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Citations for Millipore Sigma :
601 - 650 of 878 citations for Ebola Virus Envelope Glycoprotein GP Zaire Kikwit 95 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: mESCs were grown at 37 °C under a 95% air and 5% CO2 atmosphere on gelatin in serum/LIF medium composed of GMEM (Sigma, G5154) supplemented with 15% FBS (HyClone™) ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Cells were washed again and dehydrated at increasing ethanol concentrations (1x 50% EtOH, 3x 70% EtOH, 3x 95 % EtOH, 3x 100% EtOH, 3x 100 % dry EtOH; 5 min in each, Sigma-Aldrich). Cells were resuspended in LRW resin (LR White Resin ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were eluted with 2x Laemmli sample buffer and heated at 95°C for 5 min in the presence of β-mercaptoethanol (Sigma). Whole cell lysates or purified protein samples were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2020Quote: ... tissues were dehydrated in increasing concentration of ethanol (70%, 95% and 100% for 2h each) ending in xylene and then incubated overnight in paraffin (Sigma-Aldrich). The following day ...
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bioRxiv - Developmental Biology 2022Quote: ... if fixed in 95% MeOH/5% GAA washes were done with PBDT (1XPBS/1%BSA/2%DMSO [dimethyl sulfoxide, Sigma Aldrich D8418]/0.5%Tween ...
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...
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bioRxiv - Microbiology 2019Quote: ... The samples were then washed three times with distilled water and dehydrated in a series of ethanol (50, 70, 90, 95, 100, 100% for 10 min each) and hexamethyldisilazane (HMDS, Sigma-Aldrich) (50 and 100% for 10 min each) ...
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bioRxiv - Neuroscience 2019Quote: ... Eyes were enucleated from euthanized mice (sodium pentobarbital, 150 mg kg−1) and dissected in carboxygenated (95% O2 and 5% CO2) Ames’ medium (A1420, Sigma-Aldrich). Retinas were mounted ganglion cell down and maintained in contact with the substrate using a 1 mm nylon mesh ...
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bioRxiv - Cell Biology 2020Quote: ... the wafer was baked at 95°C for 2 min and cooled to room temperature and developed in a propylene glycol monomethyl ether acetate (Sigma Aldrich) bath ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Ab3 sensilla were identified by using three standard diagnostic odorants 98 (all odorants were obtained from Sigma-Aldrich, US, purity > 95%): ethyl hexanoate (CAS # 123-66-0) ...
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bioRxiv - Cell Biology 2020Quote: ... multiwalled carbon nanotubes (MWCNTs, Nanocyl, 95% purity) were added in the Polyacrylonitrile solution and stabilized by adding Triton x100 (Sigma Aldrich) at a weight ratio of 1:50 of MWCNT/Triton ...
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bioRxiv - Plant Biology 2022Quote: ... Over the course of 12 hours they were gradually dehydrated to 95% ethanol and then stained overnight in 1% Acid Fuchsin (Sigma-Aldrich) in 95% ethanol ...
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bioRxiv - Pathology 2022Quote: ... Slides were rehydrated using decreasing graded series of ethanol concentrations (100%, 95%, 80%, 70%), then stained with Harris Hematoxylin (Lerner, 1931382) and Eosin Y (Sigma, H911032). Slides were dehydrated with an increasing graded series of ethanol concentrations (50% ...
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bioRxiv - Neuroscience 2023Quote: ... they were rinsed in distilled H2O (for 30 seconds) and in 95% reagent alcohol (for 30 seconds) before being counterstained with eosin Y solution (HT110116, Sigma, Germany) for 1 min ...
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bioRxiv - Developmental Biology 2023Quote: ... and the remaining un-labelled EdU sites were then saturated by click-it chemistry with an excess of non-fluorescent azide (Azido-methyl-phenyl-sulfide 95%, Sigma, #244546): the reaction was prepared and achieved according to the manufacturer’s recommendations except the standard fluorescent azide was replaced by the non-fluorescent one at a final concentration of 2mM ...
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bioRxiv - Neuroscience 2023Quote: ... 3-Hexanol (CAS #623-37-0, purity >97%, Fluka, Buchs, Switzerland) and Heptanal (CAS #111-71-7, purity >95%, SAFC, Sigma Aldrich) (Table 1) ...
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bioRxiv - Biochemistry 2023Quote: ... Enriched proteins were eluted by either heating the beads with 2X Laemmli buffer containing 4% beta-mercaptoethanol (ΩMe) at 95
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bioRxiv - Cancer Biology 2023Quote: ... anti-rabbit secondary antibodies, Ivermectin (I8898, diluted in 95% ethanol), and Leptomycin B (L2913, diluted in 100% ethanol) were purchased from Sigma Aldrich.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured at 37°C in 5% CO2/95% air in Dulbecco’s modified Eagle’s medium (Sigma, St Louis, MO, USA) containing 10% heat-inactivated fetal bovine serum (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... Mitotic cells (>95% purity as assessed by DAPI staining and microscopy) were obtained using nocodazole shake-off16 (5h; 50 ng/ml; Sigma, M1404). For post-mitotic analyses ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant proteins were denatured at 95℃ for 10 min and then alkylated with 40 mM IAA (final concentration, Sigma-Alrich) in dark at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were immediately subjected to heat-mediated antigen retrieval at 95°C for 10 minutes in 1X pH6.0 sodium citrate buffer (Sigma-Aldrich, #21545) using Micromed T/T Mega Multifunctional Microwave Rapid Histoprocessor (Milestone) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was immediately removed and rapidly immersed in oxygenated (95% O2 – 5% CO2) ice-cold “cutting” solution containing (in mmol/L; all from Sigma, US): 87 NaCl (#S9888) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were diluted in 1X Laemmli sample buffer and heated at 95°C for 10 min in the presence of β-mercaptoethanol (Sigma) (except for OXPHOS cocktail antibody according to manufacturer’s instructions) ...
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bioRxiv - Developmental Biology 2023Quote: ... 95°C and run on 10% SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham Protran Western blotting membranes – Sigma Aldrich) to validate protein quality and specificity.
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bioRxiv - Microbiology 2024Quote: ... The wells were subsequently washed by PBS and washed with crystal violet (CV. The wells were washed and solubilized with 95% of ethanol (Sigma Aldrich) prior to the biomass measurement at OD600nm by the Floustar Omega plate reader ...
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bioRxiv - Neuroscience 2024Quote: ... Eyecups were transported to the electrophysiology lab in a light-sealed container filled with freshly oxygenated (95% O2 and 5% CO2) Ames’ medium (Sigma-Aldrich), supplemented with 6 mM D-glucose and buffered with 22 mM NaHCO3 to maintain a pH of 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... The solution was boiled for 5 min at 95 °C before adding 1.2 mL TBS and 20 µL M2-Flag beads (Sigma; Table S4) and incubated at 4°C for 2 h with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Through the recording chamber there was a continuous flow of carbongenated (95% O2/5% CO2) artificial cerebrospinal fluid (aCSF) (124 mM NaCl (Sigma; S5886), 1.25 mM NaH2PO4 (Sigma ...
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bioRxiv - Genomics 2023Quote: ... cells at 80-95% confluency (one 15 cm dish per gradient) were incubated with 100 µg/mL cycloheximide (Sigma Aldrich, C4859) in media for 10 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Thymi from 4-month-old to 12-year-old individuals were cryopreserved in liquid nitrogen in the following solution: 95% (PBS-5% Dextran 40 (Sigma-Aldrich)) – 5% DMSO (Fisher Scientific) ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... UM cells were transduced with virus supplemented with 8 µg/ml polybrene (Sigma Aldrich, Saint Louis, Missouri, USA) and 24 h after transduction ...
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bioRxiv - Cell Biology 2020Quote: ... McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich). After 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNA was reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Sigma) using dNTP (Promega ...
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bioRxiv - Immunology 2021Quote: ... The swabs were placed into 2ml of virus transport medium (VTM) comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... the cell culture supernatants were pooled to concentrate the virus 20 times using 10,000 MWCO spin columns (Millipore), and aliquots were stored at −70°C [14] ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... P2 virus was then harvested by pelleting cells by centrifugation at 4000g and decanting into 5% FBS (Sigma), and then stored in the dark at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
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bioRxiv - Cell Biology 2022Quote: ... Infectious media containing virus was collected 40 hrs later and filtered with a 0.45μm PES membrane filter (Millipore). Live filtered virus was used to transduce cells with polybrene (10μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Cancer Biology 2022Quote: ... the virus-containing supernatant was centrifuged 500 x g for 5min and filtered with 0.45um PES filters (Millipore), followed by ultracentrifugation (25,000rpm for 2h) ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Genetics 2020Quote: ... 10 μL of each virus was diluted in growth media containing polybrene (10 μg/mL; Sigma-Aldrich, USA) then mixed with cells ...
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Genomics 2021Quote: ... We filtered and concentrated the virus in PBS using Amicon Ultra-15 centrifugation filters (Millipore, Burlington, MA; #UFC905024). The viral titer was measured with the AAVpro(R ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... The virus was passaged twice in Vero E6 cell line (ATCC) maintained in DMEM High Glucose (Sigma-Aldrich) supplemented with 2% heat-inactivated Fetal Bovine Serum (Thermo Scientific ...