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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The protein-antibody complex was visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-Sepharose (P3391) and PRMT5:MEP50 active complex (SRP0145) were purchased from Sigma Aldrich. Protein co-immunoprecipitation was performed using ANTI-FLAG® M2 Affinity Gel (A2220 ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant proteins (PRMT5:MEP50 complex (SRP0145-25470) and EZH2 (SRP0379) were purchased from Sigma-Aldrich USA ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Biochemistry 2022Quote: ... complex samples were diluted 1:1 (v/v) with d8-glycerol (Sigma Aldrich) and 30 µL sample solution was filled into 3 mm quartz tubes ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Neuroscience 2021Quote: ... corticosterone:HBC complex (Sigma-Aldrich) was dissolved in saline and administered at 2mg/Kg IP ...
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bioRxiv - Immunology 2020Quote: ... Protein production was done in HEK293-6E cells by transient transfection with polyethylenimine (PEI, Sigma-Aldrich) and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mL/disc of 10 wt% cetylpyridinium chloride (Sigma) was added to the discs ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 wt% (3-Glycidyloxypropyl)trimethoxylane (440167, Sigma-Aldrich) before curing overnight in an oven at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% wt/vol of 400 cP methylcellulose (Sigma). Mammosphere media was prepared and placed on a rocker overnight at 4’C to dissolve methylcellulose ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% (wt/wt) N,N’-methylenbisacrylamide (Sigma, cat.no. M1533) in 1X PBS) ...
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bioRxiv - Bioengineering 2019Quote: ... 30% (wt/wt) solution (Sigma-Aldrich, cat. no. A3699), sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitated antibody/chromatin complex was collected using Protein A/G magnetic beads (Millipore 16-663). The input and ChIP samples were reverse cross-linked overnight at 45°C using 0.2M final concentration of NaCl ...
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bioRxiv - Immunology 2019Quote: ... CD14 peridinin-chlorophyll protein complex (PerCP, clone TM1, DRFZ Berlin) and propidium iodide (PI, Sigma Aldrich).
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Cell Biology 2019Quote: ... the Permanox surface was covered with 2 μg/ml laminin (catalog no. L2020-1MG, Sigma, and catalog no. LNKT-0201, Biolamina) in DMEM (200 μl/well) ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Cancer Biology 2022Quote: ... PBS and 1 μg/mL Human Plasma Fibronectin Purified Protein (Sigma-Aldrich, Cat. #: FC010). 1N NaOH was added to achieve a neutral ∼pH and a thin base layer of 100 μL volume was added to the well of a 48-well tissue culture plate precoated with 1% agarose ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Membrane attack complex (MAC) binding was assessed by staining with rabbit anti-human C5b-C9 antibody (Catalog #204903, Sigma) at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903 ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were treated as previously reported to purify the target proteins by immunoaffinity chromatography using anti-HLA-DR-FF-sepharose or M2 anti flag (Sigma)(Álvaro-Benito et al. ...
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bioRxiv - Cell Biology 2023Quote: WT Ub (Sigma) and non-cleavable Ub mutant (Ubnc ...
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bioRxiv - Cell Biology 2020Quote: ... RPE-1 WT cells were treated with nocodazole (Sigma-Aldrich) at (5 μM ...
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bioRxiv - Microbiology 2022Quote: ... Cells then were overlaid with 1% (wt/vol) methylcellulose (Sigma) in Opti-MEM supplemented (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Filipin complex (Sigma-Aldrich, F9765), Nigericin sodium salt (Cayman Chemical ...
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bioRxiv - Immunology 2022Quote: ... Antibody production was conducted by co-transfection of the heavy and light chain expression vectors into Human HEK293 suspension culture cells (Expi293F) using polyethylene mine (Sigma). Cells were subsequently cultured for 4 days before being pelleted by centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Microbiology 2019Quote: WT ppr-SiiA protein and mutant variants were produced using freshly transformed BL21(DE3) (Novagen) cells ...
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bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
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bioRxiv - Biochemistry 2022Quote: WT and mutant Streptococcus pyogenes Cas9 proteins were expressed in E.coli Rosetta 2 (DE3) (Novagen) for 16 hours at 18 °C as fusion proteins with an N-terminal His6-MBP-TEV tag ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody-bound chromatin complex was precipitated with salmon sperm DNA/Protein A agarose (EMD Millipore, Burlington, MA), then washed sequentially with PIRA (150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Protein complex was incubated overnight on ice and then concentrated onto 30 kDa MWCO Amicon concentrator (Millipore) until protein concentration reached 11 mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Immunodetection of ProteinA (ProtA)-tagged proteins was performed using Peroxidase Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich) in a 1:3,000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... The ORF1p-FLAG protein complex bound was eluted using 200 μg/mL of 3xFLAG peptide (Sigma-Aldrich) in the Lysis150 buffer containing 0.2 mM PMSF and 1x cOmplete EDTA-free protease inhibitor cocktail by incubation at 4°C for 1 hour with gentle rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...