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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... A peptide challenging assay was conducted using PrEst Antigen ONECUT2 (Sigma APrEST86051) with a 5:1 ratio of peptide to ONECUT2 antibody ...
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bioRxiv - Neuroscience 2023Quote: ... and subjected to antigen retrieval using citrate buffer pH6.0 (C9999, Sigma-Aldrich). Sections were blocked with 5% normal donkey serum (in 0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to antigen retrieval in citrate buffer (pH 6.0, Sigma #C9999) for 20 min in a steamer ...
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bioRxiv - Microbiology 2023Quote: ... antigen retrieval was completed by boiling samples in citrate buffer (Millipore Sigma) for 5 minutes followed with removal of heat and an additional 15-minute incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... The virus was propagated on VeroE6-TMPRSS2 cells using DMEM (Sigma) supplemented with 2% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... virus was harvested and purified using an iodixanol gradient (Optiprep, Sigma). For our 7-variant pool ...
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bioRxiv - Neuroscience 2021Quote: ... Virus or Tracer was loaded into mineral oil (Sigma-Aldrich, M3516)-filled glass pipette (WPI ...
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bioRxiv - Microbiology 2021Quote: ... virus was concentrated using 100,000 MW cut-off Centricon concentrators (Millipore).
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bioRxiv - Immunology 2019Quote: ... thawed virus was lysed in Triton-X (Sigma; 9002-93-1) and used for p24 measurement at the UCLA CFAR Virology core ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... we added concentrated virus and 5 μg/ml polybrene (Sigma-Aldrich) to MCF10A cells in growth media ...
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bioRxiv - Immunology 2022Quote: ... virus supernatant was filtered through a 0.44 um PVDF membrane (Millipore), incubated with Lipofectamine 3000 and PLUS reagent as above ...
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bioRxiv - Microbiology 2023Quote: ... the virus containing medium plus 8 µg/ml polybrene (Sigma #H9268) and the infected 293T cells were co-cultured with iSLK cells ...
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bioRxiv - Cell Biology 2023Quote: ... the virus was mixed with fully supplemented DMEM (Sigma-Aldrich, D6429) and 8 μg/ml Polybrene (Sigma-Aldrich ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The remaining lysate was then incubated with 25 μL (1 μL/2 OD600 nm of cells) of monoclonal anti-FLAG M2 (Sigma #F1804) at 4°C with rotation for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 1-2 days later and the lysates were immunoprecipitated using anti-FLAG M2 affinity resins (10 μl per plate, Sigma Inc.). TNKS antibodies were as described (No ...
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bioRxiv - Cell Biology 2019Quote: ... The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads, Sigma-Aldrich) previously washed in Rac buffer (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
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bioRxiv - Biochemistry 2021Quote: ... 400 μg of each lysate was diluted to 2 mg/ml with lysis buffer and incubated with 10 mM TCEP (646547, Sigma-Aldrich) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: Polyacrylamide gel slices containing bEV and sEV protein lysates were resolved in tris (2-carboxyethyl) phosphine (10 × 10-1 M; Sigma- Aldrich) with ammonium bicarbonate (50 × 10-1 M ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... Each lysate was subjected to immunoprecipitation for 2 h or overnight with anti-FLAG M2 affinity agarose affinity gels (Sigma-Aldrich) or anti-HA magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... The lysate was benzonase (Millipore, 70664) treated for 30 min ...
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bioRxiv - Epidemiology 2019Quote: ... The antigen was prepared from cultures grown in Probumin media (Millipore, Billeria, MA) and centrifuged at 349xg for ten minutes at room temperature to remove dead bacteria ...
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bioRxiv - Genomics 2021Quote: ... Antigen retrieval was performed in 1x Tris EDTA pH 9.0 (Sigma Aldrich, SRE0063) in a Tinto Retriever Pressure Cooker (BioSB ...
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bioRxiv - Bioengineering 2019Quote: ... A plastic staining jar with tissue slides immersed in Antigen Retrieval Solution (Sigma 10X Tris-HCl buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Antigens were mixed with SAS adjuvant system (Sigma-Aldrich, St. Louis, MO, USA) to reach a final concentration of 0.05 mg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... Antigen retrieval was performed by incubating slides in 1X citrate buffer (Sigma-Aldrich) at 95°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... were coated with antigen (0.5 μg/mL) in Carbonate Coating Buffer (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... sLea and sLex antigens were detected with mAbs MEM-158 (Sigma-Aldrich France), 7-Le (Sigma-Aldrich France) ...
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bioRxiv - Cell Biology 2020Quote: ... rats were intraperitoneally injected with 100 μg ovalbumin antigen suspension (Sigma-Aldrich, USA) and 1 mg aluminium hydroxide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: Protective antigen (PA) from Bacillus anthracis was cloned into a pET19b vector (Novagen), resulting in a N-terminal His10-tag fusion construct ...
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bioRxiv - Molecular Biology 2020Quote: ... Antigen-antibody complexes were detected with Immobilon ECL Ultra Western HRP substrate (Millipore) on a Chemidoc XRS+ imager (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... Antigen–antibody complexes were detected with Immobilon ECL Ultra Western HRP substrate (Millipore) on a Chemidoc XRS+ imager (BioRad).
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bioRxiv - Bioengineering 2022Quote: ... antigen retrieval was performed with hyaluronidase (10mg/mL in 20mM sodium acetate, Sigma) incubation at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... a short antigen-retrieval with 1x sodium citrate pH 6.0 (Sigma-Aldrich C9999) was performed in a microwave for 5 min at 80% power ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was conducted by incubating in Pepsin Reagent solution (Sigma-Aldrich #R2283) at room temperature for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... the rehydrated sections underwent heat-mediated antigen unmasking with sodium citrate (Sigma-Aldrich) and quenching of endogenous peroxidase activity ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were deparaffinized and rehydrated and underwent antigen retrieval using Trilogy (Sigma-Aldrich) solution and a pressure cooker ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval in 1X Sodium Citrate Buffer (100mM trisodium citrate (Sigma, Cat#S1804), 0.5% Tween-20 (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... sections underwent antigen retrieval in trypsin (Sigma, #, 1g/ml, 10 minutes, 37°C). Sections were permeabilised in 0.25% triton for 30 minutes at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: sections were incubated 30 minutes in 1x antigen retrieval solution (Sigma-Aldrich C9999) at 80°C and washed three times in 1x PBS ...
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bioRxiv - Physiology 2024Quote: ... antigen retrieval was performed following rehydration by incubating with 0.8% hyaluronidase (Sigma-Aldrich) at 37°C for 30 min and antigen retrieval buffer (100 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by boiling samples in citrate buffer (Sigma-Aldrich, C9999) for 30 minutes ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitation of FLAG-Myc was performed by incubating 2 mg of cell lysate with 40 µl of Anti-FLAG M2 affinity gel (Sigma-Aldrich #A2220) for 3h in a final volume of 1 ml of NHEN buffer with agitation at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatants of cell lysates were incubated with magnetic beads coated with anti-Ago 2 or control anti-IgG (Millipore, MA, USA) for 4 h at 4°C ...
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bioRxiv - Genomics 2020Quote: ... was used to determine protein levels of each sample in which 2 mg of nuclear lysates was incubated overnight with 50 uL of ANTI- FLAG M2 magnetic beads (Sigma Cat# M8823) at 4°C or for 2 hours with 20 uL of GFP-Trap magnetic beads (Chromotek ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with polybrene (TR-1003-G ...