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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... and pericytes (neuron-glial antigen 2; NG2; Millipore). tdTomato was used as a reporter of ChR2 expression and amplified with an antibody against mCherry ...
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bioRxiv - Immunology 2020Quote: ... polygyrus antigen or 2 μg/ml concanavalin A (Sigma) at 37 0C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Zoology 2019Quote: ... The zygotes were cultured in potassium-supplemented simplex optimised medium (KSOM, EMD Millipore) at 37°C in 5% CO2 incubator and then injected using a microinjector setup (XenoWorks Micromanipulator and Digital Microinjector by Shutter Instruments) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Neural/Glial antigen 2 (NG2 - Millipore AB5320, 1:100); anti-PDGFRβ (R&D AF385 ...
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bioRxiv - Neuroscience 2022Quote: ... neural/glial antigen 2 (NG2; OPCs; Millipore; AB5320; 1:200), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cell Biology 2021Quote: ... SV40 T Antigen (Ab-2) (Merck Millipore; DP02; 5 μg/ml). Secondary antibodies (all from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... and K-modified simplex optimized medium (KSOM) were purchased from Sigma (St. Louis, MO, USA). Paraformaldehyde (PFA) ...
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bioRxiv - Immunology 2022Quote: ... for the limiting antigen condition and 2 µg/ml (4000 molecules/μm2) for the high antigen condition) and streptavidin (Sigma-Aldrich) were added to the flow chambers with or without 0.5 µg/ml unlabeled histidine-tagged ICAM-1 ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Developmental Biology 2022Quote: ... The fertilized embryos were washed several drops of potassium simplex optimization medium (KSOM; Millipore, MR-106-D) and cultured in the same medium to the blastocyst stage at 37 °C under Ovoil™ (Vitrolife ...
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bioRxiv - Immunology 2023Quote: ... OVA antigen (Sigma) was dissolved in saline to a 1 mg/mL concentration ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were treated with 2 mM 1,10-phenanthroline (Sigma), cross-linked with 0.5 mM DSP (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and NG2 (neuron-glial antigen 2, AB5320, Merck Millipore, Burlington, MA, USA, 1:400) were used.
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed 1X antigen retriever solution (Sigma, pH 6.0). Tissues were incubated with primary antibody in 5% donkey serum ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Developmental Biology 2019Quote: Embryos at all stages were cultured on 35mm Petri dishes (Falcon) within microdrops of 10-15µl of Potassium Simplex Optimized Media with amino acids (KSOM-AA, Millipore), at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... virus-containing media were replaced by selection media containing 2 µg/ml puromycin (Sigma). All assays were performed 7–8 days after infection.
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... All live embryos were cultured in 10μl microdroplets of KSOM (potassium Simplex Optimized Medium; (Lawitts and Biggers, 1991)) with a mineral oil (Sigma, M8410) overlay inside an incubator (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The fertilized eggs were transferred to 5 μL droplets of potassium simplex optimization medium with amino acids (KSOMaa) containing 0.00025% polyvinyl alcohol (P8136-250G; Sigma-Aldrich) and 100 μM ethylenediaminetetraacetic acid on a glass-bottomed dish and placed in an incubation chamber (Tokai Hit ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Unabsorbed virus was removed and 2 ml of culture medium supplemented with 1% methylcellulose (Sigma) were added to each well ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed with pH 6.0 citrate buffer antigen retrieval solution (Sigma #C9999) in a pressure cooker and cooled for 20 minutes at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was filtered through 2-layers of miracloth (Millipore, Cat. 475855) set up in a 50[ml centrifuge tube ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen Retriever (Sigma, Cat. No. C9999). The Vectastain Elite ABC Universal kit peroxidase (Horse Anti-Mouse/Rabbit IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... C4-2 cell lysates were incubated with antibodies for AR (Sigma, Cat. No.A9853), YAP (Santa Cruz ...
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bioRxiv - Cell Biology 2023Quote: ... samples were incubated with benzonase (Sigma Aldrich, 2 μl/500 μl of lysate) for 90 minutes at 4°C on a rotating wheel ...
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bioRxiv - Neuroscience 2023Quote: ... Sections underwent antigen retrieval using 1x Citrate buffer pH6.0 antigen retriever solution (C9999, Millipore-Sigma, Burlington, MA), at 60°C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Sections underwent antigen retrieval using 1x Citrate buffer pH6.0 antigen retriever solution (C9999, Millipore-Sigma, Burlington, MA), at 60°C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... were cultured in 24 well plates and stimulated with inactivated SARS-COV-2 antigen (1.2 μg/ml) or PMA (25 ng/ml, cat # P8139; Sigma) and Ionomycin (1 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... The antigen-retrieval buffer used for live-cell lysis was 2× Tris-glycine buffer (Sigma Aldrich, St. Louis, MO) supplemented with 1% SDS and 12 M urea (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Genomics 2019Quote: ... The lysate was then mixed with 2 ml of 50% Iodixanol (Sigma-Aldrich D1556) to generate a nuclei suspension with 20% Iodixanol ...
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bioRxiv - Developmental Biology 2022Quote: ... Muscle lysates were further digested with 2 IU/ml of Dispase II (Sigma Aldrich) diluted in minimum amount of PBS for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... remaining lysate was equally divided and incubated with 2 µg Pan-Ago (Millipore; 2A8) or IgG (Cell Signaling 5415 ...
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bioRxiv - Genomics 2023Quote: ... The lysate was then mixed with 2 mL of 50% Iodixanol (Sigma-Aldrich D1556) to generate a nuclei suspension with 20% Iodixanol ...