPlex Magpix Reader (Bio-Rad) ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... o/n at 4 °C + 2 hr at 37°C] (Sigma; C3865, USA), CD73 (1:100 in PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were treated at 37°C with 0.5U/ml neuraminidase (C. perfringens, Sigma) to remove sialic acid ...
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bioRxiv - Genetics 2022Quote: ... The lysate was centrifuged at 4,000 x g for 5 min at 4°C and the protein pellet was resuspended in Digestion Buffer (10% 2,2,2-Trifluoroethanol [Sigma; #96924] in 100 mM HEPEs pH 8.5). Protein was quantified with BCA (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... NMDA receptor antagonist AP-5 (2 mM; Sigma Aldrich, St. Louis, MO), and AMPA receptor antagonist CNQX (0.4 mM ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... nicotinic acetylcholine receptor antagonist mecamylamine (5 mg kg−1,M9020, Sigma-Aldrich) and muscarinic acetylcholine receptor antagonist atropine (5 mg kg−1 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Bioengineering 2020Quote: The secreted proteins from different strains were analyzed for cellulase and -mannanase activity as well as reducing sugar yield at three different condition of temperature (30°C, 40°C and 50°C) using carboxymethyl cellulose or locust bean gum as a substrate (Sigma-Aldrich Co.). The activity assay was determined by using 3,5-dinitrosalicylic acid (DNS ...
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bioRxiv - Immunology 2023Quote: ... were slowly (-1°C/min) frozen down to -80°C in a isopropanol alcohol-walled freezing unit (Nalgene Mr. Frosty: C-1562 Sigma-Aldrich, USA) before being transferred to 25-vial boxes in liquid nitrogen.
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested by centrifugation (15,000 x g, 40 min, 4°C) and the supernatant was filtered (0.2 μm, Millipore) before concentration on a tangential flow filtration system equipped with a 30 kDa cut-off filter membrane (Sartorius) ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then filtered for 5 min at 14,000 x g at 4°C using a 0.22 uM low-binding Durapore PVDF filter (Millipore).
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bioRxiv - Biophysics 2021Quote: ... stalled ribosome complexes were filtered for 5 min at 14,000 x g at 4°C using a 0.22 uM low-binding Durapore PVDF filter (Millipore).
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bioRxiv - Microbiology 2019Quote: ... followed by clarification (10 min, 21,000 g, 4°C) and filter-sterilization through a 0.22 µm Spin-X filter (Sigma). Samples were stored at -80 °C until LC-MS analysis ...
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bioRxiv - Microbiology 2020Quote: ... All urine samples were first clarified from solid contaminants by centrifugation at 2,000 x g for 10 minutes at 4°C and then filtered through a 0.45 μm filter (Millipore) to clear the urine of any bacterial or fungal contamination ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated O/N at 4°C with primary antibodies diluted in 0.1% Triton X-100 (Sigma) blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... and treated with the pre-warmed extraction buffer (37 °C; 0.125% Triton X-100 (X100-500ML, Sigma-Aldrich) and 0.4% glutaraldehyde (G7526-10ML ...
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bioRxiv - Molecular Biology 2022Quote: ... Large aggregates were removed by centrifugation at 4,000 x g at 4°C for 15min and filtration (0.22 μm, Millipore). Refolded Leptin was then purified with IMAC (His-Trap FF ...
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bioRxiv - Genetics 2019Quote: Small-interfering RNAs (siRNA) targeting human C/EBPβ were purchased from Sigma Aldrich (St Louis, MO, USA). siRNA ID ...
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bioRxiv - Genomics 2023Quote: ... Thawed PBMCs were quenched by adding 12 mL 37°C pre-warmed human serum (Sigma Aldrich, # H4522) and were centrifuged at 500 x g for 5 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... were incubated at 4 °C overnight with 5 μg/mL anti-FLAG (Sigma-Aldrich, F1804) in 200 mM NaHCO3 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained at 37°C and 5% CO2 on poly-L-ornithine-coated (Sigma) and laminin-coated (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were denatured for 5 min at 80°C in hybridisation buffer (70% formamide (Sigma), 2.14mM MgCl2 ...
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bioRxiv - Developmental Biology 2021Quote: ... were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449, Millipore), 5ug H2AK119ub (8240 ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were denatured for 5 min at 80 °C in hybridization buffer (70% formamide (Sigma), 25 mM MgCl2 ...
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bioRxiv - Genomics 2021Quote: K562 cells were maintained at 37°C under 5% CO2 in RPMI-1640 medium (Sigma) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... T cells were cultured at 37°C and 5% CO2 in RPMI 1640 (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... at 100°C for 5 min in the presence of 2.5% β-mercaptoethanol (Sigma-Aldrich). Equal amounts of protein (40 µg ...
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bioRxiv - Microbiology 2023Quote: ... congolense IL3000 was cultured at 34°C with 5% CO2 in MEM medium (Sigma M0643) containing 20% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were maintained at 33°C and 5% CO2 in DMEM (Sigma-Aldrich, ref. D5671) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Milliplex magnetic bead cytokine/chemokine plates (Millipore) were prepared according to manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... The protein precipitate was collected on a GF/C glass microfiber filter (Whatman, Sigma Aldrich) and then mixed with EcoScint (Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing grade-modified trypsin (Sigma). Protease digestion was stopped by addition of trifluoroacetic acid to 0.5% and precipitates were removed by centrifugation ...
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bioRxiv - Biophysics 2020Quote: ... The purified protein was incubated and for 1h at 37°C with bovine thrombin (Sigma) in a 1000:1 molar ratio ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing-grade modified trypsin (Sigma). To purify the peptides ...
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bioRxiv - Biochemistry 2020Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...