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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... anti-complex I – 75 kDa subunit (ABN302, EMD Millipore), anti-complex III ubiquinol-cytochrome C reductase core protein 2 (UQCRC2 ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T and HEK293-FT cells were maintained in DMEM supplemented with 10% FBS,1 mM sodium pyruvate (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 40 μL of CTEP was mixed with vehicle [1 wt% Hypromellose (HPMC) (Sigma catalog #H3785) and 1 wt% Tween-80] in an IKA Ball-Mill Tube BMT-20-S containing 10 stainless steel balls as described above to a final concentration of 0.2 mg/mL CTEP ...
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bioRxiv - Biophysics 2023Quote: ... The wt cells were additionally supplemented with 10 ng ml−1 EGF (E9644, Sigma-Aldrich), while mutant cell liens were maintained without EGF ...
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bioRxiv - Microbiology 2023Quote: ... then washed three times in DBPS containing 1% (wt/vol) bovine serum albumin (BSA; Sigma). Bleached oocysts were stored for up to 1 week in DBPS plus 1% BSA at 4 °C before infection ...
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bioRxiv - Bioengineering 2023Quote: ... using a solution of 0.03 wt % lithium phenyl-2,4,6-trimethylbenzoylphosphinate (1 mM; LAP, Sigma-Aldrich), 0.1 mg/mL of peptide and 1 mg/mL of BSA ...
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bioRxiv - Cell Biology 2024Quote: MEFDMs were generated by seeding WT MEFs (on 1% gelatin coated dishes (Porcine gelatin, Sigma) at a density of 4.6 x 105/cm2 as described elsewhere in 36.
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Genetics 2023Quote: ... at 37 °C on a heating plate with 10µL digestion mix (3% [wt/vol] pectolyase Y-23 [MPBiomedicals], 0.3% [wt/vol] Driselase [Sigma] 0.3% [wt/vol] cellulase [OnozukaR10] [Duchefa] 0.1% sodium azide in 10 mM citrate buffer) ...
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bioRxiv - Cell Biology 2020Quote: Wild type Hek293 cells were grown in DMEM (Sigma D6429) supplemented with 10% FCS ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... while HEK293 and A549 cells were cultured in DMEM (Sigma). Both were supplemented with 10% foetal calf serum (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: Hela and HEK293 cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10 % fetal bovine serum (PAN Biotech) ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were cultured in high glucose DMEM (Sigma-Aldrich) containing 10% FBS (HyClone) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cell lines were cultured with MEM medium (Sigma-Aldrich) supplied with FBS (Funakoshi) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% fetal bovine serum ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were incubated in culture with 1.5% DMS (Sigma) at room temperature for 7 minutes ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured on poly-D-lysine (Sigma-Aldrich) coated glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: HEK293 derived-nsEVs were resuspended with dextran-FITC (Sigma-Aldrich) or seed saporin (SAP ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 T-REx cells were treated with RocA (Sigma– Aldrich) at a concentration of 0.3 μM or 3 μM and incubated for 30 min before cell lysis ...
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bioRxiv - Cancer Biology 2020Quote: HEK293-T cells were transiently transfected with GFP-V5 or GREP1-V5 fusion proteins using OptiMem and Fugene HD (Sigma-Aldrich, St. Louis, MO). 72 hours after transfection ...
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bioRxiv - Molecular Biology 2023Quote: The dox-inducible A375P cells were treated with doxycycline to express MITF WT and MITF mutant proteins for 24 hours and then treated with 40 µg/ml cycloheximide (Sigma #66819), in presence or absence of 200nM TPA ...
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bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... A horseradish peroxidase-labeled anti-His tag antibody (1:25000 dilution; Sigma-Aldrich, Poland) diluted in 5% skimmed milk / TBS-Tween (0.1% ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Bioengineering 2020Quote: ... 10 wt% triethanolamine (Sigma Aldrich, 90278-100mL) with DI water at 55°C until also fully dissolved ...
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bioRxiv - Bioengineering 2020Quote: ... 60 wt % dispersion in H2O (Sigma-Aldrich). The syringe was clamped into a polymer blender and mixed gently for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 11 wt% 2,2’,2”,-nitrilotriethanol (V900257, Sigma), and 16 wt% distilled water ...
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bioRxiv - Microbiology 2021Quote: ... Taurocholate (TA, 0.1% wt/vol, Millipore Sigma), thiamphenicol (TAP ...
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bioRxiv - Cell Biology 2020Quote: ... and 10% (wt/vol) dextran sulfate (Sigma). Samples were incubated in a humid chamber inside a hybridization oven at 37 °C for 40 h ...
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bioRxiv - Genetics 2021Quote: ... 0.05% (wt/vol) saponin (Sigma-Aldrich, Germany), and phosphate-buffered saline at 4°C for 3 days ...
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bioRxiv - Immunology 2022Quote: ... and dexamethasone 1mM/mL (wt/vol, Sigma), for differentiating macrophages ...
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bioRxiv - Cell Biology 2023Quote: ... 10% wt/vol glucose (Sigma-Aldrich, G5767), 2 mM Trolox (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.1 wt% Tween-20 (Sigma Aldrich) in PBS ...
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bioRxiv - Pathology 2023Quote: ... 2% (wt/v) sodium deoxycholate (Sigma Aldrich), 1 M NaCl (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... 40 wt% of acrylamide (AAm, Sigma-Aldrich) and 0.2 wt% of N,N′-methylenebis(acrylamide ...
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bioRxiv - Bioengineering 2023Quote: ... 0.5 wt% Pluronic F-127 (Sigma-Aldrich), and 0.2 wt% gum arabic (Alfa Aesar ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% (wt/vol) dextran sulfate (Sigma, #D8906), 1 μg/μL yeast tRNA (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... and input TRF1 mutants were revealed with anti-His (anti 6-His Rabbit Pab Sigma Aldrich). For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Microbiology 2020Quote: Female BALB/c mice have been injected intraperitoneally with 700 μg recombinant His-tagged TGT protein that was emulsified in complete Freund’s adjuvant (Sigma-Aldrich). Two weeks later ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...
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bioRxiv - Microbiology 2022Quote: ... The polyclonal anti-Hcp antibody was developed after immunization of six-week-old female BALB/c mice with three subcutaneous injections of the purified His-Hcp protein emulsified in Freund adjuvant (Sigma).
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bioRxiv - Biochemistry 2023Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...