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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Hepatitis E Virus Capsid Protein ORF2 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Microbiology 2023Quote: ... immunostained with an ORF2 antibody (1E6 1:1000, Millipore) at 4 °C overnight followed by anti-mouse Alexa-594 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... and/or 2.5 μg/ml E-cadherin-Fc (Sigma). IELs were plated in RPMI-T medium containing 2 μg/ml PerCP-e710 conjugated anti-CD107a (1D4B ...
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bioRxiv - Microbiology 2021Quote: ... MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I, Merck Millipore, France).
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bioRxiv - Cell Biology 2019Quote: ... ORF2-Flag were detected using FLAG-M2 antibody (Sigma, cat. number F1804) at 1:1000 dilution.
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-ORF2 mAbs at 1:1000; mouse anti-Flag M2 (Sigma F1804) at 1:2000) ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Molecular Biology 2019Quote: ... Flag-tag [(anti-mouse: Sigma, F1804)(anti-rabbit ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-S-Tag (EMD Millipore); Mouse anti-GAPDH (Santa Cruz Biotechnology) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... bound ACE2-mouse Fc was detected by HRP-conjugated anti mouse IgG (Fc specific) (Sigma-Aldrich). Another way to do hACE2 competition ELISA is to incubate the fixed concentration of biotinylated hACE2-Fc with gradient concentrations of antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-Fc (M4280, Sigma Aldrich) 1:100 ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Cell Biology 2023Quote: ... HA tag (mouse monoclonal; Sigma-Aldrich, H3663), HA tag (rabbit polyclonal ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse Flag-tag (1:5000, Millipore-Sigma), rabbit Flag-tag (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse Flag-tag (1:5000, Millipore-Sigma), rabbit Flag-tag (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: Anti-Japanese encephalitis E (mouse monoclonal, Millipore), influenza A H9N2 HA (mouse monoclonal ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed and processed for indirect immunofluorescence with anti-ORF2 antibody 1E6 (Millipore). For confocal microscopy analysis ...
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bioRxiv - Neuroscience 2023Quote: ... CopiaGag capsid protein was concentrated using a 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit(EMD Millipore) to a final concentration of 0.8 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-labeled goat anti-mouse Fc (Sigma, A0168 ...
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bioRxiv - Molecular Biology 2019Quote: ... Myc-tag [(anti-mouse: EMD Millipore, 05-724)(anti-rabbit ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GST tag (G1160, Sigma 1:2000), mouse anti-His tag (HIS.H8 / EH158 ...
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bioRxiv - Physiology 2020Quote: ... mouse anti-S-tag (1:1500, Sigma, SAB2702204); rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-FLAG-tag (M2, Sigma-Aldrich, #F1804), rabbit anti-GFP-tag (Thermo-Fisher ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-Flag tag (F1804, Sigma-Aldrich, USA); rabbit anti-BGLAP (A6205 ...
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bioRxiv - Cancer Biology 2024Quote: ... Flag-tag M2 mouse antibody (Sigma-Aldrich, F1804), Ub (P4D1 ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the capsid protein were pooled and concentrated using an Amicon Ultra-15 centrifugal filter (Merck Millipore), and the buffer was exchanged to a final storage buffer composed of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Neuroscience 2023Quote: ... A260/A280 = 0.6) capsid protein was concentrated using a 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit (EMD Millipore) to a final concentration of 1.4 mg/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Neuroscience 2019Quote: ... anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich), anti-tubulin # T8660 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated mouse anti-FLAG tag Ab (Sigma-Aldrich) was used to detect Fab/VH binding ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal Flag-tag antibody (Sigma, F3165, 1:1000), rabbit monoclonal HA-tag antibody (CST ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-Flag tag (Sigma cat# F1804, 1:1000), rabbit anti-human actin (Sigma cat#A2066 ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal HA-Tag and Mouse Monoclonal Flag antibodies purchased from Sigma (USA); Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Immunology 2021Quote: ... or anti-mouse IgG (Fc-specific)-HRP (Sigma, A2554) secondary antibody respectively ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-mouse IgG (Fc) TRITC (Sigma #SAB3701020, 1:500), Anti-rabbit IgG FITC (Sigma #F7512 ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-FLAG tag (clone M2-F1804, Sigma, 1:1000), mouse anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-HA-Tag (1:500, rabbit Sigma-Aldrich H3663), anti-FLAG (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with mouse anti-E-cadherin (Millipore, MAB1199) at 1:200 dilution in blocking solution at 37°C for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Immunology 2023Quote: ... and stained as described previously [34] using an anti-ORF2 monoclonal antibody (1:400, 1E6; Millipore, Burlington, MA) and a secondary anti-mouse antibody conjugated to Alexa Fluor 594 (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...