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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Normal Human Dermal Fibroblasts (NHDF) were purchased from Sigma (C-12302, Sigma). Cells were cultured in EMEM (ECB2071L ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with anti-human Fab (Millipore Sigma) diluted 1:500 in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and purified human GST protein (5 mg/ml) (Sigma-Aldrich) were incubated for 30min with biotinylated probes (100 pM ...
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bioRxiv - Microbiology 2021Quote: ... gondii tachyzoites were passaged onto Human foreskin fibroblasts (HFFs; ATCC, SCRC-1041) monolayers at 37 °C and 5% CO2 in DMEM (Sigma, D6546) supplemented with 10% FBS (BioSell FBS.US.0500) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... calcein (C, >95%), D-cycloserine, fluorescein (F, 95%), fluorescein-5-isothiocyanate (FITC, 90%) and TRITON® X-100 were purchased from Sigma-Aldrich. Ethanol (for UV spectroscopy ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Immunology 2020Quote: ... CVLs were centrifuged at 1,800rcf for 1 hour at 4°C then cleared using Spin-X® Tubes centrifuged at 16,000rcf for 30min at 4°C (Sigma-Aldrich, Lyon, France), aliquoted ...
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bioRxiv - Neuroscience 2021Quote: ... These coverslips were left to grow at 37°C and 5% CO2 and were treated with 2 µl Ara-C (Sigma) on day in vitro 1 (DIV1 ...
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bioRxiv - Cell Biology 2021Quote: ... at 45°C for 5 min, following with overnight digestion with Lys-C (129-02541, Wako Chemicals) and trypsin (T6567, Sigma). TiO2 beads (5010-21315 ...
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bioRxiv - Cell Biology 2023Quote: ... mESCs were grown at 37°C and 5% CO2 on Mitomycin-C inactivated feeder cells (DR4-mouse embryonic fibroblast) plated on 0.1% gelatin (Sigma-Aldrich) in serum/leukemia inhibitory factor (LIF ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained at 5% CO2 and at 37°C and were split at full confluency (at 5% CO2 and at 37°C) using trypsin ethylenediaminetetraacetic acid solution (SIGMA) and phosphate-buffered saline (PBS ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Genetics 2021Quote: ... the resin was washed 5 times with ice cold DOC buffer and the bound protein eluted by 15 minute 70°C incubation in 5% w/v sodium dodecyl sulphate (SDS, Sigma). The eluted protein was reduced with 10 mM TCEP and alkylated using 20 mM Iodoacetamide ...
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked overnight at 4°C in 5% BSA (Sigma) then probed using pERK and tERK antibodies (Cell signalling ...
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bioRxiv - Cell Biology 2020Quote: ... for 5 min and permeabilised using -20°C methanol (Sigma Aldrich) for 2 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Rabbit α-c-Fos (1:750, Millipore PC38, Ab-5 RRID:AB_2314421). After primary antibody incubation and washing 3 times with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... overnight at 4°C and 5 µg/ml laminin (Sigma Aldrich) for 2h at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-surfactant associated protein C (SFTPC, 1:1000, Millipore, AB3786), goat anti-podoplanin (PDPN ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal Prosurfactant Protein C (1:200 pH9, AB3786, Merck Millipore), mouse monoclonal TTF-1 (clone SPT24 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Genomics 2023Quote: ... ∼1 x 106 HepG2 cells were treated with vitamin C (Sodium L-ascorbate, Sigma) at concentrations of 30 ...
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bioRxiv - Biochemistry 2020Quote: ... c (Sigma, aerobically grown S ...
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bioRxiv - Immunology 2020Quote: ... DH10β and STBL3 for propagation of plasmids were cultured at 37°C C (30°C C for STBL3) in LB broth (Sigma-Aldrich) with shaking at 220 rpm.
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bioRxiv - Developmental Biology 2020Quote: ... then manually passaged as single cells following treatment with Accutase (5 minutes, 37°C) or Accumax (10 minutes, 37°C; Sigma Aldrich) at clonal density into Matrigel-coated 24-well tissue culture plates (Corning) ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were grown at 37 °C in 5 % CO2 for two days before proliferation was inhibited with mitomycin C (Sigma-Aldrich).
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bioRxiv - Genomics 2023Quote: ... Cells were grown on gelatin-coated plates under standard cell culture conditions (37°C, 5% CO2, humid) on a ‘feeder’ layer of SNLP mouse fibroblasts mitotically inactivated by Mitomycin-C (Sigma Aldrich). mESCs were routinely passaged at ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Physiology 2023Quote: ... 200μg protein was incubated with anti–c-Myc affinity gel (EZview™ Red Anti-c-Myc Affinity Gel, #E6654, Sigma-Aldrich) overnight ...
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bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 µg total protein was denatured for 5 min at 70°C in 1X Laemmli Buffer (Sigma Cat # S3401-10VL), resolved on SDS-acrylamide gels and electroblotted to nitrocellulose ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... and rested overnight at 37°C/5%CO2 in RPMI-1640 medium supplemented with 10% heat-inactivated human AB serum (Sigma Aldrich, Missouri, USA), 2 mM L-glutamine and 1 mM sodium pyruvate before infection ...
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bioRxiv - Cell Biology 2020Quote: ... Both naïve ESCs and D-EPSCs were cultured at 20% O2 and 5% CO2 at 37°C on mitomycin C (MMC; Sigma-Aldrich M0503) inactivated mouse embryonic fibroblast (MEF ...
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bioRxiv - Immunology 2021Quote: ... Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore). Cytokines in mouse BALF were analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against some cytoskeletal proteins such as vimentin (1:50 in PBS; o/n at 4°C + 2 hr at 37°C) (Sigma; V6630, USA), pericentrin (1:1000 in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Cultures were treated with 5-10 μg/mL mitomycin C (Sigma-Aldrich) in serum-free media for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 5 μg/ml of mitomycin C (S8146, Sigma). At time 0 ...
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bioRxiv - Neuroscience 2021Quote: ... were maintained at 37 °C and 5 % CO2 in DMEM (Sigma, D6546) supplemented with 10 % (v/v ...
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bioRxiv - Developmental Biology 2022Quote: ... for 5 min at 37 °C and washed in IMDM media (Sigma) with an osmolarity of 293 mOsm ...
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bioRxiv - Bioengineering 2023Quote: ... and blocked overnight at 4 °C in 5% donkey serum (Sigma-Aldrich). For the proliferation study ...
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bioRxiv - Immunology 2024Quote: ... at 37⍰°C supplemented with 5 mM uridine and 5 mM uracil (u/u, both Sigma) for the A1160 ΔpyrG strain ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellet was washed with −20°C cold acetone (Sigma-Aldrich), vortexed ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
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bioRxiv - Immunology 2024Quote: ... Protein extracts were immunoprecipitated with a PP2A C-specific antibody (1D6; Millipore). An appropriate phosphorylated peptide (amino acid sequence K-R-pT-I-R-R ...