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Citations for Millipore Sigma :
201 - 250 of 886 citations for Ebola Virus Like Particles since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was backfilled into a pipette of mineral oil (M5904, Sigma-Aldrich). We injected 400nl virus into the identified C2 barrel column through a single injection site over 4 min at 300μm below pia ...
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bioRxiv - Immunology 2020Quote: Inactivated virus was concentrated using Amicon Ultra Centrifugal Filter units (Millipore Sigma) at 100k cutoff ...
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bioRxiv - Microbiology 2020Quote: ... Unabsorbed virus were removed and cells were overlaid with MEM (Sigma-Aldrich) containing 0.65% agarose (Nacalai tesque ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-infected GL261 cells were trypsinized with 0.25% trypsin (Sigma-Aldrich) 72 hours after infection ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cancer Biology 2023Quote: ... An empty TRC1 lentiviral construct was used as the Control virus (Sigma).
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bioRxiv - Cancer Biology 2023Quote: ... The virus was concentrated using Amicon Ultra filters (Millipore cat. no. UFC910024) until they have about 1.5ml of concentrated virus ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore, TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with virus along with 10 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded at a density of 1 × 106 cells per well in 12-well plates and stimulated with LPS (100 ng/mL, M1-like - Sigma-Aldrich), IL4 (10 ng/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Neuroscience 2020Quote: ... SH-SY5Y cells were differentiated to a neurone-like phenotype prior to experimentation by incubation with 10 μM trans-retinoic acid (Sigma, UK) for 5 days [26].
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bioRxiv - Neuroscience 2021Quote: ... Three wells of BMEC-like cells per condition were then lysed in PBS containing 5% Triton X-100 (Sigma-Aldrich #T8787), and one well per condition was fixed in ice cold 100% methanol ...
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bioRxiv - Microbiology 2020Quote: For the measurement of proteasome activity a fluorometric assay kit (Proteasome 20S Activity Assay kit) that measures the chymotrypsin-like protease activity was used following manufactured instructions (Sigma-Aldrich). A total of 100 µg of protein extracts were incubated with LLVY-R110 substrate provided by the kit for 2 h at 30°C in dark ...
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bioRxiv - Microbiology 2020Quote: ... Macrophage-like THP1 cells were pre-incubated with L-glutamic acid (5mM) or Sodium Pyruvate solution (2mM, Sigma catalog# S8636-100ml) for 2 hours before stimulating with Escherichia coli serotype O127:B8 LPS (50ng/ml ...
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bioRxiv - Microbiology 2019Quote: Macrophage-like DH82 cells (ATCC CRL-10389) were grown in 25cm2 flasks with Eagle’s Minimum Essential Medium (EMEM) (Sigma, M0325, USA) with 10% heat inactivated FBS at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... THP-1 cells differentiation into mature macrophages-like state was induced through incubation with 10 ng/mL of phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 48 hours in a humidified atmosphere ...
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bioRxiv - Physiology 2021Quote: A drop of fresh culture containing control or spaghetti-like bacteria with 2 μM DiI dye (Sigma, CAS #41085-99-8) was placed on an NGM agar plate ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lysates and concentrated particles were analyzed for FLAG (Sigma–Aldrich; A8592; 1:10,000), GAPDH (Sigma–Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... were used to generate lentivirus particles expressing PP4C-targeting shRNA obtained from Sigma: shRNA-1 (TRCN0000010737) ...
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bioRxiv - Cancer Biology 2019Quote: ... Medium containing lentiviral particles and 8 mg/mL polybrene (Sigma-Aldrich, St. Louis) was used to infect NOKs or HTK16 cells ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with lentiviral particles and 8 µg/ml polybrene (Sigma-Aldrich) for 1 hour upon spinfection and selected for several days using puromycin (InvivoGen) ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were transfected with lentiviruses particles containing Pin1 shRNA (Sigma-Aldrich, TRCN0000010577) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: A ZIC-pHILIC 150 × 2.1 mm (5 µm particle size) column (EMD Millipore) was employed on a Vanquish Horizon UHPLC system for compound separation at 40 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Rictor-/- MEFs were infected with lentiviral particles encoding an shRNA targeting TBK1 (Sigma) (mouse TBK1 # TRCN0000323444 ...
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bioRxiv - Microbiology 2021Quote: ... Medium containing lentiviral particles and 8 mg/mL polybrene (Sigma-Aldrich, St. Louis) was used to infect Akata (EBV+ ...
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bioRxiv - Biochemistry 2020Quote: ... Lentiviral particles were propagated in HEK293T cells using the polyethylenimine (Sigma, Cat# 408727) cell transfection method as described previously (Purroy et al. ...
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bioRxiv - Cancer Biology 2021Quote: A ZIC-pHILIC 150 × 2.1 mm (5 µm particle size) column (EMD Millipore) was employed on a Vanquish Horizon UHPLC system for compound separation at 40°C ...
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bioRxiv - Molecular Biology 2023Quote: ... AAV particles from the supernatant were precipitated by adding polyethylene glycol 8000 (Sigma) to a final concentration of 8% (w/v) ...
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bioRxiv - Genetics 2022Quote: Viral particles were made from shRNA lentiviral vector targeting human EGR1 (Sigma-Aldrich MISSION shRNA Target Clone ID TRCN000027385 ...
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bioRxiv - Cell Biology 2022Quote: ... Mission shRNA Lentiviral particles designed to target CD95L were purchased: shL3 (Sigma TRCN0000059000), shL1 (Sigma TRCN0000058999) ...
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bioRxiv - Plant Biology 2023Quote: ... Quartz sand (50-70 mesh particle size, non-acid washed, Sigma-Aldrich, USA) was used for all other experiments ...
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bioRxiv - Physiology 2023Quote: ... To facilitate viral particle entry in the cells 2ug/mL polybrene (Sigma Aldrich) we added to the culture media ...
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bioRxiv - Microbiology 2024Quote: Viral particles from CPE-positive cultures were recovered through 0.22µm filters (Millipore, Merck). From an aliquot of 140 μl of the supernatant ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... Virus was thawed at 37°C and 5μl 10mg/ml polybrene (Sigma H9268) was added to the virus ...
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bioRxiv - Biochemistry 2021Quote: ... Cholesteryl hemisuccinate (CHS) and tobacco etch virus (TEV) protease were from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... virus containing supernatant was harvested and filtered (0.45 μm, Millex-HV Filter; Millipore). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were infected with virus in the presence of 8μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Live filtered virus was used to transduce cells with polybrene (10μg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... the virus was concentrated using Amicon ultra-15 centrifugal filter units (Millipore Sigma) by centrifuging at 3000 rpm and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Virus-containing supernatants were filtered through a 0.45-μm-pore-size filter (Millipore) and supplemented with polybrene (8 μg/ ml ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).