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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Estrone 3 Glucuronide E1G ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: HMGB1 levels were measured using a commercial kit (HMGB1 ELISA kit A76696, Sigma-Aldrich). The assay was performed as described in the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: ... DNA probes (5’-GTTATGAGCCCGACGAGCTACCAGGCTGCT-3’) with a 5’-ethylcarbamate amino linker (Sigma-Aldrich) were covalently immobilized on NHS-activated Sepharose 4 Fast Flow (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... The plate was blocked with 5% BSA (Sigma) in PBS for 3 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Biophysics 2021Quote: ... was performed using a pre-annealed DNA-RNA template (DNA45: 5’-TTCTTT ATCTCTTATTTCCTTCTATTTTCCACGCGCCTTCTATTT-3’; RNA13: 5’-[6FAM]-GGCGCGUGGAAAA-3’, Sigma Aldrich). The reaction buffer comprised 25 mM HEPES pH 7.2 ...
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bioRxiv - Neuroscience 2019Quote: ... KO2 along with Cas9 donor plasmid were used as template DNA and amplified with F 5’-GAAGAAGAATGGCCTGTTCG-3’ and R 5’-GCCTTATCCAGTTCGCTCAG-3’ with KOD Xtreme (Novagen, #71975). Cycling conditions were as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... 53 from the RNAi Consortium (clones TRCN0000087290 and TRCN0000087292 with target sequence 5’-GCGGGAGTATAGTGAGTTTAA-3’ and 5’-CGGACAGTTTGGCACAATCAA-3’, respectively, distributed by Sigma-Aldrich, Merck ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... Equal concentrations of serially diluted lysate (in the range of 0-60 μg/ml) from control and Thap1 cKO genotypes was incubated with 10 mM 4-methylumbelliferyl β-D-glucuronide (MUG, Sigma) in 0.2M Sodium acetate buffer ...
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bioRxiv - Microbiology 2019Quote: We performed ex vivo tissue imaging of intestinal bacterial GUS activity through quantification of fluorescence following hydrolysis of fluorescein di-b-D-glucuronide (FDGlcU, Sigma-Aldrich) to fluorescein (65) ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Physiology 2020Quote: ... and GLP-1 ELISA kit Active (EGLP-35K, Millipore, MA, USA), respectively.
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bioRxiv - Physiology 2021Quote: ... and IL-6 (KIT ELISA IL-6-RAB0308-1KT, Sigma-Aldrich) in BALF following the kit instructions ...
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bioRxiv - Neuroscience 2023Quote: The S100B ELISA Kit (#EZHS100B-33K; EMD Millipore, Billerica, MA, US) and Human MMP-14 ELISA Kit (#ab197747 ...
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bioRxiv - Microbiology 2023Quote: ... was frozen for ELISA analysis (IL-1β kit (Sigma-Aldrich RAB1108). MMP9 ELISA ...
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bioRxiv - Immunology 2022Quote: ... Nunc 96-well ELISA plates were coated with 50 µl of 2 µg/ml SA (Sigma) in borate saline buffer (100 mM boric acid ...
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bioRxiv - Immunology 2023Quote: Plates were coated with 3 μg/ml human IgM (Millipore) in 0.1 M sodiumcarbonate ...
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bioRxiv - Biochemistry 2023Quote: ... diluted 1:10,000 in ELISA buffer (PBS, 5% Tween20, 1% goat serum (Sigma-Aldrich, G9023)) was added to all wells ...
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bioRxiv - Microbiology 2022Quote: ... Both strains were initially grown anaerobically at 37°C for 3–5 days on Bifido Selective Media (BSM) agar plates (Millipore Sigma, Burlington, MA), from which a single colony was selected and grown in BSM broth (Millipore Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... Knock-down of glutamate dehydrogenase 2 (GDH2, encoded by the GLUD2 gene) using siRNAs (#NM_012084: 5’CUAACCUCUUCACGUGUAA’3 and 5’UUACACGUGAAGAGGUUAG‘3, Sigma-Aldrich, Taufkirchen, Germany) or transfection of GLUD2 plasmid/empty vector controls was done using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary guide oligonucleotides (forward: 5’-CACCGCAGGATTGAAGACCTTAACA-3’; reverse: 5’-AAACTGTTAAGCTCTTCAATCCTGC-3’) were custom synthesized separately by Sigma-Aldrich (St. Louis, MO, USA), annealed ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μM carbonyl cyanide 3-chlorophenylhydrazone (Sigma Cat # C2759) and 5 μM valinomycin (Sigma Cat # V0627) ...
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bioRxiv - Genomics 2021Quote: ... a 3-day puromycin (5 μg/mL, Sigma, P8833) selection was performed 48 hours after the spin-inoculation ...
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bioRxiv - Microbiology 2021Quote: ... siDDX42-2: 5’-CUUACCUUGUGUUUGAUGA-3’ (SASI_Hs01_00119845, Sigma-Aldrich®), siDDX42-4 ...
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bioRxiv - Biophysics 2020Quote: ... mglBΔCT-R (5’-CGTAAAGCTTTTACACCAGGCTCTCGAAGATCTTCGTGAGCTC-3’ synthesized by Sigma-Aldrich, India ...
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bioRxiv - Cell Biology 2019Quote: ... siRNA Piezo1 (Sigma-Aldrich: 5’-GCAAGUUCGUGCGCGGAUU[dT][dT]-3’), ON-TARGET plus SMARTpool human siRNA ADAM10 (Dharmacon) ...
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bioRxiv - Neuroscience 2021Quote: ... or non-targeting siLUC (5’-UAAGGCUAUGAAGAGAUAC-3’, Sigma-Aldrich) were mixed with 54 μl of RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Forward siRNA sequences 5’-CAGUGGCAGUGGACAAUUCA[dT][dT]-3’ (Sigma) and 5’-UCGUGGAAAGUUUGCUGCAGGGAAA[dT][dT]-3’ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... we used the siRNA duplex 5′-UUCUCCGAACGUGUCACGUdTdT-3′ (Sigma). The cells were further processed according to the experimental design and depletion was assessed by immunofluorescence ...
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bioRxiv - Cell Biology 2023Quote: ... TDP2 (5’ GUGGUGCAGUUCAAGAUCAtt 3’; obtained from Sigma-Aldrich/Merck) and non-targeting siRNA (Silencer® Select Negative Control No ...
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bioRxiv - Cell Biology 2022Quote: ... 50 nM Rab14 siRNA (5′-CAACUACUCUUACAUCUUU-3′, Sigma-Aldrich) for 72 h using Lipofectamine 2000 ...
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bioRxiv - Genetics 2023Quote: ... scrambled (NT) shRNA (5’-GCGATAGCGCTAATAATTT-3’ SHC202; Sigma-Aldrich) or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence ...
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bioRxiv - Genetics 2024Quote: ... moxidectin (3 nM) (Millipore Sigma, Catalog # 113507-06-5), ricobendazole (25 μM ...
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bioRxiv - Neuroscience 2021Quote: ... All ELISAs were developed using ELISA TMB (Sigma-Aldrich) and absorbance read on a Bio-Tek plate reader ...
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bioRxiv - Biochemistry 2021Quote: ... and GUS activity was measured after 30 minutes of incubation in the presence of 1 mM 4-methylumbelliferyl-β-D-glucuronide (MUG, Sigma Aldrich). Reactions were stopped by the addition of four volumes of 0.2 M Na2CO3 and fluorescence was measured in a Biotek Synergy microplate reader with excitation and emission wavelengths of 365 nm and 455 nm ...
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bioRxiv - Plant Biology 2022Quote: ... 5 μL of cell lysate was added to 45 μL of 10 mM 4-methylumbelliferyl-β-D-glucuronide solution (MUG, M-9130; Sigma-Aldrich). The reaction was stopped after 1 hour incubation at 37°C by adding 220 μL of 0.2 M Na2CO3 ...
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bioRxiv - Physiology 2019Quote: ... transgenic soybean hairy roots and tobacco leaves were used to determine GUS enzyme activity by measuring the fluorescence of 4-methylumbelliferone (4-MU) produced by GUS cleavage of 4-methylumbelliferyl-β-d-glucuronide (4-MUG, Sigma, USA) according to the published procedure as described previously (Jefferson ...
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bioRxiv - Cell Biology 2020Quote: ... The plate was washed with 5% Hellmanex Ⅲ (Sigma) overnight and 5 M NaOH for three times ...
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bioRxiv - Cell Biology 2022Quote: 96-well ELISA plates were coated overnight at 4°C with anti-mouse total β1-integrin (Millipore) or anti-mouse β1-integrin ...
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were washed five times with wash buffer (1 X PBS/0.05% Tween 20 (Sigma)) and blocked with 100 µl/well 5% FBS (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Physiology 2021Quote: ... Plasma FGF21 was assayed using the rat/mouse FGF21 ELISA kit (Sigma) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-90K, Millipore). All procedures were performed by following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... commercially available ELISA kits were used to measure lactose content (Sigma-Aldrich Cat ...
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bioRxiv - Immunology 2021Quote: ... which was analyzed by enzyme linked immunosorbent assay (ELISA) kit (Sigma, MO).
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bioRxiv - Developmental Biology 2022Quote: ELISA kits were used for measurements Growth Hormone (Merck-SIGMA EZRMGH-45K) according to manufacturers’ instructions.
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bioRxiv - Neuroscience 2022Quote: ... S100B ELISA kits were purchased from Millipore (EMD Millipore, St. Louis, MO) and samples were diluted 1:1 ...
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bioRxiv - Neuroscience 2023Quote: ... Plasma was processed using an insulin ELISA kit (Millipore Sigma; #EZRMI-13K) following manufacturer’s instructions in duplicate ...