Labshake search
Citations for Millipore Sigma :
301 - 350 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2023Quote: ... and His-Bind Purification kit (Novagen, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... L-histidine (L-his, Sigma-Aldrich #H8125), L-ornithine (L-orn ...
-
bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
-
bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
-
bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
-
bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
-
bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... thirty mice with colitis were obtained and randomly assigned into three groups (n =8) including the control group (Control) without EA (EA, Sigma-Aldrich,CAS141-43-5) supplements and two treatment groups that were supplemented with 500μM EA (LowEA ...
-
bioRxiv - Microbiology 2021Quote: ... groups of mice received 10 mg/kg clindamycin (Sigma-Aldrich) via intraperitoneal injection ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 embryos per group were collected in TRIreagent (Sigma Aldrich), homogenized using a tissue disruptor (MP-FastPrep-24 ...
-
bioRxiv - Microbiology 2020Quote: ... mouse anti-flavivirus group antigen (1:800, MAB10216, Merck Millipore) and goat anti-Iba1 (1:300 ...
-
bioRxiv - Biochemistry 2022Quote: ... all other chemicals were obtained from Sigma-Aldrich (Merck Group) or Thermo Fisher Scientific.
-
bioRxiv - Bioengineering 2022Quote: ... Z group removing agents lauroyl peroxide (LPO; 97%; Sigma-Aldrich) and hydrogen peroxide (H2O2 ...
-
bioRxiv - Physiology 2021Quote: ... The butyrate-treated group receives sodium butyrate (Sigma-Aldrich, 303410) at a 2% concentration in filtered drinking water ...
-
bioRxiv - Physiology 2021Quote: ... pH 8.0 (adjusted by NH4OH, Sigma Aldrich, Merck group, Poland) as the mobile phase with a flow rate 0.4 ml min−1 and run time 10 min ...
-
bioRxiv - Microbiology 2022Quote: ... and amine group by D-amino acid oxidase (Sigma Aldrich). Hydrogen peroxide was reduced to H2O by horseradish peroxidase (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... Quercetin (10−5 M, 00200595; Sigma-Aldrich/ Merck group, USA) 36 ...
-
bioRxiv - Cell Biology 2022Quote: ... and DTNB (2.5 mM, D8130; Sigma-Aldrich/Merck group, France) was diluted in DMEM ...
-
bioRxiv - Cell Biology 2020Quote: HEK293 cells were cultured in DMEM-F12 (cat n. D6421, Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
-
MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: HEK293 cells plated on covered with Poly-D-Lysine (P0899, Sigma-Aldrich) coverslips were co-transfected with pEZY BIFC N-Venus and C-Venus plasmids 48 hours after plating ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.8×105 HEK293 cells were seeded on poly-L-ornithine (Millipore Sigma) pre-coated 12 mm coverslips ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 cells were grown in DMEM 4.5 g/L glucose (D6429, Sigma) supplemented with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... while HeLa and HEK293 cancer cells were maintained in DMEM (Sigma- Aldrich). Media was supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Biophysics 2023Quote: ... Stable HEK293-6E cell lines were maintained in F17 media (Sigma Aldrich) supplemented with Kolliphor p188 (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
-
bioRxiv - Neuroscience 2020Quote: ... The slides were then incubated in blocking Buffer B2 (10% Sheep serum [Sigma S3772] in Buffer B1) at room temperature for a minimum of one hour ...
-
bioRxiv - Molecular Biology 2020Quote: ... Human HEK-293T cells were grown in Dulbecco’s Modified Eagle’s high glucose medium with HEPES (Sigma-Aldrich®, D6171) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Genetics 2021Quote: Human embryonic kidney (Hek) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
-
bioRxiv - Cell Biology 2021Quote: ... 6μl of 10μM siRNAs targeting human KLC1 or KLC2 were mixed with 12μl HiPerFect in high-glucose DMEM medium (Sigma-Aldrich) and combined with 1×105 cells per well of a 6-well plate ...
-
bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney epithelium 293T (HEK293T) cells and Hela cells were cultured in DMEM-high glucose (Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: Human adenocarcinoma alveolar basal epithelial cells (A549, ATCC) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
-
bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
-
bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
-
bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The pure proteins were concentrated using a 3000-high molecular weight cutoff centrifugal filter (Merck-Millipore). The concentrated proteins were then subjected to size-exclusion chromatography using a Superdex-75 GL column (GE healthcare pre-equilibrated with 25 mM Tris-HCl 6.8 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
-
bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
-
bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
-
bioRxiv - Biophysics 2021Quote: ... The protein was specifically labeled via the histidine linker on the N-terminal side of the protein with the dye Atto647N containing a Ni-NTA functional group which was also purchased from Sigma Aldrich (catalogue number 02175-250UG-F). First ...
-
bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
-
bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
-
bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
-
bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
-
bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
-
bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
-
bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
-
bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...