Labshake search
Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Hepatitis E Virus Capsid Protein ORF2 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The capsid binding inhibitor PF74 was purchased from Sigma-Aldrich (CAT# SML0835). The HIV-1 protease inhibitor Saquinavir (ARP-4658 ...
-
bioRxiv - Microbiology 2021Quote: ... Mouse anti-histidine tag 1:200× (Sigma-Aldrich, St. Louis, MO) and HRP-conjugated goat anti-mouse IgG 1:1,000× (Seracare Life Sci. ...
-
bioRxiv - Neuroscience 2023Quote: ... and a mouse monoclonal anti-FLAG epitope-tag antibody (Sigma #F7425). For the detection of endogenous Pdlim5 ...
-
bioRxiv - Cell Biology 2020Quote: ... CDC20 (mouse mAb clone E-7, Santa Cruz sc-13162; mouse mAB, clone AR12, Millipore UK Ltd ...
-
bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
-
bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
-
bioRxiv - Genetics 2021Quote: ... Proteins were then transfered to an Immobilon-E PVDF membrane (Millipore), which was then washed with 1× PBS containing 0.05% Tween 20 (three washes ...
-
bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
-
bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
-
bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
-
bioRxiv - Microbiology 2024Quote: ... Capsids were then opened by addition of 50 μg/ml proteinase K (Sigma) and 1% SDS to each sample ...
-
bioRxiv - Immunology 2023Quote: ... Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich #A2429), at a 1:4000 dilution was added ...
-
bioRxiv - Biochemistry 2019Quote: ... rabbit monoclonal DYKDDDDK tag (Flag tag, 1:1000, CST, #14793) and/or mouse monoclonal α-tubulin (1:10,000, Millipore, 05-829). Following three washes with TBS-T ...
-
bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase (HRP)-conjugated mouse anti-FLAG tag antibody (A8592, Sigma-Aldrich) was used to detect VH binding ...
-
bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
-
bioRxiv - Immunology 2022Quote: ... Commercially available flavivirus E protein-specific mAb 4G2 (EMD Millipore, Darmstadt, Germany), rabbit anti-ZIKV prM and C antibodies (GeneTex ...
-
bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
-
bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
-
bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
-
bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
-
bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
-
bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
-
bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
-
bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-FLAG-tag (1:5,000, mouse mAb, F1804, Sigma-Aldrich, St. Louis, MO), anti-alpha-tubulin (1:2,000 ...
-
bioRxiv - Microbiology 2023Quote: Antibodies were as follows: commercial mouse monoclonal antibodies to Flag-tag (M2; Sigma), Strep-tag (4F1 ...
-
bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...
-
bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
-
bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
-
bioRxiv - Microbiology 2022Quote: ... The full capsid region was then amplified from the cDNA using KOD polymerase Mastermix (Novagen) and the primers CVB3_P1_seq_F (CCC TTT GTT GGG TTT ATA CCA CTT AG ...
-
bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: ... coli BL21(DE3) as a fusion protein containing an N- terminal His6-tag (Novagen) and purified by metal-affinity chromatography (Ni-NTA ...
-
bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
-
bioRxiv - Plant Biology 2022Quote: ... and anti-Mouse IgG (Fc-specific)-Peroxydase (Sigma, dilution: 1/10000, 1.5hr incubation). Anti-Hsp90 (Agrisera ...
-
bioRxiv - Microbiology 2023Quote: ... Biotinylated goat anti-mouse IgG (diluted 1:500 in 10% FCS) (Sigma-Aldrich) was added to the wells and incubated for 1 h at 37°C ...
-
bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
-
bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
-
bioRxiv - Microbiology 2023Quote: ... strep tag and myc tag were obtained from Sigma, IBA and Abcam ...
-
bioRxiv - Microbiology 2022Quote: ... Mouse monoclonal antibody MAB8150 against WNV E glycoprotein was from EMD Millipore (Billenca MA). Alexa Fluor 488 goat anti-mouse IgG (H+L ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
-
bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
-
bioRxiv - Microbiology 2024Quote: ... Fixed cells were first incubated with anti-EV-A71 capsid monoclonal antibody MAB979 (1:1000; Sigma) for 1 hour at 37 °C and then with Alexa Fluor 488-conjugated secondary antibodies (1:2000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich) were used.
-
bioRxiv - Molecular Biology 2020Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma Aldrich) were used.
-
bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...
-
bioRxiv - Plant Biology 2023Quote: ... Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma) at the 1:1000 dilution ...
-
bioRxiv - Immunology 2023Quote: ... 68] (dilution 1:1000) and mouse mAb to FLAG tag (Sigma, F3165, dilution 1:500).
-
bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
-
bioRxiv - Cancer Biology 2020Quote: Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene ...