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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... with the same combination of primary and secondary antibodies: mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and rabbit anti-HA tag antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Microbiology 2022Quote: ... and HOS-ACE2/TMPRSS2 cells (HOS cells stably expressing human ACE2 and TMPRSS2)32,33 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... and HOS-ACE2/TMPRSS2 cells (HOS cells stably expressing human ACE2 and TMPRSS2)36,37 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: Cell lines (HEK293T, HEK293, 3C11, 143B, MDCK-SIAT1, MDCK-PR8) were maintained in DMEM (Sigma Aldrich) supplemented with 10% FCS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
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bioRxiv - Bioengineering 2021Quote: ... for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG; Sigma Aldrich) before seeding cells onto the 8-well plates ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were seeded in 8-well Nunc Lab-Tek II chamber slides (0,7 cm2, Sigma) at a density of 70.000 cells/cm2 ...
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bioRxiv - Immunology 2020Quote: 2×105 HEK293 were seeded onto 24well plates previously pre-coated with poly-L-lysine (Sigma), and infected with 2×105 ...
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bioRxiv - Immunology 2020Quote: ... this mixture was applied to mammalian FreeStyle™ HEK293-F cell culture (EMD-Millipore, Darmstadt, Germany) at a density of 1 million cells per milliliter ...
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bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded onto glass coverslips coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation) at a density of 80,000 cells and 40,000 cells for the PDGFRα homodimer cell line and PDGFRβ homodimer cell line ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Developmental Biology 2020Quote: Cells were seeded onto glass coverslips coated with 5 μg/mL human plasma fibronectin purified protein (Millipore Sigma #FC010). At ~90–100% confluency ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
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bioRxiv - Developmental Biology 2021Quote: ... plates were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Sigma-Aldrich, 5µg/ml in PBS, 1h at RT).
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bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Cell Biology 2023Quote: Chromosomes were labeled by either injecting mRNA encoding human H2B-mCherry or H1 protein purified from calf thymus (Sigma) labeled with AlexaFluor 568 or 647 as described previously (Bun et al. ...
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bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
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bioRxiv - Biochemistry 2023Quote: Proteins guided towards high cysteine content were transformed into and expressed in Rosetta-gami B(DE3) Competent Cells (Novagen 71137). The 1 mL and 50 mL scale protein purification protocols were otherwise followed.
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Genetics 2020Quote: ... each group of flies was covered by 100 uL hexane (Sigma-Aldrich #139386-500ML) containing 50µg/mL hexacosane (Sigma-Aldrich #241687-5G ...
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bioRxiv - Developmental Biology 2019Quote: ... The second group was injected with DMSO (dimethyl sulfoxide) (Sigma, Oakville, Ontario, Canada, #472301) vehicle ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: ... Single-stranded DNA oligonucleotides with amine or thiol functional groups were purchased from Sigma. Before annealing the DNA scaffold ...
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bioRxiv - Biochemistry 2019Quote: Accessible amino groups in HSA were detected using fluorescamine (Sigma-Aldrich, St. Louis, USA) as described 54 ...
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bioRxiv - Genetics 2021Quote: ... nine fish per group were anesthetized with tricaine methasulfonate (MS-222, Sigma-Aldrich, USA) and immersed in 10 mg/ml epinephrine (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: To assess microstructural differences between AVs from different groups Masson’s Trichrome staining (Sigma-Aldrich) was used according to manufacturer instructions ...
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bioRxiv - Physiology 2020Quote: ... The experimental group was supplemented with 0.1M dexamethasone diluted in DMSO (dimethyl-sulphoxide, Sigma) to a final concentration of 0.1µM ...
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bioRxiv - Physiology 2020Quote: ... The tendons were incubated at 37°C with elastase (Sigma Group, Malmö, Sverige, E1250) as follow ...
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bioRxiv - Systems Biology 2021Quote: ... Sulfhydryl groups were alkylated with 2 µL of 0.5 M iodoacetamide (IAA; Sigma-Aldrich), vigorous vortexing ...
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bioRxiv - Biochemistry 2022Quote: ... Mice in the Bap group were fed Bap (Sigma, City of Saint Louis, USA), solubilized in corn oil at a dose of 25.3mg/kg ...
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bioRxiv - Genetics 2022Quote: ... each group of flies was covered by 100 uL hexane (Sigma-Aldrich #139386-500ML) containing 50μg/mL hexacosane (Sigma-Aldrich #241687-5G ...
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bioRxiv - Immunology 2019Quote: ... using mouse monoclonal anti-flavivirus group antigen clone D1-4G2-4-15 (EMD Millipore) for detection of ZIKV E and mouse anti-Zika NS1 mAb (BioFront Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells from 3 mice per group were pooled and cultured in α-MEM (Sigma) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... and phosphatase and proteinase inhibitor cocktails (P5726 and P2714; Sigma-Aldrich/Merck group, USA), and scrapped ...
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bioRxiv - Cell Biology 2022Quote: ... 3 groups treated with a combination of 20 μM Antimycin A (A8674, Sigma-Aldrich) and 2 μM Oligomycin (75351 ...
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bioRxiv - Neuroscience 2023Quote: ... alkylation of the free SH-groups was performed Iodoacetamide (IAM) (20mM, Sigma-Aldrich, I1149) at 23 °C for 45 min in the dark ...
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bioRxiv - Plant Biology 2023Quote: ... Malathion (Malathion, PESTANAL®, analytical standard, Sigma-Aldrich, Merck Group, St Louis, MO, USA) was applied at 1000LgLha−1 of active ingredient ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides containing 2’TBDMS protecting groups were dissolved in 115 µL DMSO (Sigma-Aldrich) at 65 °C ...
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bioRxiv - Bioengineering 2023Quote: ... was methacrylated via the esterification of monomeric hydroxyl groups with methacrylic anhydride (Sigma-Aldrich) and then lyophilized for storage ...
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bioRxiv - Microbiology 2024Quote: ... amine groups are formed on the cantilevers using 3-(aminopropyl)trimethoxysilane (APTMS; Sigma-Aldrich). In the second step ...
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bioRxiv - Bioengineering 2024Quote: ... islets were placed in groups of 10 on a poly-L-lysine (Sigma, P4707) coated petri dish filled with KHB ...
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bioRxiv - Neuroscience 2024Quote: ... In group 2 fresh cell culture medium containing 192IgG-saporin (Millipore Temecula, CA, USA) (100 ng/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were randomized into either a “vehicle control” group (0.5 methylcellulose, Sigma-Aldrich #M0430) or a PRMT5 inhibitor “GSK595 treatment” group (50 mg/kg) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-flavivirus group antigen antibody (clone D1-4G2-4-15, Sigma-Aldrich, 1:300), anti-dsRNA (SCICONS ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...